Job ID = 4289511 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,241,016 reads read : 27,241,016 reads written : 27,241,016 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:52 27241016 reads; of these: 27241016 (100.00%) were unpaired; of these: 2294587 (8.42%) aligned 0 times 22482456 (82.53%) aligned exactly 1 time 2463973 (9.05%) aligned >1 times 91.58% overall alignment rate Time searching: 00:04:52 Overall time: 00:04:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14674553 / 24946429 = 0.5882 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:52:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:52:34: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:52:34: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:52:41: 1000000 INFO @ Tue, 10 Dec 2019 14:52:48: 2000000 INFO @ Tue, 10 Dec 2019 14:52:55: 3000000 INFO @ Tue, 10 Dec 2019 14:53:01: 4000000 INFO @ Tue, 10 Dec 2019 14:53:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:53:04: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:53:04: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:53:08: 5000000 INFO @ Tue, 10 Dec 2019 14:53:11: 1000000 INFO @ Tue, 10 Dec 2019 14:53:15: 6000000 INFO @ Tue, 10 Dec 2019 14:53:18: 2000000 INFO @ Tue, 10 Dec 2019 14:53:22: 7000000 INFO @ Tue, 10 Dec 2019 14:53:25: 3000000 INFO @ Tue, 10 Dec 2019 14:53:29: 8000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:53:32: 4000000 INFO @ Tue, 10 Dec 2019 14:53:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:53:35: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:53:35: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:53:36: 9000000 INFO @ Tue, 10 Dec 2019 14:53:39: 5000000 INFO @ Tue, 10 Dec 2019 14:53:43: 1000000 INFO @ Tue, 10 Dec 2019 14:53:44: 10000000 INFO @ Tue, 10 Dec 2019 14:53:46: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:53:46: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:53:46: #1 total tags in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:53:46: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:53:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:53:46: #1 tags after filtering in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:53:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:53:46: #1 finished! INFO @ Tue, 10 Dec 2019 14:53:46: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:53:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:53:47: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:53:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:53:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:53:47: 6000000 INFO @ Tue, 10 Dec 2019 14:53:50: 2000000 INFO @ Tue, 10 Dec 2019 14:53:55: 7000000 INFO @ Tue, 10 Dec 2019 14:53:58: 3000000 INFO @ Tue, 10 Dec 2019 14:54:03: 8000000 INFO @ Tue, 10 Dec 2019 14:54:05: 4000000 INFO @ Tue, 10 Dec 2019 14:54:10: 9000000 INFO @ Tue, 10 Dec 2019 14:54:13: 5000000 INFO @ Tue, 10 Dec 2019 14:54:18: 10000000 INFO @ Tue, 10 Dec 2019 14:54:20: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:54:20: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:54:20: #1 total tags in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:54:20: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:54:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:54:20: #1 tags after filtering in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:54:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:54:20: #1 finished! INFO @ Tue, 10 Dec 2019 14:54:20: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:54:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:54:21: 6000000 INFO @ Tue, 10 Dec 2019 14:54:21: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:54:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:54:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:54:27: 7000000 INFO @ Tue, 10 Dec 2019 14:54:34: 8000000 INFO @ Tue, 10 Dec 2019 14:54:41: 9000000 INFO @ Tue, 10 Dec 2019 14:54:48: 10000000 INFO @ Tue, 10 Dec 2019 14:54:50: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:54:50: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:54:50: #1 total tags in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:54:50: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:54:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:54:50: #1 tags after filtering in treatment: 10271876 INFO @ Tue, 10 Dec 2019 14:54:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:54:50: #1 finished! INFO @ Tue, 10 Dec 2019 14:54:50: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:54:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:54:51: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:54:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:54:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678276/SRX6678276.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。