Job ID = 4289331 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,497,934 reads read : 22,497,934 reads written : 22,497,934 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:27 22497934 reads; of these: 22497934 (100.00%) were unpaired; of these: 1222968 (5.44%) aligned 0 times 19175330 (85.23%) aligned exactly 1 time 2099636 (9.33%) aligned >1 times 94.56% overall alignment rate Time searching: 00:04:27 Overall time: 00:04:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14522825 / 21274966 = 0.6826 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:22:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:22:55: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:22:55: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:23:03: 1000000 INFO @ Tue, 10 Dec 2019 14:23:10: 2000000 INFO @ Tue, 10 Dec 2019 14:23:18: 3000000 INFO @ Tue, 10 Dec 2019 14:23:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:23:24: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:23:24: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:23:25: 4000000 INFO @ Tue, 10 Dec 2019 14:23:33: 5000000 INFO @ Tue, 10 Dec 2019 14:23:34: 1000000 INFO @ Tue, 10 Dec 2019 14:23:40: 6000000 INFO @ Tue, 10 Dec 2019 14:23:43: 2000000 INFO @ Tue, 10 Dec 2019 14:23:46: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:23:46: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:23:46: #1 total tags in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:23:46: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:23:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:23:46: #1 tags after filtering in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:23:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:23:46: #1 finished! INFO @ Tue, 10 Dec 2019 14:23:46: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:23:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:23:47: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:23:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:23:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:23:51: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:23:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:23:55: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:23:55: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:24:00: 4000000 INFO @ Tue, 10 Dec 2019 14:24:03: 1000000 INFO @ Tue, 10 Dec 2019 14:24:09: 5000000 INFO @ Tue, 10 Dec 2019 14:24:11: 2000000 INFO @ Tue, 10 Dec 2019 14:24:17: 6000000 INFO @ Tue, 10 Dec 2019 14:24:19: 3000000 INFO @ Tue, 10 Dec 2019 14:24:24: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:24:24: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:24:24: #1 total tags in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:24:24: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:24:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:24:24: #1 tags after filtering in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:24:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:24:24: #1 finished! INFO @ Tue, 10 Dec 2019 14:24:24: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:24:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:24:24: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:24:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:24:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:24:26: 4000000 INFO @ Tue, 10 Dec 2019 14:24:34: 5000000 INFO @ Tue, 10 Dec 2019 14:24:42: 6000000 INFO @ Tue, 10 Dec 2019 14:24:48: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:24:48: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:24:48: #1 total tags in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:24:48: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:24:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:24:48: #1 tags after filtering in treatment: 6752141 INFO @ Tue, 10 Dec 2019 14:24:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:24:48: #1 finished! INFO @ Tue, 10 Dec 2019 14:24:48: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:24:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:24:48: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:24:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:24:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678241/SRX6678241.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。