Job ID = 4289295 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,503,058 reads read : 69,006,116 reads written : 34,503,058 reads 0-length : 34,503,058 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:37 34503058 reads; of these: 34503058 (100.00%) were unpaired; of these: 509431 (1.48%) aligned 0 times 30471742 (88.32%) aligned exactly 1 time 3521885 (10.21%) aligned >1 times 98.52% overall alignment rate Time searching: 00:06:37 Overall time: 00:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18278153 / 33993627 = 0.5377 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:27:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:27:38: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:27:38: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:27:47: 1000000 INFO @ Tue, 10 Dec 2019 14:27:56: 2000000 INFO @ Tue, 10 Dec 2019 14:28:05: 3000000 INFO @ Tue, 10 Dec 2019 14:28:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:28:08: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:28:08: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:28:13: 4000000 INFO @ Tue, 10 Dec 2019 14:28:18: 1000000 INFO @ Tue, 10 Dec 2019 14:28:22: 5000000 INFO @ Tue, 10 Dec 2019 14:28:28: 2000000 INFO @ Tue, 10 Dec 2019 14:28:31: 6000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:28:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:28:38: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:28:38: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:28:39: 3000000 INFO @ Tue, 10 Dec 2019 14:28:39: 7000000 INFO @ Tue, 10 Dec 2019 14:28:47: 1000000 INFO @ Tue, 10 Dec 2019 14:28:48: 8000000 INFO @ Tue, 10 Dec 2019 14:28:49: 4000000 INFO @ Tue, 10 Dec 2019 14:28:55: 2000000 INFO @ Tue, 10 Dec 2019 14:28:57: 9000000 INFO @ Tue, 10 Dec 2019 14:28:59: 5000000 INFO @ Tue, 10 Dec 2019 14:29:04: 3000000 INFO @ Tue, 10 Dec 2019 14:29:05: 10000000 INFO @ Tue, 10 Dec 2019 14:29:09: 6000000 INFO @ Tue, 10 Dec 2019 14:29:13: 4000000 INFO @ Tue, 10 Dec 2019 14:29:14: 11000000 INFO @ Tue, 10 Dec 2019 14:29:20: 7000000 INFO @ Tue, 10 Dec 2019 14:29:21: 5000000 INFO @ Tue, 10 Dec 2019 14:29:23: 12000000 INFO @ Tue, 10 Dec 2019 14:29:30: 8000000 INFO @ Tue, 10 Dec 2019 14:29:30: 6000000 INFO @ Tue, 10 Dec 2019 14:29:31: 13000000 INFO @ Tue, 10 Dec 2019 14:29:39: 7000000 INFO @ Tue, 10 Dec 2019 14:29:40: 9000000 INFO @ Tue, 10 Dec 2019 14:29:40: 14000000 INFO @ Tue, 10 Dec 2019 14:29:48: 8000000 INFO @ Tue, 10 Dec 2019 14:29:49: 15000000 INFO @ Tue, 10 Dec 2019 14:29:51: 10000000 INFO @ Tue, 10 Dec 2019 14:29:56: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:29:56: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:29:56: #1 total tags in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:29:56: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:29:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:29:56: #1 tags after filtering in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:29:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:29:56: #1 finished! INFO @ Tue, 10 Dec 2019 14:29:56: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:29:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:29:57: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:29:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:29:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:29:57: 9000000 INFO @ Tue, 10 Dec 2019 14:30:01: 11000000 INFO @ Tue, 10 Dec 2019 14:30:06: 10000000 INFO @ Tue, 10 Dec 2019 14:30:11: 12000000 INFO @ Tue, 10 Dec 2019 14:30:15: 11000000 INFO @ Tue, 10 Dec 2019 14:30:21: 13000000 INFO @ Tue, 10 Dec 2019 14:30:23: 12000000 INFO @ Tue, 10 Dec 2019 14:30:31: 14000000 INFO @ Tue, 10 Dec 2019 14:30:32: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 10 Dec 2019 14:30:41: 14000000 INFO @ Tue, 10 Dec 2019 14:30:42: 15000000 BigWig に変換しました。 INFO @ Tue, 10 Dec 2019 14:30:49: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:30:49: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:30:49: #1 total tags in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:30:49: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:30:49: #1 tags after filtering in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:30:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:30:49: #1 finished! INFO @ Tue, 10 Dec 2019 14:30:49: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:30:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:30:49: 15000000 INFO @ Tue, 10 Dec 2019 14:30:50: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:30:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:30:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:30:55: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:30:55: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:30:55: #1 total tags in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:30:55: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:30:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:30:56: #1 tags after filtering in treatment: 15715474 INFO @ Tue, 10 Dec 2019 14:30:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:30:56: #1 finished! INFO @ Tue, 10 Dec 2019 14:30:56: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:30:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:30:57: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:30:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:30:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635443/SRX6635443.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling