Job ID = 7106899 SRX = SRX6911540 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14943891 spots for SRR10191151/SRR10191151.sra Written 14943891 spots for SRR10191151/SRR10191151.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:30 14943891 reads; of these: 14943891 (100.00%) were paired; of these: 7423326 (49.67%) aligned concordantly 0 times 4366215 (29.22%) aligned concordantly exactly 1 time 3154350 (21.11%) aligned concordantly >1 times ---- 7423326 pairs aligned concordantly 0 times; of these: 602641 (8.12%) aligned discordantly 1 time ---- 6820685 pairs aligned 0 times concordantly or discordantly; of these: 13641370 mates make up the pairs; of these: 12779952 (93.69%) aligned 0 times 214221 (1.57%) aligned exactly 1 time 647197 (4.74%) aligned >1 times 57.24% overall alignment rate Time searching: 00:12:30 Overall time: 00:12:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1292851 / 8120898 = 0.1592 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:11:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:11:04: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:11:04: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:11:10: 1000000 INFO @ Wed, 22 Jul 2020 13:11:15: 2000000 INFO @ Wed, 22 Jul 2020 13:11:20: 3000000 INFO @ Wed, 22 Jul 2020 13:11:26: 4000000 INFO @ Wed, 22 Jul 2020 13:11:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:11:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:11:34: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:11:34: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:11:37: 6000000 INFO @ Wed, 22 Jul 2020 13:11:39: 1000000 INFO @ Wed, 22 Jul 2020 13:11:42: 7000000 INFO @ Wed, 22 Jul 2020 13:11:45: 2000000 INFO @ Wed, 22 Jul 2020 13:11:47: 8000000 INFO @ Wed, 22 Jul 2020 13:11:50: 3000000 INFO @ Wed, 22 Jul 2020 13:11:52: 9000000 INFO @ Wed, 22 Jul 2020 13:11:56: 4000000 INFO @ Wed, 22 Jul 2020 13:11:58: 10000000 INFO @ Wed, 22 Jul 2020 13:12:02: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:12:03: 11000000 INFO @ Wed, 22 Jul 2020 13:12:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:12:04: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:12:04: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:12:07: 6000000 INFO @ Wed, 22 Jul 2020 13:12:08: 12000000 INFO @ Wed, 22 Jul 2020 13:12:09: 1000000 INFO @ Wed, 22 Jul 2020 13:12:13: 7000000 INFO @ Wed, 22 Jul 2020 13:12:14: 13000000 INFO @ Wed, 22 Jul 2020 13:12:15: 2000000 INFO @ Wed, 22 Jul 2020 13:12:18: 8000000 INFO @ Wed, 22 Jul 2020 13:12:19: 14000000 INFO @ Wed, 22 Jul 2020 13:12:21: 3000000 INFO @ Wed, 22 Jul 2020 13:12:22: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:12:22: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:12:22: #1 total tags in treatment: 6252029 INFO @ Wed, 22 Jul 2020 13:12:22: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:12:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:12:22: #1 tags after filtering in treatment: 3737477 INFO @ Wed, 22 Jul 2020 13:12:22: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Jul 2020 13:12:22: #1 finished! INFO @ Wed, 22 Jul 2020 13:12:22: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:12:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:12:23: #2 number of paired peaks: 29 WARNING @ Wed, 22 Jul 2020 13:12:23: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:12:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:12:24: 9000000 INFO @ Wed, 22 Jul 2020 13:12:26: 4000000 INFO @ Wed, 22 Jul 2020 13:12:29: 10000000 INFO @ Wed, 22 Jul 2020 13:12:32: 5000000 INFO @ Wed, 22 Jul 2020 13:12:34: 11000000 INFO @ Wed, 22 Jul 2020 13:12:37: 6000000 INFO @ Wed, 22 Jul 2020 13:12:40: 12000000 INFO @ Wed, 22 Jul 2020 13:12:42: 7000000 INFO @ Wed, 22 Jul 2020 13:12:45: 13000000 INFO @ Wed, 22 Jul 2020 13:12:48: 8000000 INFO @ Wed, 22 Jul 2020 13:12:51: 14000000 INFO @ Wed, 22 Jul 2020 13:12:53: 9000000 INFO @ Wed, 22 Jul 2020 13:12:53: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:12:53: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:12:53: #1 total tags in treatment: 6252029 INFO @ Wed, 22 Jul 2020 13:12:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:12:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:12:54: #1 tags after filtering in treatment: 3737477 INFO @ Wed, 22 Jul 2020 13:12:54: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Jul 2020 13:12:54: #1 finished! INFO @ Wed, 22 Jul 2020 13:12:54: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:12:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:12:54: #2 number of paired peaks: 29 WARNING @ Wed, 22 Jul 2020 13:12:54: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:12:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:12:59: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:13:04: 11000000 INFO @ Wed, 22 Jul 2020 13:13:09: 12000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:13:14: 13000000 INFO @ Wed, 22 Jul 2020 13:13:20: 14000000 INFO @ Wed, 22 Jul 2020 13:13:22: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:13:22: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:13:22: #1 total tags in treatment: 6252029 INFO @ Wed, 22 Jul 2020 13:13:22: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:13:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:13:23: #1 tags after filtering in treatment: 3737477 INFO @ Wed, 22 Jul 2020 13:13:23: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Jul 2020 13:13:23: #1 finished! INFO @ Wed, 22 Jul 2020 13:13:23: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:13:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:13:23: #2 number of paired peaks: 29 WARNING @ Wed, 22 Jul 2020 13:13:23: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:13:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911540/SRX6911540.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling