Job ID = 7099906 SRX = SRX6911511 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30303041 spots for SRR10191138/SRR10191138.sra Written 30303041 spots for SRR10191138/SRR10191138.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:14 30303041 reads; of these: 30303041 (100.00%) were paired; of these: 5267166 (17.38%) aligned concordantly 0 times 20722482 (68.38%) aligned concordantly exactly 1 time 4313393 (14.23%) aligned concordantly >1 times ---- 5267166 pairs aligned concordantly 0 times; of these: 1766491 (33.54%) aligned discordantly 1 time ---- 3500675 pairs aligned 0 times concordantly or discordantly; of these: 7001350 mates make up the pairs; of these: 4415645 (63.07%) aligned 0 times 1604552 (22.92%) aligned exactly 1 time 981153 (14.01%) aligned >1 times 92.71% overall alignment rate Time searching: 00:34:14 Overall time: 00:34:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2070844 / 26786634 = 0.0773 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:04:38: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:04:38: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:04:44: 1000000 INFO @ Wed, 22 Jul 2020 13:04:50: 2000000 INFO @ Wed, 22 Jul 2020 13:04:56: 3000000 INFO @ Wed, 22 Jul 2020 13:05:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:05:08: 5000000 INFO @ Wed, 22 Jul 2020 13:05:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:05:08: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:05:08: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:05:14: 6000000 INFO @ Wed, 22 Jul 2020 13:05:14: 1000000 INFO @ Wed, 22 Jul 2020 13:05:20: 7000000 INFO @ Wed, 22 Jul 2020 13:05:21: 2000000 INFO @ Wed, 22 Jul 2020 13:05:27: 8000000 INFO @ Wed, 22 Jul 2020 13:05:27: 3000000 INFO @ Wed, 22 Jul 2020 13:05:33: 9000000 INFO @ Wed, 22 Jul 2020 13:05:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:05:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:05:38: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:05:38: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:05:39: 10000000 INFO @ Wed, 22 Jul 2020 13:05:39: 5000000 INFO @ Wed, 22 Jul 2020 13:05:45: 1000000 INFO @ Wed, 22 Jul 2020 13:05:45: 11000000 INFO @ Wed, 22 Jul 2020 13:05:46: 6000000 INFO @ Wed, 22 Jul 2020 13:05:51: 2000000 INFO @ Wed, 22 Jul 2020 13:05:52: 12000000 INFO @ Wed, 22 Jul 2020 13:05:52: 7000000 INFO @ Wed, 22 Jul 2020 13:05:57: 3000000 INFO @ Wed, 22 Jul 2020 13:05:58: 13000000 INFO @ Wed, 22 Jul 2020 13:05:58: 8000000 INFO @ Wed, 22 Jul 2020 13:06:04: 4000000 INFO @ Wed, 22 Jul 2020 13:06:04: 14000000 INFO @ Wed, 22 Jul 2020 13:06:05: 9000000 INFO @ Wed, 22 Jul 2020 13:06:10: 15000000 INFO @ Wed, 22 Jul 2020 13:06:11: 5000000 INFO @ Wed, 22 Jul 2020 13:06:11: 10000000 INFO @ Wed, 22 Jul 2020 13:06:17: 16000000 INFO @ Wed, 22 Jul 2020 13:06:17: 6000000 INFO @ Wed, 22 Jul 2020 13:06:18: 11000000 INFO @ Wed, 22 Jul 2020 13:06:23: 17000000 INFO @ Wed, 22 Jul 2020 13:06:23: 7000000 INFO @ Wed, 22 Jul 2020 13:06:24: 12000000 INFO @ Wed, 22 Jul 2020 13:06:29: 18000000 INFO @ Wed, 22 Jul 2020 13:06:30: 8000000 INFO @ Wed, 22 Jul 2020 13:06:31: 13000000 INFO @ Wed, 22 Jul 2020 13:06:36: 19000000 INFO @ Wed, 22 Jul 2020 13:06:36: 9000000 INFO @ Wed, 22 Jul 2020 13:06:37: 14000000 INFO @ Wed, 22 Jul 2020 13:06:42: 20000000 INFO @ Wed, 22 Jul 2020 13:06:43: 10000000 INFO @ Wed, 22 Jul 2020 13:06:43: 15000000 INFO @ Wed, 22 Jul 2020 13:06:49: 21000000 INFO @ Wed, 22 Jul 2020 13:06:49: 11000000 INFO @ Wed, 22 Jul 2020 13:06:49: 16000000 INFO @ Wed, 22 Jul 2020 13:06:55: 22000000 INFO @ Wed, 22 Jul 2020 13:06:55: 12000000 INFO @ Wed, 22 Jul 2020 13:06:56: 17000000 INFO @ Wed, 22 Jul 2020 13:07:02: 13000000 INFO @ Wed, 22 Jul 2020 13:07:02: 23000000 INFO @ Wed, 22 Jul 2020 13:07:02: 18000000 INFO @ Wed, 22 Jul 2020 13:07:08: 14000000 INFO @ Wed, 22 Jul 2020 13:07:08: 19000000 INFO @ Wed, 22 Jul 2020 13:07:09: 24000000 INFO @ Wed, 22 Jul 2020 13:07:14: 15000000 INFO @ Wed, 22 Jul 2020 13:07:15: 20000000 INFO @ Wed, 22 Jul 2020 13:07:15: 25000000 INFO @ Wed, 22 Jul 2020 13:07:21: 16000000 INFO @ Wed, 22 Jul 2020 13:07:21: 21000000 INFO @ Wed, 22 Jul 2020 13:07:21: 26000000 INFO @ Wed, 22 Jul 2020 13:07:27: 17000000 INFO @ Wed, 22 Jul 2020 13:07:28: 27000000 INFO @ Wed, 22 Jul 2020 13:07:28: 22000000 INFO @ Wed, 22 Jul 2020 13:07:33: 18000000 INFO @ Wed, 22 Jul 2020 13:07:34: 28000000 INFO @ Wed, 22 Jul 2020 13:07:34: 23000000 INFO @ Wed, 22 Jul 2020 13:07:40: 19000000 INFO @ Wed, 22 Jul 2020 13:07:40: 29000000 INFO @ Wed, 22 Jul 2020 13:07:40: 24000000 INFO @ Wed, 22 Jul 2020 13:07:46: 20000000 INFO @ Wed, 22 Jul 2020 13:07:46: 30000000 INFO @ Wed, 22 Jul 2020 13:07:47: 25000000 INFO @ Wed, 22 Jul 2020 13:07:52: 21000000 INFO @ Wed, 22 Jul 2020 13:07:53: 31000000 INFO @ Wed, 22 Jul 2020 13:07:53: 26000000 INFO @ Wed, 22 Jul 2020 13:07:58: 22000000 INFO @ Wed, 22 Jul 2020 13:07:59: 32000000 INFO @ Wed, 22 Jul 2020 13:07:59: 27000000 INFO @ Wed, 22 Jul 2020 13:08:05: 23000000 INFO @ Wed, 22 Jul 2020 13:08:05: 33000000 INFO @ Wed, 22 Jul 2020 13:08:05: 28000000 INFO @ Wed, 22 Jul 2020 13:08:11: 24000000 INFO @ Wed, 22 Jul 2020 13:08:11: 34000000 INFO @ Wed, 22 Jul 2020 13:08:12: 29000000 INFO @ Wed, 22 Jul 2020 13:08:17: 25000000 INFO @ Wed, 22 Jul 2020 13:08:17: 35000000 INFO @ Wed, 22 Jul 2020 13:08:18: 30000000 INFO @ Wed, 22 Jul 2020 13:08:23: 36000000 INFO @ Wed, 22 Jul 2020 13:08:23: 26000000 INFO @ Wed, 22 Jul 2020 13:08:24: 31000000 INFO @ Wed, 22 Jul 2020 13:08:29: 37000000 INFO @ Wed, 22 Jul 2020 13:08:30: 27000000 INFO @ Wed, 22 Jul 2020 13:08:30: 32000000 INFO @ Wed, 22 Jul 2020 13:08:36: 38000000 INFO @ Wed, 22 Jul 2020 13:08:36: 28000000 INFO @ Wed, 22 Jul 2020 13:08:36: 33000000 INFO @ Wed, 22 Jul 2020 13:08:42: 39000000 INFO @ Wed, 22 Jul 2020 13:08:42: 29000000 INFO @ Wed, 22 Jul 2020 13:08:42: 34000000 INFO @ Wed, 22 Jul 2020 13:08:48: 40000000 INFO @ Wed, 22 Jul 2020 13:08:49: 30000000 INFO @ Wed, 22 Jul 2020 13:08:49: 35000000 INFO @ Wed, 22 Jul 2020 13:08:55: 41000000 INFO @ Wed, 22 Jul 2020 13:08:55: 36000000 INFO @ Wed, 22 Jul 2020 13:08:55: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:09:01: 37000000 INFO @ Wed, 22 Jul 2020 13:09:01: 42000000 INFO @ Wed, 22 Jul 2020 13:09:01: 32000000 INFO @ Wed, 22 Jul 2020 13:09:07: 38000000 INFO @ Wed, 22 Jul 2020 13:09:07: 43000000 INFO @ Wed, 22 Jul 2020 13:09:07: 33000000 INFO @ Wed, 22 Jul 2020 13:09:13: 39000000 INFO @ Wed, 22 Jul 2020 13:09:13: 34000000 INFO @ Wed, 22 Jul 2020 13:09:13: 44000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:09:20: 40000000 INFO @ Wed, 22 Jul 2020 13:09:20: 35000000 INFO @ Wed, 22 Jul 2020 13:09:20: 45000000 INFO @ Wed, 22 Jul 2020 13:09:26: 36000000 INFO @ Wed, 22 Jul 2020 13:09:26: 41000000 INFO @ Wed, 22 Jul 2020 13:09:26: 46000000 INFO @ Wed, 22 Jul 2020 13:09:32: 37000000 INFO @ Wed, 22 Jul 2020 13:09:32: 42000000 INFO @ Wed, 22 Jul 2020 13:09:32: 47000000 INFO @ Wed, 22 Jul 2020 13:09:38: 38000000 INFO @ Wed, 22 Jul 2020 13:09:38: 43000000 INFO @ Wed, 22 Jul 2020 13:09:39: 48000000 INFO @ Wed, 22 Jul 2020 13:09:45: 39000000 INFO @ Wed, 22 Jul 2020 13:09:45: 44000000 INFO @ Wed, 22 Jul 2020 13:09:45: 49000000 INFO @ Wed, 22 Jul 2020 13:09:51: 40000000 INFO @ Wed, 22 Jul 2020 13:09:51: 45000000 INFO @ Wed, 22 Jul 2020 13:09:51: 50000000 INFO @ Wed, 22 Jul 2020 13:09:57: 41000000 INFO @ Wed, 22 Jul 2020 13:09:57: 46000000 INFO @ Wed, 22 Jul 2020 13:09:58: 51000000 INFO @ Wed, 22 Jul 2020 13:10:04: 42000000 INFO @ Wed, 22 Jul 2020 13:10:04: 47000000 INFO @ Wed, 22 Jul 2020 13:10:04: 52000000 INFO @ Wed, 22 Jul 2020 13:10:05: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:10:05: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:10:05: #1 total tags in treatment: 22989103 INFO @ Wed, 22 Jul 2020 13:10:05: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:10:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:10:05: #1 tags after filtering in treatment: 12215572 INFO @ Wed, 22 Jul 2020 13:10:05: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 13:10:05: #1 finished! INFO @ Wed, 22 Jul 2020 13:10:05: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:10:06: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:10:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:10:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:10:10: 43000000 INFO @ Wed, 22 Jul 2020 13:10:10: 48000000 INFO @ Wed, 22 Jul 2020 13:10:16: 44000000 INFO @ Wed, 22 Jul 2020 13:10:16: 49000000 INFO @ Wed, 22 Jul 2020 13:10:22: 45000000 INFO @ Wed, 22 Jul 2020 13:10:22: 50000000 INFO @ Wed, 22 Jul 2020 13:10:29: 46000000 INFO @ Wed, 22 Jul 2020 13:10:29: 51000000 INFO @ Wed, 22 Jul 2020 13:10:35: 47000000 INFO @ Wed, 22 Jul 2020 13:10:35: 52000000 INFO @ Wed, 22 Jul 2020 13:10:35: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:10:35: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:10:35: #1 total tags in treatment: 22989103 INFO @ Wed, 22 Jul 2020 13:10:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:10:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:10:36: #1 tags after filtering in treatment: 12215572 INFO @ Wed, 22 Jul 2020 13:10:36: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 13:10:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:10:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:10:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:10:37: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:10:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:10:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:10:41: 48000000 INFO @ Wed, 22 Jul 2020 13:10:47: 49000000 INFO @ Wed, 22 Jul 2020 13:10:53: 50000000 INFO @ Wed, 22 Jul 2020 13:10:59: 51000000 INFO @ Wed, 22 Jul 2020 13:11:04: 52000000 INFO @ Wed, 22 Jul 2020 13:11:05: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:11:05: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:11:05: #1 total tags in treatment: 22989103 INFO @ Wed, 22 Jul 2020 13:11:05: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:11:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:11:06: #1 tags after filtering in treatment: 12215572 INFO @ Wed, 22 Jul 2020 13:11:06: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 13:11:06: #1 finished! INFO @ Wed, 22 Jul 2020 13:11:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:11:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:11:06: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:11:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:11:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911511/SRX6911511.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling