Job ID = 4289353 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,808,349 reads read : 26,808,349 reads written : 26,808,349 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:18 26808349 reads; of these: 26808349 (100.00%) were unpaired; of these: 1182560 (4.41%) aligned 0 times 23108616 (86.20%) aligned exactly 1 time 2517173 (9.39%) aligned >1 times 95.59% overall alignment rate Time searching: 00:05:18 Overall time: 00:05:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13383681 / 25625789 = 0.5223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:36:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:36:31: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:36:31: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:36:39: 1000000 INFO @ Tue, 10 Dec 2019 14:36:47: 2000000 INFO @ Tue, 10 Dec 2019 14:36:55: 3000000 INFO @ Tue, 10 Dec 2019 14:37:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:37:01: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:37:01: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:37:03: 4000000 INFO @ Tue, 10 Dec 2019 14:37:09: 1000000 INFO @ Tue, 10 Dec 2019 14:37:12: 5000000 INFO @ Tue, 10 Dec 2019 14:37:17: 2000000 INFO @ Tue, 10 Dec 2019 14:37:20: 6000000 INFO @ Tue, 10 Dec 2019 14:37:25: 3000000 INFO @ Tue, 10 Dec 2019 14:37:28: 7000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:37:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:37:31: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:37:31: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:37:33: 4000000 INFO @ Tue, 10 Dec 2019 14:37:36: 8000000 INFO @ Tue, 10 Dec 2019 14:37:39: 1000000 INFO @ Tue, 10 Dec 2019 14:37:41: 5000000 INFO @ Tue, 10 Dec 2019 14:37:44: 9000000 INFO @ Tue, 10 Dec 2019 14:37:46: 2000000 INFO @ Tue, 10 Dec 2019 14:37:50: 6000000 INFO @ Tue, 10 Dec 2019 14:37:52: 10000000 INFO @ Tue, 10 Dec 2019 14:37:53: 3000000 INFO @ Tue, 10 Dec 2019 14:37:58: 7000000 INFO @ Tue, 10 Dec 2019 14:38:00: 11000000 INFO @ Tue, 10 Dec 2019 14:38:01: 4000000 INFO @ Tue, 10 Dec 2019 14:38:06: 8000000 INFO @ Tue, 10 Dec 2019 14:38:08: 5000000 INFO @ Tue, 10 Dec 2019 14:38:08: 12000000 INFO @ Tue, 10 Dec 2019 14:38:10: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:38:10: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:38:10: #1 total tags in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:10: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:38:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:38:10: #1 tags after filtering in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:38:10: #1 finished! INFO @ Tue, 10 Dec 2019 14:38:10: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:38:11: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:38:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:38:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:38:13: 9000000 INFO @ Tue, 10 Dec 2019 14:38:15: 6000000 INFO @ Tue, 10 Dec 2019 14:38:21: 10000000 INFO @ Tue, 10 Dec 2019 14:38:22: 7000000 INFO @ Tue, 10 Dec 2019 14:38:29: 11000000 INFO @ Tue, 10 Dec 2019 14:38:29: 8000000 INFO @ Tue, 10 Dec 2019 14:38:36: 9000000 INFO @ Tue, 10 Dec 2019 14:38:37: 12000000 INFO @ Tue, 10 Dec 2019 14:38:39: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:38:39: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:38:39: #1 total tags in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:39: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:38:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:38:39: #1 tags after filtering in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:38:39: #1 finished! INFO @ Tue, 10 Dec 2019 14:38:39: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:38:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:38:40: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:38:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:38:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:38:43: 10000000 INFO @ Tue, 10 Dec 2019 14:38:50: 11000000 INFO @ Tue, 10 Dec 2019 14:38:57: 12000000 INFO @ Tue, 10 Dec 2019 14:38:59: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:38:59: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:38:59: #1 total tags in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:59: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:38:59: #1 tags after filtering in treatment: 12242108 INFO @ Tue, 10 Dec 2019 14:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:38:59: #1 finished! INFO @ Tue, 10 Dec 2019 14:38:59: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:39:00: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:39:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:39:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678249/SRX6678249.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。