Job ID = 7107509 SRX = SRX6911552 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19071489 spots for SRR10191179/SRR10191179.sra Written 19071489 spots for SRR10191179/SRR10191179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:16 19071489 reads; of these: 19071489 (100.00%) were paired; of these: 4359925 (22.86%) aligned concordantly 0 times 12646367 (66.31%) aligned concordantly exactly 1 time 2065197 (10.83%) aligned concordantly >1 times ---- 4359925 pairs aligned concordantly 0 times; of these: 2193165 (50.30%) aligned discordantly 1 time ---- 2166760 pairs aligned 0 times concordantly or discordantly; of these: 4333520 mates make up the pairs; of these: 2677365 (61.78%) aligned 0 times 854533 (19.72%) aligned exactly 1 time 801622 (18.50%) aligned >1 times 92.98% overall alignment rate Time searching: 00:24:16 Overall time: 00:24:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 457780 / 16898179 = 0.0271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:45:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:45:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:45:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:45:29: 1000000 INFO @ Wed, 22 Jul 2020 13:45:36: 2000000 INFO @ Wed, 22 Jul 2020 13:45:43: 3000000 INFO @ Wed, 22 Jul 2020 13:45:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:45:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:45:53: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:45:53: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:45:58: 5000000 INFO @ Wed, 22 Jul 2020 13:46:01: 1000000 INFO @ Wed, 22 Jul 2020 13:46:06: 6000000 INFO @ Wed, 22 Jul 2020 13:46:09: 2000000 INFO @ Wed, 22 Jul 2020 13:46:14: 7000000 INFO @ Wed, 22 Jul 2020 13:46:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:46:22: 8000000 INFO @ Wed, 22 Jul 2020 13:46:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:46:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:46:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:46:25: 4000000 INFO @ Wed, 22 Jul 2020 13:46:30: 9000000 INFO @ Wed, 22 Jul 2020 13:46:31: 1000000 INFO @ Wed, 22 Jul 2020 13:46:33: 5000000 INFO @ Wed, 22 Jul 2020 13:46:38: 10000000 INFO @ Wed, 22 Jul 2020 13:46:39: 2000000 INFO @ Wed, 22 Jul 2020 13:46:41: 6000000 INFO @ Wed, 22 Jul 2020 13:46:46: 11000000 INFO @ Wed, 22 Jul 2020 13:46:47: 3000000 INFO @ Wed, 22 Jul 2020 13:46:49: 7000000 INFO @ Wed, 22 Jul 2020 13:46:54: 12000000 INFO @ Wed, 22 Jul 2020 13:46:55: 4000000 INFO @ Wed, 22 Jul 2020 13:46:57: 8000000 INFO @ Wed, 22 Jul 2020 13:47:02: 13000000 INFO @ Wed, 22 Jul 2020 13:47:03: 5000000 INFO @ Wed, 22 Jul 2020 13:47:04: 9000000 INFO @ Wed, 22 Jul 2020 13:47:10: 14000000 INFO @ Wed, 22 Jul 2020 13:47:11: 6000000 INFO @ Wed, 22 Jul 2020 13:47:12: 10000000 INFO @ Wed, 22 Jul 2020 13:47:18: 15000000 INFO @ Wed, 22 Jul 2020 13:47:18: 7000000 INFO @ Wed, 22 Jul 2020 13:47:20: 11000000 INFO @ Wed, 22 Jul 2020 13:47:26: 16000000 INFO @ Wed, 22 Jul 2020 13:47:26: 8000000 INFO @ Wed, 22 Jul 2020 13:47:28: 12000000 INFO @ Wed, 22 Jul 2020 13:47:34: 17000000 INFO @ Wed, 22 Jul 2020 13:47:34: 9000000 INFO @ Wed, 22 Jul 2020 13:47:36: 13000000 INFO @ Wed, 22 Jul 2020 13:47:41: 18000000 INFO @ Wed, 22 Jul 2020 13:47:42: 10000000 INFO @ Wed, 22 Jul 2020 13:47:43: 14000000 INFO @ Wed, 22 Jul 2020 13:47:49: 19000000 INFO @ Wed, 22 Jul 2020 13:47:50: 11000000 INFO @ Wed, 22 Jul 2020 13:47:51: 15000000 INFO @ Wed, 22 Jul 2020 13:47:57: 20000000 INFO @ Wed, 22 Jul 2020 13:47:58: 12000000 INFO @ Wed, 22 Jul 2020 13:47:59: 16000000 INFO @ Wed, 22 Jul 2020 13:48:05: 21000000 INFO @ Wed, 22 Jul 2020 13:48:06: 13000000 INFO @ Wed, 22 Jul 2020 13:48:07: 17000000 INFO @ Wed, 22 Jul 2020 13:48:13: 22000000 INFO @ Wed, 22 Jul 2020 13:48:13: 14000000 INFO @ Wed, 22 Jul 2020 13:48:15: 18000000 INFO @ Wed, 22 Jul 2020 13:48:21: 23000000 INFO @ Wed, 22 Jul 2020 13:48:21: 15000000 INFO @ Wed, 22 Jul 2020 13:48:23: 19000000 INFO @ Wed, 22 Jul 2020 13:48:29: 24000000 INFO @ Wed, 22 Jul 2020 13:48:29: 16000000 INFO @ Wed, 22 Jul 2020 13:48:31: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:48:37: 25000000 INFO @ Wed, 22 Jul 2020 13:48:37: 17000000 INFO @ Wed, 22 Jul 2020 13:48:39: 21000000 INFO @ Wed, 22 Jul 2020 13:48:45: 26000000 INFO @ Wed, 22 Jul 2020 13:48:45: 18000000 INFO @ Wed, 22 Jul 2020 13:48:46: 22000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:48:53: 27000000 INFO @ Wed, 22 Jul 2020 13:48:53: 19000000 INFO @ Wed, 22 Jul 2020 13:48:54: 23000000 INFO @ Wed, 22 Jul 2020 13:49:01: 28000000 INFO @ Wed, 22 Jul 2020 13:49:01: 20000000 INFO @ Wed, 22 Jul 2020 13:49:02: 24000000 INFO @ Wed, 22 Jul 2020 13:49:09: 29000000 INFO @ Wed, 22 Jul 2020 13:49:09: 21000000 INFO @ Wed, 22 Jul 2020 13:49:10: 25000000 INFO @ Wed, 22 Jul 2020 13:49:16: 30000000 INFO @ Wed, 22 Jul 2020 13:49:17: 22000000 INFO @ Wed, 22 Jul 2020 13:49:17: 26000000 INFO @ Wed, 22 Jul 2020 13:49:24: 31000000 INFO @ Wed, 22 Jul 2020 13:49:24: 23000000 INFO @ Wed, 22 Jul 2020 13:49:25: 27000000 INFO @ Wed, 22 Jul 2020 13:49:32: 32000000 INFO @ Wed, 22 Jul 2020 13:49:33: 24000000 INFO @ Wed, 22 Jul 2020 13:49:33: 28000000 INFO @ Wed, 22 Jul 2020 13:49:40: 33000000 INFO @ Wed, 22 Jul 2020 13:49:41: 25000000 INFO @ Wed, 22 Jul 2020 13:49:41: 29000000 INFO @ Wed, 22 Jul 2020 13:49:48: 34000000 INFO @ Wed, 22 Jul 2020 13:49:49: 26000000 INFO @ Wed, 22 Jul 2020 13:49:49: 30000000 INFO @ Wed, 22 Jul 2020 13:49:53: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:49:53: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:49:53: #1 total tags in treatment: 14264156 INFO @ Wed, 22 Jul 2020 13:49:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:49:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:49:53: #1 tags after filtering in treatment: 8868172 INFO @ Wed, 22 Jul 2020 13:49:53: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Jul 2020 13:49:53: #1 finished! INFO @ Wed, 22 Jul 2020 13:49:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:49:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:49:54: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:49:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:49:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:49:57: 27000000 INFO @ Wed, 22 Jul 2020 13:49:57: 31000000 INFO @ Wed, 22 Jul 2020 13:50:05: 28000000 INFO @ Wed, 22 Jul 2020 13:50:05: 32000000 INFO @ Wed, 22 Jul 2020 13:50:12: 29000000 INFO @ Wed, 22 Jul 2020 13:50:13: 33000000 INFO @ Wed, 22 Jul 2020 13:50:20: 30000000 INFO @ Wed, 22 Jul 2020 13:50:22: 34000000 INFO @ Wed, 22 Jul 2020 13:50:26: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:50:26: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:50:26: #1 total tags in treatment: 14264156 INFO @ Wed, 22 Jul 2020 13:50:26: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:50:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:50:27: #1 tags after filtering in treatment: 8868172 INFO @ Wed, 22 Jul 2020 13:50:27: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Jul 2020 13:50:27: #1 finished! INFO @ Wed, 22 Jul 2020 13:50:27: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:50:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:50:27: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:50:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:50:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:50:28: 31000000 INFO @ Wed, 22 Jul 2020 13:50:36: 32000000 INFO @ Wed, 22 Jul 2020 13:50:43: 33000000 INFO @ Wed, 22 Jul 2020 13:50:51: 34000000 INFO @ Wed, 22 Jul 2020 13:50:55: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:50:55: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:50:55: #1 total tags in treatment: 14264156 INFO @ Wed, 22 Jul 2020 13:50:55: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:50:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:50:55: #1 tags after filtering in treatment: 8868172 INFO @ Wed, 22 Jul 2020 13:50:55: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Jul 2020 13:50:55: #1 finished! INFO @ Wed, 22 Jul 2020 13:50:55: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:50:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:50:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:50:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:50:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911552/SRX6911552.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling