Job ID = 4289271 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,593,100 reads read : 69,186,200 reads written : 34,593,100 reads 0-length : 34,593,100 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:24 34593100 reads; of these: 34593100 (100.00%) were unpaired; of these: 805403 (2.33%) aligned 0 times 26164255 (75.63%) aligned exactly 1 time 7623442 (22.04%) aligned >1 times 97.67% overall alignment rate Time searching: 00:06:24 Overall time: 00:06:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18922900 / 33787697 = 0.5601 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:17:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:17:28: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:17:28: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:17:35: 1000000 INFO @ Tue, 10 Dec 2019 14:17:41: 2000000 INFO @ Tue, 10 Dec 2019 14:17:48: 3000000 INFO @ Tue, 10 Dec 2019 14:17:55: 4000000 INFO @ Tue, 10 Dec 2019 14:17:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:17:58: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:17:58: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:18:02: 5000000 INFO @ Tue, 10 Dec 2019 14:18:04: 1000000 INFO @ Tue, 10 Dec 2019 14:18:09: 6000000 INFO @ Tue, 10 Dec 2019 14:18:10: 2000000 INFO @ Tue, 10 Dec 2019 14:18:16: 7000000 INFO @ Tue, 10 Dec 2019 14:18:17: 3000000 INFO @ Tue, 10 Dec 2019 14:18:22: 8000000 INFO @ Tue, 10 Dec 2019 14:18:23: 4000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:18:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:18:28: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:18:28: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:18:29: 5000000 INFO @ Tue, 10 Dec 2019 14:18:30: 9000000 INFO @ Tue, 10 Dec 2019 14:18:35: 1000000 INFO @ Tue, 10 Dec 2019 14:18:35: 6000000 INFO @ Tue, 10 Dec 2019 14:18:37: 10000000 INFO @ Tue, 10 Dec 2019 14:18:42: 7000000 INFO @ Tue, 10 Dec 2019 14:18:43: 2000000 INFO @ Tue, 10 Dec 2019 14:18:45: 11000000 INFO @ Tue, 10 Dec 2019 14:18:48: 8000000 INFO @ Tue, 10 Dec 2019 14:18:51: 3000000 INFO @ Tue, 10 Dec 2019 14:18:53: 12000000 INFO @ Tue, 10 Dec 2019 14:18:55: 9000000 INFO @ Tue, 10 Dec 2019 14:18:59: 4000000 INFO @ Tue, 10 Dec 2019 14:19:01: 13000000 INFO @ Tue, 10 Dec 2019 14:19:01: 10000000 INFO @ Tue, 10 Dec 2019 14:19:06: 5000000 INFO @ Tue, 10 Dec 2019 14:19:07: 11000000 INFO @ Tue, 10 Dec 2019 14:19:08: 14000000 INFO @ Tue, 10 Dec 2019 14:19:14: 12000000 INFO @ Tue, 10 Dec 2019 14:19:14: 6000000 INFO @ Tue, 10 Dec 2019 14:19:14: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:19:14: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:19:14: #1 total tags in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:19:14: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:19:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:19:14: #1 tags after filtering in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:19:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:19:14: #1 finished! INFO @ Tue, 10 Dec 2019 14:19:14: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:19:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:19:16: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:19:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:19:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:19:20: 13000000 INFO @ Tue, 10 Dec 2019 14:19:21: 7000000 INFO @ Tue, 10 Dec 2019 14:19:26: 14000000 INFO @ Tue, 10 Dec 2019 14:19:28: 8000000 INFO @ Tue, 10 Dec 2019 14:19:32: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:19:32: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:19:32: #1 total tags in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:19:32: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:19:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:19:32: #1 tags after filtering in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:19:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:19:32: #1 finished! INFO @ Tue, 10 Dec 2019 14:19:32: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:19:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:19:33: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:19:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:19:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:19:34: 9000000 INFO @ Tue, 10 Dec 2019 14:19:41: 10000000 INFO @ Tue, 10 Dec 2019 14:19:48: 11000000 INFO @ Tue, 10 Dec 2019 14:19:55: 12000000 INFO @ Tue, 10 Dec 2019 14:20:02: 13000000 INFO @ Tue, 10 Dec 2019 14:20:09: 14000000 INFO @ Tue, 10 Dec 2019 14:20:15: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 14:20:15: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 14:20:15: #1 total tags in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:20:15: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:20:15: #1 tags after filtering in treatment: 14864797 INFO @ Tue, 10 Dec 2019 14:20:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:20:15: #1 finished! INFO @ Tue, 10 Dec 2019 14:20:15: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:20:16: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:20:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:20:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635432/SRX6635432.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。