Job ID = 7099103 SRX = SRX6911497 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 44038798 spots for SRR10191124/SRR10191124.sra Written 44038798 spots for SRR10191124/SRR10191124.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:33 44038798 reads; of these: 44038798 (100.00%) were paired; of these: 21624001 (49.10%) aligned concordantly 0 times 14554204 (33.05%) aligned concordantly exactly 1 time 7860593 (17.85%) aligned concordantly >1 times ---- 21624001 pairs aligned concordantly 0 times; of these: 1221289 (5.65%) aligned discordantly 1 time ---- 20402712 pairs aligned 0 times concordantly or discordantly; of these: 40805424 mates make up the pairs; of these: 38069793 (93.30%) aligned 0 times 1138556 (2.79%) aligned exactly 1 time 1597075 (3.91%) aligned >1 times 56.78% overall alignment rate Time searching: 00:41:33 Overall time: 00:41:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12246539 / 23629392 = 0.5183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:04:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:04:01: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:04:01: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:04:09: 1000000 INFO @ Wed, 22 Jul 2020 13:04:16: 2000000 INFO @ Wed, 22 Jul 2020 13:04:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:04:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:04:31: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:04:31: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:04:32: 4000000 INFO @ Wed, 22 Jul 2020 13:04:40: 1000000 INFO @ Wed, 22 Jul 2020 13:04:40: 5000000 INFO @ Wed, 22 Jul 2020 13:04:49: 2000000 INFO @ Wed, 22 Jul 2020 13:04:49: 6000000 INFO @ Wed, 22 Jul 2020 13:04:57: 3000000 INFO @ Wed, 22 Jul 2020 13:04:57: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:05:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:05:01: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:05:01: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:05:06: 4000000 INFO @ Wed, 22 Jul 2020 13:05:06: 8000000 INFO @ Wed, 22 Jul 2020 13:05:10: 1000000 INFO @ Wed, 22 Jul 2020 13:05:15: 5000000 INFO @ Wed, 22 Jul 2020 13:05:15: 9000000 INFO @ Wed, 22 Jul 2020 13:05:19: 2000000 INFO @ Wed, 22 Jul 2020 13:05:23: 6000000 INFO @ Wed, 22 Jul 2020 13:05:23: 10000000 INFO @ Wed, 22 Jul 2020 13:05:27: 3000000 INFO @ Wed, 22 Jul 2020 13:05:32: 7000000 INFO @ Wed, 22 Jul 2020 13:05:32: 11000000 INFO @ Wed, 22 Jul 2020 13:05:36: 4000000 INFO @ Wed, 22 Jul 2020 13:05:40: 8000000 INFO @ Wed, 22 Jul 2020 13:05:40: 12000000 INFO @ Wed, 22 Jul 2020 13:05:44: 5000000 INFO @ Wed, 22 Jul 2020 13:05:49: 13000000 INFO @ Wed, 22 Jul 2020 13:05:49: 9000000 INFO @ Wed, 22 Jul 2020 13:05:53: 6000000 INFO @ Wed, 22 Jul 2020 13:05:57: 14000000 INFO @ Wed, 22 Jul 2020 13:05:57: 10000000 INFO @ Wed, 22 Jul 2020 13:06:01: 7000000 INFO @ Wed, 22 Jul 2020 13:06:05: 15000000 INFO @ Wed, 22 Jul 2020 13:06:06: 11000000 INFO @ Wed, 22 Jul 2020 13:06:10: 8000000 INFO @ Wed, 22 Jul 2020 13:06:13: 16000000 INFO @ Wed, 22 Jul 2020 13:06:15: 12000000 INFO @ Wed, 22 Jul 2020 13:06:18: 9000000 INFO @ Wed, 22 Jul 2020 13:06:22: 17000000 INFO @ Wed, 22 Jul 2020 13:06:23: 13000000 INFO @ Wed, 22 Jul 2020 13:06:27: 10000000 INFO @ Wed, 22 Jul 2020 13:06:30: 18000000 INFO @ Wed, 22 Jul 2020 13:06:32: 14000000 INFO @ Wed, 22 Jul 2020 13:06:35: 11000000 INFO @ Wed, 22 Jul 2020 13:06:38: 19000000 INFO @ Wed, 22 Jul 2020 13:06:40: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:06:44: 12000000 INFO @ Wed, 22 Jul 2020 13:06:47: 20000000 INFO @ Wed, 22 Jul 2020 13:06:49: 16000000 INFO @ Wed, 22 Jul 2020 13:06:52: 13000000 INFO @ Wed, 22 Jul 2020 13:06:55: 21000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:06:58: 17000000 INFO @ Wed, 22 Jul 2020 13:07:01: 14000000 INFO @ Wed, 22 Jul 2020 13:07:04: 22000000 INFO @ Wed, 22 Jul 2020 13:07:06: 18000000 INFO @ Wed, 22 Jul 2020 13:07:09: 15000000 INFO @ Wed, 22 Jul 2020 13:07:12: 23000000 INFO @ Wed, 22 Jul 2020 13:07:15: 19000000 INFO @ Wed, 22 Jul 2020 13:07:17: 16000000 INFO @ Wed, 22 Jul 2020 13:07:21: 24000000 INFO @ Wed, 22 Jul 2020 13:07:24: 20000000 INFO @ Wed, 22 Jul 2020 13:07:26: 17000000 INFO @ Wed, 22 Jul 2020 13:07:29: 25000000 INFO @ Wed, 22 Jul 2020 13:07:32: 21000000 INFO @ Wed, 22 Jul 2020 13:07:33: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:07:33: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:07:33: #1 total tags in treatment: 10491403 INFO @ Wed, 22 Jul 2020 13:07:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:07:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:07:34: #1 tags after filtering in treatment: 5456414 INFO @ Wed, 22 Jul 2020 13:07:34: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Jul 2020 13:07:34: #1 finished! INFO @ Wed, 22 Jul 2020 13:07:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:07:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:07:34: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:07:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:07:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Wed, 22 Jul 2020 13:07:34: 18000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:07:41: 22000000 INFO @ Wed, 22 Jul 2020 13:07:42: 19000000 INFO @ Wed, 22 Jul 2020 13:07:49: 23000000 INFO @ Wed, 22 Jul 2020 13:07:51: 20000000 INFO @ Wed, 22 Jul 2020 13:07:58: 24000000 INFO @ Wed, 22 Jul 2020 13:07:59: 21000000 INFO @ Wed, 22 Jul 2020 13:08:06: 25000000 INFO @ Wed, 22 Jul 2020 13:08:08: 22000000 INFO @ Wed, 22 Jul 2020 13:08:10: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:08:10: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:08:10: #1 total tags in treatment: 10491403 INFO @ Wed, 22 Jul 2020 13:08:10: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:08:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:08:11: #1 tags after filtering in treatment: 5456414 INFO @ Wed, 22 Jul 2020 13:08:11: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Jul 2020 13:08:11: #1 finished! INFO @ Wed, 22 Jul 2020 13:08:11: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:08:11: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:08:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:08:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:08:16: 23000000 INFO @ Wed, 22 Jul 2020 13:08:23: 24000000 INFO @ Wed, 22 Jul 2020 13:08:31: 25000000 INFO @ Wed, 22 Jul 2020 13:08:35: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 13:08:35: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 13:08:35: #1 total tags in treatment: 10491403 INFO @ Wed, 22 Jul 2020 13:08:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:08:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:08:35: #1 tags after filtering in treatment: 5456414 INFO @ Wed, 22 Jul 2020 13:08:35: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Jul 2020 13:08:35: #1 finished! INFO @ Wed, 22 Jul 2020 13:08:35: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:08:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:08:36: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:08:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:08:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6911497/SRX6911497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling