Job ID = 7106217 SRX = SRX6911535 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29949320 spots for SRR10191146/SRR10191146.sra Written 29949320 spots for SRR10191146/SRR10191146.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:48 29949320 reads; of these: 29949320 (100.00%) were paired; of these: 5978961 (19.96%) aligned concordantly 0 times 19462683 (64.99%) aligned concordantly exactly 1 time 4507676 (15.05%) aligned concordantly >1 times ---- 5978961 pairs aligned concordantly 0 times; of these: 1037032 (17.34%) aligned discordantly 1 time ---- 4941929 pairs aligned 0 times concordantly or discordantly; of these: 9883858 mates make up the pairs; of these: 8158867 (82.55%) aligned 0 times 993093 (10.05%) aligned exactly 1 time 731898 (7.40%) aligned >1 times 86.38% overall alignment rate Time searching: 00:33:48 Overall time: 00:33:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4951850 / 24999227 = 0.1981 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:38:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:38:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:38:53: 1000000 INFO @ Wed, 22 Jul 2020 13:39:00: 2000000 INFO @ Wed, 22 Jul 2020 13:39:07: 3000000 INFO @ Wed, 22 Jul 2020 13:39:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:39:16: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:39:16: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:39:21: 5000000 INFO @ Wed, 22 Jul 2020 13:39:23: 1000000 INFO @ Wed, 22 Jul 2020 13:39:29: 6000000 INFO @ Wed, 22 Jul 2020 13:39:31: 2000000 INFO @ Wed, 22 Jul 2020 13:39:36: 7000000 INFO @ Wed, 22 Jul 2020 13:39:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:39:44: 8000000 INFO @ Wed, 22 Jul 2020 13:39:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:39:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:39:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:39:46: 4000000 INFO @ Wed, 22 Jul 2020 13:39:52: 9000000 INFO @ Wed, 22 Jul 2020 13:39:54: 1000000 INFO @ Wed, 22 Jul 2020 13:39:54: 5000000 INFO @ Wed, 22 Jul 2020 13:40:00: 10000000 INFO @ Wed, 22 Jul 2020 13:40:01: 2000000 INFO @ Wed, 22 Jul 2020 13:40:01: 6000000 INFO @ Wed, 22 Jul 2020 13:40:08: 11000000 INFO @ Wed, 22 Jul 2020 13:40:09: 3000000 INFO @ Wed, 22 Jul 2020 13:40:09: 7000000 INFO @ Wed, 22 Jul 2020 13:40:15: 12000000 INFO @ Wed, 22 Jul 2020 13:40:17: 4000000 INFO @ Wed, 22 Jul 2020 13:40:17: 8000000 INFO @ Wed, 22 Jul 2020 13:40:23: 13000000 INFO @ Wed, 22 Jul 2020 13:40:24: 9000000 INFO @ Wed, 22 Jul 2020 13:40:25: 5000000 INFO @ Wed, 22 Jul 2020 13:40:31: 14000000 INFO @ Wed, 22 Jul 2020 13:40:32: 10000000 INFO @ Wed, 22 Jul 2020 13:40:32: 6000000 INFO @ Wed, 22 Jul 2020 13:40:39: 15000000 INFO @ Wed, 22 Jul 2020 13:40:40: 11000000 INFO @ Wed, 22 Jul 2020 13:40:40: 7000000 INFO @ Wed, 22 Jul 2020 13:40:46: 16000000 INFO @ Wed, 22 Jul 2020 13:40:48: 12000000 INFO @ Wed, 22 Jul 2020 13:40:48: 8000000 INFO @ Wed, 22 Jul 2020 13:40:54: 17000000 INFO @ Wed, 22 Jul 2020 13:40:56: 13000000 INFO @ Wed, 22 Jul 2020 13:40:56: 9000000 INFO @ Wed, 22 Jul 2020 13:41:02: 18000000 INFO @ Wed, 22 Jul 2020 13:41:03: 14000000 INFO @ Wed, 22 Jul 2020 13:41:04: 10000000 INFO @ Wed, 22 Jul 2020 13:41:10: 19000000 INFO @ Wed, 22 Jul 2020 13:41:11: 15000000 INFO @ Wed, 22 Jul 2020 13:41:12: 11000000 INFO @ Wed, 22 Jul 2020 13:41:18: 20000000 INFO @ Wed, 22 Jul 2020 13:41:19: 16000000 INFO @ Wed, 22 Jul 2020 13:41:20: 12000000 INFO @ Wed, 22 Jul 2020 13:41:25: 21000000 INFO @ Wed, 22 Jul 2020 13:41:27: 17000000 INFO @ Wed, 22 Jul 2020 13:41:28: 13000000 INFO @ Wed, 22 Jul 2020 13:41:33: 22000000 INFO @ Wed, 22 Jul 2020 13:41:34: 18000000 INFO @ Wed, 22 Jul 2020 13:41:35: 14000000 INFO @ Wed, 22 Jul 2020 13:41:41: 23000000 INFO @ Wed, 22 Jul 2020 13:41:42: 19000000 INFO @ Wed, 22 Jul 2020 13:41:43: 15000000 INFO @ Wed, 22 Jul 2020 13:41:48: 24000000 INFO @ Wed, 22 Jul 2020 13:41:50: 20000000 INFO @ Wed, 22 Jul 2020 13:41:51: 16000000 INFO @ Wed, 22 Jul 2020 13:41:56: 25000000 INFO @ Wed, 22 Jul 2020 13:41:58: 21000000 INFO @ Wed, 22 Jul 2020 13:41:58: 17000000 INFO @ Wed, 22 Jul 2020 13:42:03: 26000000 INFO @ Wed, 22 Jul 2020 13:42:05: 22000000 INFO @ Wed, 22 Jul 2020 13:42:06: 18000000 INFO @ Wed, 22 Jul 2020 13:42:11: 27000000 INFO @ Wed, 22 Jul 2020 13:42:13: 23000000 INFO @ Wed, 22 Jul 2020 13:42:14: 19000000 INFO @ Wed, 22 Jul 2020 13:42:18: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:42:21: 24000000 INFO @ Wed, 22 Jul 2020 13:42:21: 20000000 INFO @ Wed, 22 Jul 2020 13:42:25: 29000000 INFO @ Wed, 22 Jul 2020 13:42:28: 25000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:42:29: 21000000 INFO @ Wed, 22 Jul 2020 13:42:33: 30000000 INFO @ Wed, 22 Jul 2020 13:42:35: 26000000 INFO @ Wed, 22 Jul 2020 13:42:37: 22000000 INFO @ Wed, 22 Jul 2020 13:42:40: 31000000 INFO @ Wed, 22 Jul 2020 13:42:43: 27000000 INFO @ Wed, 22 Jul 2020 13:42:44: 23000000 INFO @ Wed, 22 Jul 2020 13:42:48: 32000000 INFO @ Wed, 22 Jul 2020 13:42:50: 28000000 INFO @ Wed, 22 Jul 2020 13:42:52: 24000000 INFO @ Wed, 22 Jul 2020 13:42:56: 33000000 INFO @ Wed, 22 Jul 2020 13:42:58: 29000000 INFO @ Wed, 22 Jul 2020 13:42:59: 25000000 INFO @ Wed, 22 Jul 2020 13:43:03: 34000000 INFO @ Wed, 22 Jul 2020 13:43:05: 30000000 INFO @ Wed, 22 Jul 2020 13:43:07: 26000000 INFO @ Wed, 22 Jul 2020 13:43:11: 35000000 INFO @ Wed, 22 Jul 2020 13:43:13: 31000000 INFO @ Wed, 22 Jul 2020 13:43:14: 27000000 INFO @ Wed, 22 Jul 2020 13:43:19: 36000000 INFO @ Wed, 22 Jul 2020 13:43:20: 32000000 INFO @ Wed, 22 Jul 2020 13:43:22: 28000000 INFO @ Wed, 22 Jul 2020 13:43:26: 37000000 INFO @ Wed, 22 Jul 2020 13:43:28: 33000000 INFO @ Wed, 22 Jul 2020 13:43:29: 29000000 INFO @ Wed, 22 Jul 2020 13:43:34: 38000000 INFO @ Wed, 22 Jul 2020 13:43:36: 34000000 INFO @ Wed, 22 Jul 2020 13:43:37: 30000000 INFO @ Wed, 22 Jul 2020 13:43:41: 39000000 INFO @ Wed, 22 Jul 2020 13:43:43: 35000000 INFO @ Wed, 22 Jul 2020 13:43:44: 31000000 INFO @ Wed, 22 Jul 2020 13:43:49: 40000000 INFO @ Wed, 22 Jul 2020 13:43:51: 36000000 INFO @ Wed, 22 Jul 2020 13:43:52: 32000000 INFO @ Wed, 22 Jul 2020 13:43:56: 41000000 INFO @ Wed, 22 Jul 2020 13:43:59: 37000000 INFO @ Wed, 22 Jul 2020 13:43:59: 33000000 INFO @ Wed, 22 Jul 2020 13:44:02: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:44:02: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:44:02: #1 total tags in treatment: 19122822 INFO @ Wed, 22 Jul 2020 13:44:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:44:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:44:03: #1 tags after filtering in treatment: 6776418 INFO @ Wed, 22 Jul 2020 13:44:03: #1 Redundant rate of treatment: 0.65 INFO @ Wed, 22 Jul 2020 13:44:03: #1 finished! INFO @ Wed, 22 Jul 2020 13:44:03: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:44:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:44:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:44:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:44:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:44:06: 38000000 INFO @ Wed, 22 Jul 2020 13:44:07: 34000000 INFO @ Wed, 22 Jul 2020 13:44:13: 39000000 INFO @ Wed, 22 Jul 2020 13:44:14: 35000000 INFO @ Wed, 22 Jul 2020 13:44:21: 40000000 INFO @ Wed, 22 Jul 2020 13:44:22: 36000000 INFO @ Wed, 22 Jul 2020 13:44:28: 41000000 INFO @ Wed, 22 Jul 2020 13:44:29: 37000000 INFO @ Wed, 22 Jul 2020 13:44:35: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:44:35: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:44:35: #1 total tags in treatment: 19122822 INFO @ Wed, 22 Jul 2020 13:44:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:44:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:44:35: #1 tags after filtering in treatment: 6776418 INFO @ Wed, 22 Jul 2020 13:44:35: #1 Redundant rate of treatment: 0.65 INFO @ Wed, 22 Jul 2020 13:44:35: #1 finished! INFO @ Wed, 22 Jul 2020 13:44:35: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:44:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:44:36: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:44:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:44:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:44:37: 38000000 INFO @ Wed, 22 Jul 2020 13:44:43: 39000000 INFO @ Wed, 22 Jul 2020 13:44:50: 40000000 INFO @ Wed, 22 Jul 2020 13:44:56: 41000000 INFO @ Wed, 22 Jul 2020 13:45:02: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:45:02: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:45:02: #1 total tags in treatment: 19122822 INFO @ Wed, 22 Jul 2020 13:45:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:45:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:45:02: #1 tags after filtering in treatment: 6776418 INFO @ Wed, 22 Jul 2020 13:45:02: #1 Redundant rate of treatment: 0.65 INFO @ Wed, 22 Jul 2020 13:45:02: #1 finished! INFO @ Wed, 22 Jul 2020 13:45:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:45:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:45:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:45:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:45:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911535/SRX6911535.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling