Job ID = 5791217 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,370,947 reads read : 18,741,894 reads written : 9,370,947 reads 0-length : 9,370,947 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:04 9370947 reads; of these: 9370947 (100.00%) were unpaired; of these: 503483 (5.37%) aligned 0 times 7864106 (83.92%) aligned exactly 1 time 1003358 (10.71%) aligned >1 times 94.63% overall alignment rate Time searching: 00:01:04 Overall time: 00:01:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3439753 / 8867464 = 0.3879 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:06:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:06:04: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:06:04: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:06:11: 1000000 INFO @ Wed, 22 Apr 2020 09:06:18: 2000000 INFO @ Wed, 22 Apr 2020 09:06:24: 3000000 INFO @ Wed, 22 Apr 2020 09:06:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:06:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:06:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:06:34: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:06:37: 5000000 INFO @ Wed, 22 Apr 2020 09:06:40: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 09:06:40: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 09:06:40: #1 total tags in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:06:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:06:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:06:40: #1 tags after filtering in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:06:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:06:40: #1 finished! INFO @ Wed, 22 Apr 2020 09:06:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:06:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:06:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:06:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:06:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:06:42: 1000000 INFO @ Wed, 22 Apr 2020 09:06:48: 2000000 INFO @ Wed, 22 Apr 2020 09:06:55: 3000000 INFO @ Wed, 22 Apr 2020 09:07:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:07:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:07:04: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:07:04: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:07:08: 5000000 INFO @ Wed, 22 Apr 2020 09:07:11: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 09:07:11: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 09:07:11: #1 total tags in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:07:11: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:07:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:07:11: #1 tags after filtering in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:07:11: #1 finished! INFO @ Wed, 22 Apr 2020 09:07:11: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:07:11: 1000000 INFO @ Wed, 22 Apr 2020 09:07:11: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:07:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:07:18: 2000000 INFO @ Wed, 22 Apr 2020 09:07:24: 3000000 INFO @ Wed, 22 Apr 2020 09:07:30: 4000000 INFO @ Wed, 22 Apr 2020 09:07:37: 5000000 INFO @ Wed, 22 Apr 2020 09:07:39: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 09:07:39: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 09:07:39: #1 total tags in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:07:39: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:07:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:07:39: #1 tags after filtering in treatment: 5427711 INFO @ Wed, 22 Apr 2020 09:07:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:07:39: #1 finished! INFO @ Wed, 22 Apr 2020 09:07:39: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:07:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:07:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:07:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:07:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6879622/SRX6879622.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。