Job ID = 7107513 SRX = SRX6911553 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18451748 spots for SRR10191180/SRR10191180.sra Written 18451748 spots for SRR10191180/SRR10191180.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:47 18451748 reads; of these: 18451748 (100.00%) were paired; of these: 5077638 (27.52%) aligned concordantly 0 times 11297719 (61.23%) aligned concordantly exactly 1 time 2076391 (11.25%) aligned concordantly >1 times ---- 5077638 pairs aligned concordantly 0 times; of these: 2074303 (40.85%) aligned discordantly 1 time ---- 3003335 pairs aligned 0 times concordantly or discordantly; of these: 6006670 mates make up the pairs; of these: 4160074 (69.26%) aligned 0 times 936934 (15.60%) aligned exactly 1 time 909662 (15.14%) aligned >1 times 88.73% overall alignment rate Time searching: 00:18:47 Overall time: 00:18:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 851118 / 15441091 = 0.0551 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:40:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:40:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:40:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:40:18: 1000000 INFO @ Wed, 22 Jul 2020 13:40:24: 2000000 INFO @ Wed, 22 Jul 2020 13:40:30: 3000000 INFO @ Wed, 22 Jul 2020 13:40:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:40:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:40:41: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:40:41: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:40:42: 5000000 INFO @ Wed, 22 Jul 2020 13:40:48: 1000000 INFO @ Wed, 22 Jul 2020 13:40:49: 6000000 INFO @ Wed, 22 Jul 2020 13:40:56: 7000000 INFO @ Wed, 22 Jul 2020 13:40:56: 2000000 INFO @ Wed, 22 Jul 2020 13:41:02: 8000000 INFO @ Wed, 22 Jul 2020 13:41:04: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:41:09: 9000000 INFO @ Wed, 22 Jul 2020 13:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:41:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:41:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:41:12: 4000000 INFO @ Wed, 22 Jul 2020 13:41:16: 10000000 INFO @ Wed, 22 Jul 2020 13:41:19: 1000000 INFO @ Wed, 22 Jul 2020 13:41:19: 5000000 INFO @ Wed, 22 Jul 2020 13:41:23: 11000000 INFO @ Wed, 22 Jul 2020 13:41:27: 6000000 INFO @ Wed, 22 Jul 2020 13:41:28: 2000000 INFO @ Wed, 22 Jul 2020 13:41:30: 12000000 INFO @ Wed, 22 Jul 2020 13:41:35: 3000000 INFO @ Wed, 22 Jul 2020 13:41:36: 7000000 INFO @ Wed, 22 Jul 2020 13:41:37: 13000000 INFO @ Wed, 22 Jul 2020 13:41:43: 4000000 INFO @ Wed, 22 Jul 2020 13:41:44: 14000000 INFO @ Wed, 22 Jul 2020 13:41:44: 8000000 INFO @ Wed, 22 Jul 2020 13:41:50: 15000000 INFO @ Wed, 22 Jul 2020 13:41:51: 5000000 INFO @ Wed, 22 Jul 2020 13:41:52: 9000000 INFO @ Wed, 22 Jul 2020 13:41:57: 16000000 INFO @ Wed, 22 Jul 2020 13:41:59: 6000000 INFO @ Wed, 22 Jul 2020 13:42:00: 10000000 INFO @ Wed, 22 Jul 2020 13:42:04: 17000000 INFO @ Wed, 22 Jul 2020 13:42:07: 7000000 INFO @ Wed, 22 Jul 2020 13:42:08: 11000000 INFO @ Wed, 22 Jul 2020 13:42:11: 18000000 INFO @ Wed, 22 Jul 2020 13:42:15: 8000000 INFO @ Wed, 22 Jul 2020 13:42:16: 12000000 INFO @ Wed, 22 Jul 2020 13:42:18: 19000000 INFO @ Wed, 22 Jul 2020 13:42:22: 9000000 INFO @ Wed, 22 Jul 2020 13:42:24: 13000000 INFO @ Wed, 22 Jul 2020 13:42:25: 20000000 INFO @ Wed, 22 Jul 2020 13:42:30: 10000000 INFO @ Wed, 22 Jul 2020 13:42:32: 21000000 INFO @ Wed, 22 Jul 2020 13:42:32: 14000000 INFO @ Wed, 22 Jul 2020 13:42:38: 11000000 INFO @ Wed, 22 Jul 2020 13:42:38: 22000000 INFO @ Wed, 22 Jul 2020 13:42:40: 15000000 INFO @ Wed, 22 Jul 2020 13:42:45: 23000000 INFO @ Wed, 22 Jul 2020 13:42:46: 12000000 INFO @ Wed, 22 Jul 2020 13:42:48: 16000000 INFO @ Wed, 22 Jul 2020 13:42:52: 24000000 INFO @ Wed, 22 Jul 2020 13:42:54: 13000000 INFO @ Wed, 22 Jul 2020 13:42:55: 17000000 INFO @ Wed, 22 Jul 2020 13:42:59: 25000000 INFO @ Wed, 22 Jul 2020 13:43:02: 14000000 INFO @ Wed, 22 Jul 2020 13:43:03: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:43:06: 26000000 INFO @ Wed, 22 Jul 2020 13:43:10: 15000000 INFO @ Wed, 22 Jul 2020 13:43:10: 19000000 INFO @ Wed, 22 Jul 2020 13:43:13: 27000000 INFO @ Wed, 22 Jul 2020 13:43:18: 16000000 INFO @ Wed, 22 Jul 2020 13:43:19: 20000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:43:19: 28000000 INFO @ Wed, 22 Jul 2020 13:43:26: 29000000 INFO @ Wed, 22 Jul 2020 13:43:26: 21000000 INFO @ Wed, 22 Jul 2020 13:43:26: 17000000 INFO @ Wed, 22 Jul 2020 13:43:33: 30000000 INFO @ Wed, 22 Jul 2020 13:43:34: 22000000 INFO @ Wed, 22 Jul 2020 13:43:35: 18000000 INFO @ Wed, 22 Jul 2020 13:43:40: 31000000 INFO @ Wed, 22 Jul 2020 13:43:41: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:43:41: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:43:41: #1 total tags in treatment: 12562050 INFO @ Wed, 22 Jul 2020 13:43:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:43:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:43:41: #1 tags after filtering in treatment: 8027549 INFO @ Wed, 22 Jul 2020 13:43:41: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:43:41: #1 finished! INFO @ Wed, 22 Jul 2020 13:43:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:43:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:43:41: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:43:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:43:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:43:42: 23000000 INFO @ Wed, 22 Jul 2020 13:43:43: 19000000 INFO @ Wed, 22 Jul 2020 13:43:50: 24000000 INFO @ Wed, 22 Jul 2020 13:43:51: 20000000 INFO @ Wed, 22 Jul 2020 13:43:58: 25000000 INFO @ Wed, 22 Jul 2020 13:43:59: 21000000 INFO @ Wed, 22 Jul 2020 13:44:06: 26000000 INFO @ Wed, 22 Jul 2020 13:44:07: 22000000 INFO @ Wed, 22 Jul 2020 13:44:14: 27000000 INFO @ Wed, 22 Jul 2020 13:44:14: 23000000 INFO @ Wed, 22 Jul 2020 13:44:22: 28000000 INFO @ Wed, 22 Jul 2020 13:44:22: 24000000 INFO @ Wed, 22 Jul 2020 13:44:30: 29000000 INFO @ Wed, 22 Jul 2020 13:44:30: 25000000 INFO @ Wed, 22 Jul 2020 13:44:38: 30000000 INFO @ Wed, 22 Jul 2020 13:44:38: 26000000 INFO @ Wed, 22 Jul 2020 13:44:46: 31000000 INFO @ Wed, 22 Jul 2020 13:44:46: 27000000 INFO @ Wed, 22 Jul 2020 13:44:46: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:44:46: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:44:46: #1 total tags in treatment: 12562050 INFO @ Wed, 22 Jul 2020 13:44:46: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:44:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:44:46: #1 tags after filtering in treatment: 8027549 INFO @ Wed, 22 Jul 2020 13:44:46: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:44:46: #1 finished! INFO @ Wed, 22 Jul 2020 13:44:46: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:44:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:44:47: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:44:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:44:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:44:54: 28000000 INFO @ Wed, 22 Jul 2020 13:45:01: 29000000 INFO @ Wed, 22 Jul 2020 13:45:09: 30000000 INFO @ Wed, 22 Jul 2020 13:45:16: 31000000 INFO @ Wed, 22 Jul 2020 13:45:17: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:45:17: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:45:17: #1 total tags in treatment: 12562050 INFO @ Wed, 22 Jul 2020 13:45:17: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:45:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:45:17: #1 tags after filtering in treatment: 8027549 INFO @ Wed, 22 Jul 2020 13:45:17: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:45:17: #1 finished! INFO @ Wed, 22 Jul 2020 13:45:17: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:45:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:45:17: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:45:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:45:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911553/SRX6911553.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling