Job ID = 7103444 SRX = SRX6911524 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17718861 spots for SRR10191167/SRR10191167.sra Written 17718861 spots for SRR10191167/SRR10191167.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:51 17718861 reads; of these: 17718861 (100.00%) were paired; of these: 5944837 (33.55%) aligned concordantly 0 times 7351759 (41.49%) aligned concordantly exactly 1 time 4422265 (24.96%) aligned concordantly >1 times ---- 5944837 pairs aligned concordantly 0 times; of these: 308445 (5.19%) aligned discordantly 1 time ---- 5636392 pairs aligned 0 times concordantly or discordantly; of these: 11272784 mates make up the pairs; of these: 10617710 (94.19%) aligned 0 times 288005 (2.55%) aligned exactly 1 time 367069 (3.26%) aligned >1 times 70.04% overall alignment rate Time searching: 00:15:52 Overall time: 00:15:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2674517 / 12079710 = 0.2214 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:47:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:47:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:47:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:48:00: 1000000 INFO @ Wed, 22 Jul 2020 12:48:06: 2000000 INFO @ Wed, 22 Jul 2020 12:48:12: 3000000 INFO @ Wed, 22 Jul 2020 12:48:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:48:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:48:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:48:24: 5000000 INFO @ Wed, 22 Jul 2020 12:48:30: 6000000 INFO @ Wed, 22 Jul 2020 12:48:30: 1000000 INFO @ Wed, 22 Jul 2020 12:48:35: 7000000 INFO @ Wed, 22 Jul 2020 12:48:36: 2000000 INFO @ Wed, 22 Jul 2020 12:48:41: 8000000 INFO @ Wed, 22 Jul 2020 12:48:43: 3000000 INFO @ Wed, 22 Jul 2020 12:48:47: 9000000 INFO @ Wed, 22 Jul 2020 12:48:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:48:53: 10000000 INFO @ Wed, 22 Jul 2020 12:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:48:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:48:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:48:55: 5000000 INFO @ Wed, 22 Jul 2020 12:48:59: 11000000 INFO @ Wed, 22 Jul 2020 12:49:00: 1000000 INFO @ Wed, 22 Jul 2020 12:49:00: 6000000 INFO @ Wed, 22 Jul 2020 12:49:06: 12000000 INFO @ Wed, 22 Jul 2020 12:49:06: 7000000 INFO @ Wed, 22 Jul 2020 12:49:06: 2000000 INFO @ Wed, 22 Jul 2020 12:49:12: 13000000 INFO @ Wed, 22 Jul 2020 12:49:12: 8000000 INFO @ Wed, 22 Jul 2020 12:49:13: 3000000 INFO @ Wed, 22 Jul 2020 12:49:18: 14000000 INFO @ Wed, 22 Jul 2020 12:49:18: 9000000 INFO @ Wed, 22 Jul 2020 12:49:19: 4000000 INFO @ Wed, 22 Jul 2020 12:49:24: 15000000 INFO @ Wed, 22 Jul 2020 12:49:24: 10000000 INFO @ Wed, 22 Jul 2020 12:49:25: 5000000 INFO @ Wed, 22 Jul 2020 12:49:30: 16000000 INFO @ Wed, 22 Jul 2020 12:49:30: 6000000 INFO @ Wed, 22 Jul 2020 12:49:30: 11000000 INFO @ Wed, 22 Jul 2020 12:49:35: 17000000 INFO @ Wed, 22 Jul 2020 12:49:36: 7000000 INFO @ Wed, 22 Jul 2020 12:49:37: 12000000 INFO @ Wed, 22 Jul 2020 12:49:41: 18000000 INFO @ Wed, 22 Jul 2020 12:49:42: 8000000 INFO @ Wed, 22 Jul 2020 12:49:43: 13000000 INFO @ Wed, 22 Jul 2020 12:49:48: 19000000 INFO @ Wed, 22 Jul 2020 12:49:48: 9000000 INFO @ Wed, 22 Jul 2020 12:49:49: 14000000 INFO @ Wed, 22 Jul 2020 12:49:51: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:49:51: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:49:51: #1 total tags in treatment: 9121950 INFO @ Wed, 22 Jul 2020 12:49:51: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:49:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:49:51: #1 tags after filtering in treatment: 5713350 INFO @ Wed, 22 Jul 2020 12:49:51: #1 Redundant rate of treatment: 0.37 INFO @ Wed, 22 Jul 2020 12:49:51: #1 finished! INFO @ Wed, 22 Jul 2020 12:49:51: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:49:51: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:49:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:49:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:49:54: 10000000 INFO @ Wed, 22 Jul 2020 12:49:55: 15000000 INFO @ Wed, 22 Jul 2020 12:50:00: 11000000 INFO @ Wed, 22 Jul 2020 12:50:01: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:50:07: 17000000 INFO @ Wed, 22 Jul 2020 12:50:07: 12000000 INFO @ Wed, 22 Jul 2020 12:50:13: 18000000 INFO @ Wed, 22 Jul 2020 12:50:13: 13000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:50:19: 19000000 INFO @ Wed, 22 Jul 2020 12:50:19: 14000000 INFO @ Wed, 22 Jul 2020 12:50:22: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:50:22: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:50:22: #1 total tags in treatment: 9121950 INFO @ Wed, 22 Jul 2020 12:50:22: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:50:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:50:22: #1 tags after filtering in treatment: 5713350 INFO @ Wed, 22 Jul 2020 12:50:22: #1 Redundant rate of treatment: 0.37 INFO @ Wed, 22 Jul 2020 12:50:22: #1 finished! INFO @ Wed, 22 Jul 2020 12:50:22: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:50:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:50:23: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:50:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:50:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:50:25: 15000000 INFO @ Wed, 22 Jul 2020 12:50:31: 16000000 INFO @ Wed, 22 Jul 2020 12:50:37: 17000000 INFO @ Wed, 22 Jul 2020 12:50:43: 18000000 INFO @ Wed, 22 Jul 2020 12:50:49: 19000000 INFO @ Wed, 22 Jul 2020 12:50:53: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:50:53: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:50:53: #1 total tags in treatment: 9121950 INFO @ Wed, 22 Jul 2020 12:50:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:50:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:50:53: #1 tags after filtering in treatment: 5713350 INFO @ Wed, 22 Jul 2020 12:50:53: #1 Redundant rate of treatment: 0.37 INFO @ Wed, 22 Jul 2020 12:50:53: #1 finished! INFO @ Wed, 22 Jul 2020 12:50:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:50:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:50:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:50:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:50:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911524/SRX6911524.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling