Job ID = 7106999 SRX = SRX6911542 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29732895 spots for SRR10191153/SRR10191153.sra Written 29732895 spots for SRR10191153/SRR10191153.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:45 29732895 reads; of these: 29732895 (100.00%) were paired; of these: 6125195 (20.60%) aligned concordantly 0 times 17720049 (59.60%) aligned concordantly exactly 1 time 5887651 (19.80%) aligned concordantly >1 times ---- 6125195 pairs aligned concordantly 0 times; of these: 2069807 (33.79%) aligned discordantly 1 time ---- 4055388 pairs aligned 0 times concordantly or discordantly; of these: 8110776 mates make up the pairs; of these: 6060072 (74.72%) aligned 0 times 788403 (9.72%) aligned exactly 1 time 1262301 (15.56%) aligned >1 times 89.81% overall alignment rate Time searching: 00:35:45 Overall time: 00:35:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3391426 / 25672035 = 0.1321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:50:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:50:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:51:01: 1000000 INFO @ Wed, 22 Jul 2020 13:51:08: 2000000 INFO @ Wed, 22 Jul 2020 13:51:15: 3000000 INFO @ Wed, 22 Jul 2020 13:51:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:51:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:51:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:51:29: 5000000 INFO @ Wed, 22 Jul 2020 13:51:32: 1000000 INFO @ Wed, 22 Jul 2020 13:51:37: 6000000 INFO @ Wed, 22 Jul 2020 13:51:40: 2000000 INFO @ Wed, 22 Jul 2020 13:51:45: 7000000 INFO @ Wed, 22 Jul 2020 13:51:48: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:51:53: 8000000 INFO @ Wed, 22 Jul 2020 13:51:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:51:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:51:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:51:56: 4000000 INFO @ Wed, 22 Jul 2020 13:52:01: 9000000 INFO @ Wed, 22 Jul 2020 13:52:02: 1000000 INFO @ Wed, 22 Jul 2020 13:52:04: 5000000 INFO @ Wed, 22 Jul 2020 13:52:09: 10000000 INFO @ Wed, 22 Jul 2020 13:52:10: 2000000 INFO @ Wed, 22 Jul 2020 13:52:12: 6000000 INFO @ Wed, 22 Jul 2020 13:52:17: 11000000 INFO @ Wed, 22 Jul 2020 13:52:18: 3000000 INFO @ Wed, 22 Jul 2020 13:52:20: 7000000 INFO @ Wed, 22 Jul 2020 13:52:24: 12000000 INFO @ Wed, 22 Jul 2020 13:52:26: 4000000 INFO @ Wed, 22 Jul 2020 13:52:27: 8000000 INFO @ Wed, 22 Jul 2020 13:52:32: 13000000 INFO @ Wed, 22 Jul 2020 13:52:34: 5000000 INFO @ Wed, 22 Jul 2020 13:52:35: 9000000 INFO @ Wed, 22 Jul 2020 13:52:40: 14000000 INFO @ Wed, 22 Jul 2020 13:52:42: 6000000 INFO @ Wed, 22 Jul 2020 13:52:43: 10000000 INFO @ Wed, 22 Jul 2020 13:52:48: 15000000 INFO @ Wed, 22 Jul 2020 13:52:50: 7000000 INFO @ Wed, 22 Jul 2020 13:52:51: 11000000 INFO @ Wed, 22 Jul 2020 13:52:56: 16000000 INFO @ Wed, 22 Jul 2020 13:52:58: 8000000 INFO @ Wed, 22 Jul 2020 13:52:59: 12000000 INFO @ Wed, 22 Jul 2020 13:53:04: 17000000 INFO @ Wed, 22 Jul 2020 13:53:06: 9000000 INFO @ Wed, 22 Jul 2020 13:53:07: 13000000 INFO @ Wed, 22 Jul 2020 13:53:11: 18000000 INFO @ Wed, 22 Jul 2020 13:53:13: 10000000 INFO @ Wed, 22 Jul 2020 13:53:14: 14000000 INFO @ Wed, 22 Jul 2020 13:53:19: 19000000 INFO @ Wed, 22 Jul 2020 13:53:21: 11000000 INFO @ Wed, 22 Jul 2020 13:53:22: 15000000 INFO @ Wed, 22 Jul 2020 13:53:27: 20000000 INFO @ Wed, 22 Jul 2020 13:53:29: 12000000 INFO @ Wed, 22 Jul 2020 13:53:30: 16000000 INFO @ Wed, 22 Jul 2020 13:53:35: 21000000 INFO @ Wed, 22 Jul 2020 13:53:37: 13000000 INFO @ Wed, 22 Jul 2020 13:53:38: 17000000 INFO @ Wed, 22 Jul 2020 13:53:43: 22000000 INFO @ Wed, 22 Jul 2020 13:53:44: 14000000 INFO @ Wed, 22 Jul 2020 13:53:45: 18000000 INFO @ Wed, 22 Jul 2020 13:53:51: 23000000 INFO @ Wed, 22 Jul 2020 13:53:52: 15000000 INFO @ Wed, 22 Jul 2020 13:53:53: 19000000 INFO @ Wed, 22 Jul 2020 13:53:59: 24000000 INFO @ Wed, 22 Jul 2020 13:54:00: 16000000 INFO @ Wed, 22 Jul 2020 13:54:01: 20000000 INFO @ Wed, 22 Jul 2020 13:54:07: 25000000 INFO @ Wed, 22 Jul 2020 13:54:08: 17000000 INFO @ Wed, 22 Jul 2020 13:54:09: 21000000 INFO @ Wed, 22 Jul 2020 13:54:15: 26000000 INFO @ Wed, 22 Jul 2020 13:54:15: 18000000 INFO @ Wed, 22 Jul 2020 13:54:17: 22000000 INFO @ Wed, 22 Jul 2020 13:54:23: 27000000 INFO @ Wed, 22 Jul 2020 13:54:23: 19000000 INFO @ Wed, 22 Jul 2020 13:54:25: 23000000 INFO @ Wed, 22 Jul 2020 13:54:31: 28000000 INFO @ Wed, 22 Jul 2020 13:54:31: 20000000 INFO @ Wed, 22 Jul 2020 13:54:33: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:54:39: 29000000 INFO @ Wed, 22 Jul 2020 13:54:39: 21000000 INFO @ Wed, 22 Jul 2020 13:54:41: 25000000 INFO @ Wed, 22 Jul 2020 13:54:46: 30000000 INFO @ Wed, 22 Jul 2020 13:54:47: 22000000 INFO @ Wed, 22 Jul 2020 13:54:49: 26000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:54:54: 31000000 INFO @ Wed, 22 Jul 2020 13:54:55: 23000000 INFO @ Wed, 22 Jul 2020 13:54:57: 27000000 INFO @ Wed, 22 Jul 2020 13:55:02: 32000000 INFO @ Wed, 22 Jul 2020 13:55:03: 24000000 INFO @ Wed, 22 Jul 2020 13:55:04: 28000000 INFO @ Wed, 22 Jul 2020 13:55:09: 33000000 INFO @ Wed, 22 Jul 2020 13:55:10: 25000000 INFO @ Wed, 22 Jul 2020 13:55:12: 29000000 INFO @ Wed, 22 Jul 2020 13:55:17: 34000000 INFO @ Wed, 22 Jul 2020 13:55:18: 26000000 INFO @ Wed, 22 Jul 2020 13:55:20: 30000000 INFO @ Wed, 22 Jul 2020 13:55:25: 35000000 INFO @ Wed, 22 Jul 2020 13:55:26: 27000000 INFO @ Wed, 22 Jul 2020 13:55:28: 31000000 INFO @ Wed, 22 Jul 2020 13:55:33: 36000000 INFO @ Wed, 22 Jul 2020 13:55:34: 28000000 INFO @ Wed, 22 Jul 2020 13:55:35: 32000000 INFO @ Wed, 22 Jul 2020 13:55:40: 37000000 INFO @ Wed, 22 Jul 2020 13:55:42: 29000000 INFO @ Wed, 22 Jul 2020 13:55:43: 33000000 INFO @ Wed, 22 Jul 2020 13:55:48: 38000000 INFO @ Wed, 22 Jul 2020 13:55:49: 30000000 INFO @ Wed, 22 Jul 2020 13:55:51: 34000000 INFO @ Wed, 22 Jul 2020 13:55:56: 39000000 INFO @ Wed, 22 Jul 2020 13:55:57: 31000000 INFO @ Wed, 22 Jul 2020 13:55:58: 35000000 INFO @ Wed, 22 Jul 2020 13:56:04: 40000000 INFO @ Wed, 22 Jul 2020 13:56:05: 32000000 INFO @ Wed, 22 Jul 2020 13:56:06: 36000000 INFO @ Wed, 22 Jul 2020 13:56:12: 41000000 INFO @ Wed, 22 Jul 2020 13:56:12: 33000000 INFO @ Wed, 22 Jul 2020 13:56:14: 37000000 INFO @ Wed, 22 Jul 2020 13:56:20: 42000000 INFO @ Wed, 22 Jul 2020 13:56:20: 34000000 INFO @ Wed, 22 Jul 2020 13:56:22: 38000000 INFO @ Wed, 22 Jul 2020 13:56:27: 43000000 INFO @ Wed, 22 Jul 2020 13:56:28: 35000000 INFO @ Wed, 22 Jul 2020 13:56:30: 39000000 INFO @ Wed, 22 Jul 2020 13:56:35: 44000000 INFO @ Wed, 22 Jul 2020 13:56:35: 36000000 INFO @ Wed, 22 Jul 2020 13:56:38: 40000000 INFO @ Wed, 22 Jul 2020 13:56:43: 37000000 INFO @ Wed, 22 Jul 2020 13:56:43: 45000000 INFO @ Wed, 22 Jul 2020 13:56:45: 41000000 INFO @ Wed, 22 Jul 2020 13:56:51: 38000000 INFO @ Wed, 22 Jul 2020 13:56:51: 46000000 INFO @ Wed, 22 Jul 2020 13:56:53: 42000000 INFO @ Wed, 22 Jul 2020 13:56:56: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:56:56: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:56:56: #1 total tags in treatment: 20249855 INFO @ Wed, 22 Jul 2020 13:56:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:56:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:56:56: #1 tags after filtering in treatment: 10027384 INFO @ Wed, 22 Jul 2020 13:56:56: #1 Redundant rate of treatment: 0.50 INFO @ Wed, 22 Jul 2020 13:56:56: #1 finished! INFO @ Wed, 22 Jul 2020 13:56:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:56:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:56:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:56:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:56:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:56:59: 39000000 INFO @ Wed, 22 Jul 2020 13:57:01: 43000000 INFO @ Wed, 22 Jul 2020 13:57:06: 40000000 INFO @ Wed, 22 Jul 2020 13:57:09: 44000000 INFO @ Wed, 22 Jul 2020 13:57:14: 41000000 INFO @ Wed, 22 Jul 2020 13:57:17: 45000000 INFO @ Wed, 22 Jul 2020 13:57:22: 42000000 INFO @ Wed, 22 Jul 2020 13:57:24: 46000000 INFO @ Wed, 22 Jul 2020 13:57:29: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:57:29: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:57:29: #1 total tags in treatment: 20249855 INFO @ Wed, 22 Jul 2020 13:57:29: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:57:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:57:30: #1 tags after filtering in treatment: 10027384 INFO @ Wed, 22 Jul 2020 13:57:30: #1 Redundant rate of treatment: 0.50 INFO @ Wed, 22 Jul 2020 13:57:30: #1 finished! INFO @ Wed, 22 Jul 2020 13:57:30: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:57:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:57:30: 43000000 INFO @ Wed, 22 Jul 2020 13:57:30: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:57:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:57:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:57:36: 44000000 INFO @ Wed, 22 Jul 2020 13:57:43: 45000000 INFO @ Wed, 22 Jul 2020 13:57:50: 46000000 INFO @ Wed, 22 Jul 2020 13:57:54: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:57:54: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:57:54: #1 total tags in treatment: 20249855 INFO @ Wed, 22 Jul 2020 13:57:54: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:57:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:57:54: #1 tags after filtering in treatment: 10027384 INFO @ Wed, 22 Jul 2020 13:57:54: #1 Redundant rate of treatment: 0.50 INFO @ Wed, 22 Jul 2020 13:57:54: #1 finished! INFO @ Wed, 22 Jul 2020 13:57:54: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:57:55: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:57:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:57:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911542/SRX6911542.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling