Job ID = 7100231 SRX = SRX6911514 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14029904 spots for SRR10191157/SRR10191157.sra Written 14029904 spots for SRR10191157/SRR10191157.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:10 14029904 reads; of these: 14029904 (100.00%) were paired; of these: 2252796 (16.06%) aligned concordantly 0 times 10307710 (73.47%) aligned concordantly exactly 1 time 1469398 (10.47%) aligned concordantly >1 times ---- 2252796 pairs aligned concordantly 0 times; of these: 744096 (33.03%) aligned discordantly 1 time ---- 1508700 pairs aligned 0 times concordantly or discordantly; of these: 3017400 mates make up the pairs; of these: 1947708 (64.55%) aligned 0 times 755031 (25.02%) aligned exactly 1 time 314661 (10.43%) aligned >1 times 93.06% overall alignment rate Time searching: 00:15:10 Overall time: 00:15:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1047558 / 12519066 = 0.0837 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:38:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:38:36: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:38:36: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:38:43: 1000000 INFO @ Wed, 22 Jul 2020 12:38:50: 2000000 INFO @ Wed, 22 Jul 2020 12:38:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:39:05: 4000000 INFO @ Wed, 22 Jul 2020 12:39:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:39:05: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:39:05: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:39:13: 5000000 INFO @ Wed, 22 Jul 2020 12:39:14: 1000000 INFO @ Wed, 22 Jul 2020 12:39:22: 6000000 INFO @ Wed, 22 Jul 2020 12:39:22: 2000000 INFO @ Wed, 22 Jul 2020 12:39:30: 7000000 INFO @ Wed, 22 Jul 2020 12:39:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:39:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:39:36: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:39:36: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:39:38: 8000000 INFO @ Wed, 22 Jul 2020 12:39:39: 4000000 INFO @ Wed, 22 Jul 2020 12:39:44: 1000000 INFO @ Wed, 22 Jul 2020 12:39:46: 9000000 INFO @ Wed, 22 Jul 2020 12:39:47: 5000000 INFO @ Wed, 22 Jul 2020 12:39:52: 2000000 INFO @ Wed, 22 Jul 2020 12:39:55: 10000000 INFO @ Wed, 22 Jul 2020 12:39:56: 6000000 INFO @ Wed, 22 Jul 2020 12:40:01: 3000000 INFO @ Wed, 22 Jul 2020 12:40:03: 11000000 INFO @ Wed, 22 Jul 2020 12:40:05: 7000000 INFO @ Wed, 22 Jul 2020 12:40:09: 4000000 INFO @ Wed, 22 Jul 2020 12:40:12: 12000000 INFO @ Wed, 22 Jul 2020 12:40:14: 8000000 INFO @ Wed, 22 Jul 2020 12:40:18: 5000000 INFO @ Wed, 22 Jul 2020 12:40:20: 13000000 INFO @ Wed, 22 Jul 2020 12:40:22: 9000000 INFO @ Wed, 22 Jul 2020 12:40:26: 6000000 INFO @ Wed, 22 Jul 2020 12:40:28: 14000000 INFO @ Wed, 22 Jul 2020 12:40:31: 10000000 INFO @ Wed, 22 Jul 2020 12:40:34: 7000000 INFO @ Wed, 22 Jul 2020 12:40:37: 15000000 INFO @ Wed, 22 Jul 2020 12:40:39: 11000000 INFO @ Wed, 22 Jul 2020 12:40:42: 8000000 INFO @ Wed, 22 Jul 2020 12:40:45: 16000000 INFO @ Wed, 22 Jul 2020 12:40:48: 12000000 INFO @ Wed, 22 Jul 2020 12:40:50: 9000000 INFO @ Wed, 22 Jul 2020 12:40:53: 17000000 INFO @ Wed, 22 Jul 2020 12:40:56: 13000000 INFO @ Wed, 22 Jul 2020 12:40:58: 10000000 INFO @ Wed, 22 Jul 2020 12:41:01: 18000000 INFO @ Wed, 22 Jul 2020 12:41:05: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:41:07: 11000000 INFO @ Wed, 22 Jul 2020 12:41:10: 19000000 INFO @ Wed, 22 Jul 2020 12:41:14: 15000000 INFO @ Wed, 22 Jul 2020 12:41:15: 12000000 INFO @ Wed, 22 Jul 2020 12:41:18: 20000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:41:22: 16000000 INFO @ Wed, 22 Jul 2020 12:41:24: 13000000 INFO @ Wed, 22 Jul 2020 12:41:27: 21000000 INFO @ Wed, 22 Jul 2020 12:41:31: 17000000 INFO @ Wed, 22 Jul 2020 12:41:32: 14000000 INFO @ Wed, 22 Jul 2020 12:41:35: 22000000 INFO @ Wed, 22 Jul 2020 12:41:40: 18000000 INFO @ Wed, 22 Jul 2020 12:41:41: 15000000 INFO @ Wed, 22 Jul 2020 12:41:44: 23000000 INFO @ Wed, 22 Jul 2020 12:41:49: 19000000 INFO @ Wed, 22 Jul 2020 12:41:49: 16000000 INFO @ Wed, 22 Jul 2020 12:41:52: 24000000 INFO @ Wed, 22 Jul 2020 12:41:52: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:41:52: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:41:52: #1 total tags in treatment: 10763091 INFO @ Wed, 22 Jul 2020 12:41:52: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:41:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:41:52: #1 tags after filtering in treatment: 6282935 INFO @ Wed, 22 Jul 2020 12:41:52: #1 Redundant rate of treatment: 0.42 INFO @ Wed, 22 Jul 2020 12:41:52: #1 finished! INFO @ Wed, 22 Jul 2020 12:41:52: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:41:52: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:41:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:41:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:41:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:41:57: 20000000 INFO @ Wed, 22 Jul 2020 12:41:57: 17000000 INFO @ Wed, 22 Jul 2020 12:42:06: 18000000 INFO @ Wed, 22 Jul 2020 12:42:06: 21000000 INFO @ Wed, 22 Jul 2020 12:42:14: 19000000 INFO @ Wed, 22 Jul 2020 12:42:14: 22000000 INFO @ Wed, 22 Jul 2020 12:42:22: 20000000 INFO @ Wed, 22 Jul 2020 12:42:23: 23000000 INFO @ Wed, 22 Jul 2020 12:42:30: 21000000 INFO @ Wed, 22 Jul 2020 12:42:31: 24000000 INFO @ Wed, 22 Jul 2020 12:42:31: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:42:31: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:42:31: #1 total tags in treatment: 10763091 INFO @ Wed, 22 Jul 2020 12:42:31: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:42:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:42:32: #1 tags after filtering in treatment: 6282935 INFO @ Wed, 22 Jul 2020 12:42:32: #1 Redundant rate of treatment: 0.42 INFO @ Wed, 22 Jul 2020 12:42:32: #1 finished! INFO @ Wed, 22 Jul 2020 12:42:32: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:42:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:42:32: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:42:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:42:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:42:38: 22000000 INFO @ Wed, 22 Jul 2020 12:42:45: 23000000 INFO @ Wed, 22 Jul 2020 12:42:52: 24000000 INFO @ Wed, 22 Jul 2020 12:42:53: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:42:53: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:42:53: #1 total tags in treatment: 10763091 INFO @ Wed, 22 Jul 2020 12:42:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:42:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:42:53: #1 tags after filtering in treatment: 6282935 INFO @ Wed, 22 Jul 2020 12:42:53: #1 Redundant rate of treatment: 0.42 INFO @ Wed, 22 Jul 2020 12:42:53: #1 finished! INFO @ Wed, 22 Jul 2020 12:42:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:42:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:42:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:42:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:42:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911514/SRX6911514.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling