Job ID = 14522023 SRX = SRX6917496 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8795677 spots for SRR10197354/SRR10197354.sra Written 8795677 spots for SRR10197354/SRR10197354.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:10 8795677 reads; of these: 8795677 (100.00%) were paired; of these: 338068 (3.84%) aligned concordantly 0 times 7554819 (85.89%) aligned concordantly exactly 1 time 902790 (10.26%) aligned concordantly >1 times ---- 338068 pairs aligned concordantly 0 times; of these: 46192 (13.66%) aligned discordantly 1 time ---- 291876 pairs aligned 0 times concordantly or discordantly; of these: 583752 mates make up the pairs; of these: 450227 (77.13%) aligned 0 times 106220 (18.20%) aligned exactly 1 time 27305 (4.68%) aligned >1 times 97.44% overall alignment rate Time searching: 00:12:10 Overall time: 00:12:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1872344 / 8431594 = 0.2221 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:24:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:24:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:24:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:24:30: 1000000 INFO @ Sat, 15 Jan 2022 22:24:38: 2000000 INFO @ Sat, 15 Jan 2022 22:24:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:24:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:24:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:24:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:24:54: 4000000 INFO @ Sat, 15 Jan 2022 22:25:01: 1000000 INFO @ Sat, 15 Jan 2022 22:25:02: 5000000 INFO @ Sat, 15 Jan 2022 22:25:11: 6000000 INFO @ Sat, 15 Jan 2022 22:25:11: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:25:19: 7000000 INFO @ Sat, 15 Jan 2022 22:25:21: 3000000 INFO @ Sat, 15 Jan 2022 22:25:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:25:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:25:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:25:27: 8000000 INFO @ Sat, 15 Jan 2022 22:25:30: 1000000 INFO @ Sat, 15 Jan 2022 22:25:31: 4000000 INFO @ Sat, 15 Jan 2022 22:25:36: 9000000 INFO @ Sat, 15 Jan 2022 22:25:39: 2000000 INFO @ Sat, 15 Jan 2022 22:25:41: 5000000 INFO @ Sat, 15 Jan 2022 22:25:45: 10000000 INFO @ Sat, 15 Jan 2022 22:25:48: 3000000 INFO @ Sat, 15 Jan 2022 22:25:52: 6000000 INFO @ Sat, 15 Jan 2022 22:25:53: 11000000 INFO @ Sat, 15 Jan 2022 22:25:57: 4000000 INFO @ Sat, 15 Jan 2022 22:26:02: 7000000 INFO @ Sat, 15 Jan 2022 22:26:03: 12000000 INFO @ Sat, 15 Jan 2022 22:26:06: 5000000 INFO @ Sat, 15 Jan 2022 22:26:12: 8000000 INFO @ Sat, 15 Jan 2022 22:26:12: 13000000 INFO @ Sat, 15 Jan 2022 22:26:14: 6000000 INFO @ Sat, 15 Jan 2022 22:26:16: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:26:16: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:26:16: #1 total tags in treatment: 6587419 INFO @ Sat, 15 Jan 2022 22:26:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:26:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:26:16: #1 tags after filtering in treatment: 4117126 INFO @ Sat, 15 Jan 2022 22:26:16: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:26:16: #1 finished! INFO @ Sat, 15 Jan 2022 22:26:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:26:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:26:16: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:26:16: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:26:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:26:22: 9000000 INFO @ Sat, 15 Jan 2022 22:26:23: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:26:32: 8000000 INFO @ Sat, 15 Jan 2022 22:26:32: 10000000 INFO @ Sat, 15 Jan 2022 22:26:40: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:26:42: 11000000 INFO @ Sat, 15 Jan 2022 22:26:48: 10000000 INFO @ Sat, 15 Jan 2022 22:26:52: 12000000 INFO @ Sat, 15 Jan 2022 22:26:57: 11000000 INFO @ Sat, 15 Jan 2022 22:27:01: 13000000 INFO @ Sat, 15 Jan 2022 22:27:05: 12000000 INFO @ Sat, 15 Jan 2022 22:27:05: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:27:05: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:27:05: #1 total tags in treatment: 6587419 INFO @ Sat, 15 Jan 2022 22:27:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:27:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:27:05: #1 tags after filtering in treatment: 4117126 INFO @ Sat, 15 Jan 2022 22:27:05: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:27:05: #1 finished! INFO @ Sat, 15 Jan 2022 22:27:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:27:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:27:06: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:27:06: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:27:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:27:13: 13000000 INFO @ Sat, 15 Jan 2022 22:27:16: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:27:16: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:27:16: #1 total tags in treatment: 6587419 INFO @ Sat, 15 Jan 2022 22:27:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:27:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:27:16: #1 tags after filtering in treatment: 4117126 INFO @ Sat, 15 Jan 2022 22:27:16: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 22:27:16: #1 finished! INFO @ Sat, 15 Jan 2022 22:27:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:27:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:27:17: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:27:17: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:27:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917496/SRX6917496.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling