Job ID = 7104740 SRX = SRX6911526 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17263470 spots for SRR10191169/SRR10191169.sra Written 17263470 spots for SRR10191169/SRR10191169.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:16 17263470 reads; of these: 17263470 (100.00%) were paired; of these: 3484789 (20.19%) aligned concordantly 0 times 11028424 (63.88%) aligned concordantly exactly 1 time 2750257 (15.93%) aligned concordantly >1 times ---- 3484789 pairs aligned concordantly 0 times; of these: 1459615 (41.89%) aligned discordantly 1 time ---- 2025174 pairs aligned 0 times concordantly or discordantly; of these: 4050348 mates make up the pairs; of these: 2958294 (73.04%) aligned 0 times 441892 (10.91%) aligned exactly 1 time 650162 (16.05%) aligned >1 times 91.43% overall alignment rate Time searching: 00:19:16 Overall time: 00:19:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 570868 / 15230433 = 0.0375 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:01:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:01:58: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:01:58: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:02:06: 1000000 INFO @ Wed, 22 Jul 2020 13:02:13: 2000000 INFO @ Wed, 22 Jul 2020 13:02:20: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:02:28: 4000000 INFO @ Wed, 22 Jul 2020 13:02:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:02:28: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:02:28: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:02:36: 5000000 INFO @ Wed, 22 Jul 2020 13:02:39: 1000000 INFO @ Wed, 22 Jul 2020 13:02:46: 6000000 INFO @ Wed, 22 Jul 2020 13:02:50: 2000000 INFO @ Wed, 22 Jul 2020 13:02:55: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:02:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:02:58: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:02:58: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:03:01: 3000000 INFO @ Wed, 22 Jul 2020 13:03:04: 8000000 INFO @ Wed, 22 Jul 2020 13:03:08: 1000000 INFO @ Wed, 22 Jul 2020 13:03:12: 4000000 INFO @ Wed, 22 Jul 2020 13:03:14: 9000000 INFO @ Wed, 22 Jul 2020 13:03:18: 2000000 INFO @ Wed, 22 Jul 2020 13:03:23: 5000000 INFO @ Wed, 22 Jul 2020 13:03:24: 10000000 INFO @ Wed, 22 Jul 2020 13:03:28: 3000000 INFO @ Wed, 22 Jul 2020 13:03:34: 11000000 INFO @ Wed, 22 Jul 2020 13:03:35: 6000000 INFO @ Wed, 22 Jul 2020 13:03:38: 4000000 INFO @ Wed, 22 Jul 2020 13:03:44: 12000000 INFO @ Wed, 22 Jul 2020 13:03:46: 7000000 INFO @ Wed, 22 Jul 2020 13:03:48: 5000000 INFO @ Wed, 22 Jul 2020 13:03:54: 13000000 INFO @ Wed, 22 Jul 2020 13:03:57: 8000000 INFO @ Wed, 22 Jul 2020 13:03:57: 6000000 INFO @ Wed, 22 Jul 2020 13:04:04: 14000000 INFO @ Wed, 22 Jul 2020 13:04:07: 7000000 INFO @ Wed, 22 Jul 2020 13:04:08: 9000000 INFO @ Wed, 22 Jul 2020 13:04:14: 15000000 INFO @ Wed, 22 Jul 2020 13:04:17: 8000000 INFO @ Wed, 22 Jul 2020 13:04:19: 10000000 INFO @ Wed, 22 Jul 2020 13:04:24: 16000000 INFO @ Wed, 22 Jul 2020 13:04:27: 9000000 INFO @ Wed, 22 Jul 2020 13:04:30: 11000000 INFO @ Wed, 22 Jul 2020 13:04:34: 17000000 INFO @ Wed, 22 Jul 2020 13:04:37: 10000000 INFO @ Wed, 22 Jul 2020 13:04:41: 12000000 INFO @ Wed, 22 Jul 2020 13:04:43: 18000000 INFO @ Wed, 22 Jul 2020 13:04:47: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:04:52: 13000000 INFO @ Wed, 22 Jul 2020 13:04:53: 19000000 INFO @ Wed, 22 Jul 2020 13:04:57: 12000000 INFO @ Wed, 22 Jul 2020 13:05:03: 14000000 INFO @ Wed, 22 Jul 2020 13:05:03: 20000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:05:07: 13000000 INFO @ Wed, 22 Jul 2020 13:05:13: 21000000 INFO @ Wed, 22 Jul 2020 13:05:13: 15000000 INFO @ Wed, 22 Jul 2020 13:05:17: 14000000 INFO @ Wed, 22 Jul 2020 13:05:23: 22000000 INFO @ Wed, 22 Jul 2020 13:05:24: 16000000 INFO @ Wed, 22 Jul 2020 13:05:27: 15000000 INFO @ Wed, 22 Jul 2020 13:05:33: 23000000 INFO @ Wed, 22 Jul 2020 13:05:34: 17000000 INFO @ Wed, 22 Jul 2020 13:05:37: 16000000 INFO @ Wed, 22 Jul 2020 13:05:43: 24000000 INFO @ Wed, 22 Jul 2020 13:05:45: 18000000 INFO @ Wed, 22 Jul 2020 13:05:47: 17000000 INFO @ Wed, 22 Jul 2020 13:05:53: 25000000 INFO @ Wed, 22 Jul 2020 13:05:56: 19000000 INFO @ Wed, 22 Jul 2020 13:05:57: 18000000 INFO @ Wed, 22 Jul 2020 13:06:03: 26000000 INFO @ Wed, 22 Jul 2020 13:06:06: 20000000 INFO @ Wed, 22 Jul 2020 13:06:06: 19000000 INFO @ Wed, 22 Jul 2020 13:06:13: 27000000 INFO @ Wed, 22 Jul 2020 13:06:16: 20000000 INFO @ Wed, 22 Jul 2020 13:06:17: 21000000 INFO @ Wed, 22 Jul 2020 13:06:22: 28000000 INFO @ Wed, 22 Jul 2020 13:06:26: 21000000 INFO @ Wed, 22 Jul 2020 13:06:28: 22000000 INFO @ Wed, 22 Jul 2020 13:06:32: 29000000 INFO @ Wed, 22 Jul 2020 13:06:36: 22000000 INFO @ Wed, 22 Jul 2020 13:06:38: 23000000 INFO @ Wed, 22 Jul 2020 13:06:42: 30000000 INFO @ Wed, 22 Jul 2020 13:06:46: 23000000 INFO @ Wed, 22 Jul 2020 13:06:46: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:06:46: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:06:46: #1 total tags in treatment: 13212017 INFO @ Wed, 22 Jul 2020 13:06:46: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:06:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:06:47: #1 tags after filtering in treatment: 8479586 INFO @ Wed, 22 Jul 2020 13:06:47: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:06:47: #1 finished! INFO @ Wed, 22 Jul 2020 13:06:47: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:06:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:06:47: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:06:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:06:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:06:49: 24000000 INFO @ Wed, 22 Jul 2020 13:06:56: 24000000 INFO @ Wed, 22 Jul 2020 13:07:00: 25000000 INFO @ Wed, 22 Jul 2020 13:07:05: 25000000 INFO @ Wed, 22 Jul 2020 13:07:11: 26000000 INFO @ Wed, 22 Jul 2020 13:07:15: 26000000 INFO @ Wed, 22 Jul 2020 13:07:21: 27000000 INFO @ Wed, 22 Jul 2020 13:07:24: 27000000 INFO @ Wed, 22 Jul 2020 13:07:32: 28000000 INFO @ Wed, 22 Jul 2020 13:07:34: 28000000 INFO @ Wed, 22 Jul 2020 13:07:42: 29000000 INFO @ Wed, 22 Jul 2020 13:07:44: 29000000 INFO @ Wed, 22 Jul 2020 13:07:53: 30000000 INFO @ Wed, 22 Jul 2020 13:07:54: 30000000 INFO @ Wed, 22 Jul 2020 13:07:58: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:07:58: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:07:58: #1 total tags in treatment: 13212017 INFO @ Wed, 22 Jul 2020 13:07:58: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:07:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:07:58: #1 tags after filtering in treatment: 8479586 INFO @ Wed, 22 Jul 2020 13:07:58: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:07:58: #1 finished! INFO @ Wed, 22 Jul 2020 13:07:58: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:07:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:07:58: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:07:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10_peaks.narrowPeak: No such file or directory INFO @ Wed, 22 Jul 2020 13:07:58: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:07:58: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:07:58: #1 total tags in treatment: 13212017 INFO @ Wed, 22 Jul 2020 13:07:58: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:07:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:07:59: #1 tags after filtering in treatment: 8479586 INFO @ Wed, 22 Jul 2020 13:07:59: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 13:07:59: #1 finished! INFO @ Wed, 22 Jul 2020 13:07:59: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:07:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:07:59: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:07:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:07:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911526/SRX6911526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling