Job ID = 7107552 SRX = SRX6911554 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13470056 spots for SRR10191181/SRR10191181.sra Written 13470056 spots for SRR10191181/SRR10191181.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:07 13470056 reads; of these: 13470056 (100.00%) were paired; of these: 3378490 (25.08%) aligned concordantly 0 times 9023490 (66.99%) aligned concordantly exactly 1 time 1068076 (7.93%) aligned concordantly >1 times ---- 3378490 pairs aligned concordantly 0 times; of these: 1828147 (54.11%) aligned discordantly 1 time ---- 1550343 pairs aligned 0 times concordantly or discordantly; of these: 3100686 mates make up the pairs; of these: 1755626 (56.62%) aligned 0 times 814868 (26.28%) aligned exactly 1 time 530192 (17.10%) aligned >1 times 93.48% overall alignment rate Time searching: 00:14:07 Overall time: 00:14:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 503670 / 11911211 = 0.0423 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:33:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:33:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:33:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:33:59: 1000000 INFO @ Wed, 22 Jul 2020 13:34:08: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:34:18: 3000000 INFO @ Wed, 22 Jul 2020 13:34:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:34:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:34:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:34:28: 4000000 INFO @ Wed, 22 Jul 2020 13:34:29: 1000000 INFO @ Wed, 22 Jul 2020 13:34:37: 5000000 INFO @ Wed, 22 Jul 2020 13:34:38: 2000000 INFO @ Wed, 22 Jul 2020 13:34:46: 6000000 INFO @ Wed, 22 Jul 2020 13:34:47: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:34:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:34:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:34:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:34:55: 7000000 INFO @ Wed, 22 Jul 2020 13:34:57: 4000000 INFO @ Wed, 22 Jul 2020 13:34:59: 1000000 INFO @ Wed, 22 Jul 2020 13:35:06: 8000000 INFO @ Wed, 22 Jul 2020 13:35:08: 5000000 INFO @ Wed, 22 Jul 2020 13:35:08: 2000000 INFO @ Wed, 22 Jul 2020 13:35:17: 9000000 INFO @ Wed, 22 Jul 2020 13:35:17: 3000000 INFO @ Wed, 22 Jul 2020 13:35:19: 6000000 INFO @ Wed, 22 Jul 2020 13:35:27: 4000000 INFO @ Wed, 22 Jul 2020 13:35:27: 10000000 INFO @ Wed, 22 Jul 2020 13:35:29: 7000000 INFO @ Wed, 22 Jul 2020 13:35:36: 5000000 INFO @ Wed, 22 Jul 2020 13:35:38: 11000000 INFO @ Wed, 22 Jul 2020 13:35:40: 8000000 INFO @ Wed, 22 Jul 2020 13:35:45: 6000000 INFO @ Wed, 22 Jul 2020 13:35:49: 12000000 INFO @ Wed, 22 Jul 2020 13:35:51: 9000000 INFO @ Wed, 22 Jul 2020 13:35:54: 7000000 INFO @ Wed, 22 Jul 2020 13:35:59: 13000000 INFO @ Wed, 22 Jul 2020 13:36:02: 10000000 INFO @ Wed, 22 Jul 2020 13:36:03: 8000000 INFO @ Wed, 22 Jul 2020 13:36:10: 14000000 INFO @ Wed, 22 Jul 2020 13:36:12: 9000000 INFO @ Wed, 22 Jul 2020 13:36:12: 11000000 INFO @ Wed, 22 Jul 2020 13:36:20: 15000000 INFO @ Wed, 22 Jul 2020 13:36:21: 10000000 INFO @ Wed, 22 Jul 2020 13:36:23: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:36:31: 11000000 INFO @ Wed, 22 Jul 2020 13:36:31: 16000000 INFO @ Wed, 22 Jul 2020 13:36:33: 13000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:36:40: 12000000 INFO @ Wed, 22 Jul 2020 13:36:41: 17000000 INFO @ Wed, 22 Jul 2020 13:36:44: 14000000 INFO @ Wed, 22 Jul 2020 13:36:49: 13000000 INFO @ Wed, 22 Jul 2020 13:36:51: 18000000 INFO @ Wed, 22 Jul 2020 13:36:54: 15000000 INFO @ Wed, 22 Jul 2020 13:36:58: 14000000 INFO @ Wed, 22 Jul 2020 13:37:02: 19000000 INFO @ Wed, 22 Jul 2020 13:37:05: 16000000 INFO @ Wed, 22 Jul 2020 13:37:08: 15000000 INFO @ Wed, 22 Jul 2020 13:37:12: 20000000 INFO @ Wed, 22 Jul 2020 13:37:15: 17000000 INFO @ Wed, 22 Jul 2020 13:37:17: 16000000 INFO @ Wed, 22 Jul 2020 13:37:23: 21000000 INFO @ Wed, 22 Jul 2020 13:37:26: 18000000 INFO @ Wed, 22 Jul 2020 13:37:26: 17000000 INFO @ Wed, 22 Jul 2020 13:37:33: 22000000 INFO @ Wed, 22 Jul 2020 13:37:35: 18000000 INFO @ Wed, 22 Jul 2020 13:37:36: 19000000 INFO @ Wed, 22 Jul 2020 13:37:43: 23000000 INFO @ Wed, 22 Jul 2020 13:37:45: 19000000 INFO @ Wed, 22 Jul 2020 13:37:47: 20000000 INFO @ Wed, 22 Jul 2020 13:37:53: 24000000 INFO @ Wed, 22 Jul 2020 13:37:54: 20000000 INFO @ Wed, 22 Jul 2020 13:37:55: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:37:55: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:37:55: #1 total tags in treatment: 9623489 INFO @ Wed, 22 Jul 2020 13:37:55: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:37:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:37:55: #1 tags after filtering in treatment: 6408712 INFO @ Wed, 22 Jul 2020 13:37:55: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 13:37:55: #1 finished! INFO @ Wed, 22 Jul 2020 13:37:55: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:37:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:37:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:37:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:37:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:37:57: 21000000 INFO @ Wed, 22 Jul 2020 13:38:03: 21000000 INFO @ Wed, 22 Jul 2020 13:38:07: 22000000 INFO @ Wed, 22 Jul 2020 13:38:12: 22000000 INFO @ Wed, 22 Jul 2020 13:38:16: 23000000 INFO @ Wed, 22 Jul 2020 13:38:22: 23000000 INFO @ Wed, 22 Jul 2020 13:38:26: 24000000 INFO @ Wed, 22 Jul 2020 13:38:28: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:38:28: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:38:28: #1 total tags in treatment: 9623489 INFO @ Wed, 22 Jul 2020 13:38:28: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:38:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:38:28: #1 tags after filtering in treatment: 6408712 INFO @ Wed, 22 Jul 2020 13:38:28: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 13:38:28: #1 finished! INFO @ Wed, 22 Jul 2020 13:38:28: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:38:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:38:28: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:38:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:38:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:38:31: 24000000 INFO @ Wed, 22 Jul 2020 13:38:32: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:38:32: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:38:32: #1 total tags in treatment: 9623489 INFO @ Wed, 22 Jul 2020 13:38:32: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:38:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:38:32: #1 tags after filtering in treatment: 6408712 INFO @ Wed, 22 Jul 2020 13:38:32: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 13:38:32: #1 finished! INFO @ Wed, 22 Jul 2020 13:38:32: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:38:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:38:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:38:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:38:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911554/SRX6911554.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling