Job ID = 4289312 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-12-10T05:05:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-12-10T05:05:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-12-10T05:08:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-12-10T05:08:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-12-10T05:10:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-12-10T05:10:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 23,940,101 reads read : 23,940,101 reads written : 23,940,101 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 23940101 reads; of these: 23940101 (100.00%) were unpaired; of these: 965204 (4.03%) aligned 0 times 20384685 (85.15%) aligned exactly 1 time 2590212 (10.82%) aligned >1 times 95.97% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12966918 / 22974897 = 0.5644 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:21:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:21:34: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:21:34: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:21:44: 1000000 INFO @ Tue, 10 Dec 2019 14:21:53: 2000000 INFO @ Tue, 10 Dec 2019 14:22:02: 3000000 INFO @ Tue, 10 Dec 2019 14:22:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:22:04: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:22:04: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:22:11: 4000000 INFO @ Tue, 10 Dec 2019 14:22:14: 1000000 INFO @ Tue, 10 Dec 2019 14:22:19: 5000000 INFO @ Tue, 10 Dec 2019 14:22:24: 2000000 INFO @ Tue, 10 Dec 2019 14:22:28: 6000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:22:34: 3000000 INFO @ Tue, 10 Dec 2019 14:22:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:22:34: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:22:34: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:22:36: 7000000 INFO @ Tue, 10 Dec 2019 14:22:43: 1000000 INFO @ Tue, 10 Dec 2019 14:22:43: 4000000 INFO @ Tue, 10 Dec 2019 14:22:45: 8000000 INFO @ Tue, 10 Dec 2019 14:22:51: 2000000 INFO @ Tue, 10 Dec 2019 14:22:53: 5000000 INFO @ Tue, 10 Dec 2019 14:22:53: 9000000 INFO @ Tue, 10 Dec 2019 14:23:00: 3000000 INFO @ Tue, 10 Dec 2019 14:23:02: 10000000 INFO @ Tue, 10 Dec 2019 14:23:02: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:23:02: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:23:02: #1 total tags in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:02: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:23:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:23:02: #1 tags after filtering in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:23:02: #1 finished! INFO @ Tue, 10 Dec 2019 14:23:02: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:23:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:23:03: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:23:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:23:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:23:03: 6000000 INFO @ Tue, 10 Dec 2019 14:23:08: 4000000 INFO @ Tue, 10 Dec 2019 14:23:13: 7000000 INFO @ Tue, 10 Dec 2019 14:23:16: 5000000 INFO @ Tue, 10 Dec 2019 14:23:22: 8000000 INFO @ Tue, 10 Dec 2019 14:23:24: 6000000 INFO @ Tue, 10 Dec 2019 14:23:32: 9000000 INFO @ Tue, 10 Dec 2019 14:23:33: 7000000 INFO @ Tue, 10 Dec 2019 14:23:41: 10000000 INFO @ Tue, 10 Dec 2019 14:23:41: 8000000 INFO @ Tue, 10 Dec 2019 14:23:41: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:23:41: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:23:41: #1 total tags in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:41: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:23:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:23:41: #1 tags after filtering in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:23:41: #1 finished! INFO @ Tue, 10 Dec 2019 14:23:41: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:23:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:23:42: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:23:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:23:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:23:49: 9000000 INFO @ Tue, 10 Dec 2019 14:23:56: 10000000 INFO @ Tue, 10 Dec 2019 14:23:56: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:23:56: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:23:56: #1 total tags in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:56: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:23:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:23:56: #1 tags after filtering in treatment: 10007979 INFO @ Tue, 10 Dec 2019 14:23:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:23:56: #1 finished! INFO @ Tue, 10 Dec 2019 14:23:56: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:23:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:23:57: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:23:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:23:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6678234/SRX6678234.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。