Job ID = 4289465 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,944,434 reads read : 27,944,434 reads written : 27,944,434 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:08 27944434 reads; of these: 27944434 (100.00%) were unpaired; of these: 2181920 (7.81%) aligned 0 times 23232776 (83.14%) aligned exactly 1 time 2529738 (9.05%) aligned >1 times 92.19% overall alignment rate Time searching: 00:05:08 Overall time: 00:05:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15119243 / 25762514 = 0.5869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:48:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:48:36: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:48:36: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:48:43: 1000000 INFO @ Tue, 10 Dec 2019 14:48:51: 2000000 INFO @ Tue, 10 Dec 2019 14:48:58: 3000000 INFO @ Tue, 10 Dec 2019 14:49:05: 4000000 INFO @ Tue, 10 Dec 2019 14:49:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:49:06: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:49:06: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:49:12: 5000000 INFO @ Tue, 10 Dec 2019 14:49:14: 1000000 INFO @ Tue, 10 Dec 2019 14:49:20: 6000000 INFO @ Tue, 10 Dec 2019 14:49:23: 2000000 INFO @ Tue, 10 Dec 2019 14:49:27: 7000000 INFO @ Tue, 10 Dec 2019 14:49:31: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:49:34: 8000000 INFO @ Tue, 10 Dec 2019 14:49:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:49:36: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:49:36: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:49:40: 4000000 INFO @ Tue, 10 Dec 2019 14:49:41: 9000000 INFO @ Tue, 10 Dec 2019 14:49:45: 1000000 INFO @ Tue, 10 Dec 2019 14:49:48: 10000000 INFO @ Tue, 10 Dec 2019 14:49:49: 5000000 INFO @ Tue, 10 Dec 2019 14:49:53: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:49:53: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:49:53: #1 total tags in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:49:53: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:49:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:49:53: #1 tags after filtering in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:49:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:49:53: #1 finished! INFO @ Tue, 10 Dec 2019 14:49:53: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:49:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:49:54: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:49:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:49:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:49:54: 2000000 INFO @ Tue, 10 Dec 2019 14:49:57: 6000000 INFO @ Tue, 10 Dec 2019 14:50:03: 3000000 INFO @ Tue, 10 Dec 2019 14:50:05: 7000000 INFO @ Tue, 10 Dec 2019 14:50:11: 4000000 INFO @ Tue, 10 Dec 2019 14:50:14: 8000000 INFO @ Tue, 10 Dec 2019 14:50:19: 5000000 INFO @ Tue, 10 Dec 2019 14:50:22: 9000000 INFO @ Tue, 10 Dec 2019 14:50:27: 6000000 INFO @ Tue, 10 Dec 2019 14:50:30: 10000000 INFO @ Tue, 10 Dec 2019 14:50:35: 7000000 INFO @ Tue, 10 Dec 2019 14:50:36: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:50:36: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:50:36: #1 total tags in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:50:36: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:50:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:50:36: #1 tags after filtering in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:50:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:50:36: #1 finished! INFO @ Tue, 10 Dec 2019 14:50:36: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:50:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:50:37: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:50:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:50:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:50:43: 8000000 INFO @ Tue, 10 Dec 2019 14:50:52: 9000000 INFO @ Tue, 10 Dec 2019 14:51:00: 10000000 INFO @ Tue, 10 Dec 2019 14:51:05: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:51:05: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:51:05: #1 total tags in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:51:05: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:51:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:51:05: #1 tags after filtering in treatment: 10643271 INFO @ Tue, 10 Dec 2019 14:51:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:51:05: #1 finished! INFO @ Tue, 10 Dec 2019 14:51:05: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:51:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:51:06: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:51:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:51:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678267/SRX6678267.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。