Job ID = 4289206 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,324,445 reads read : 28,648,890 reads written : 14,324,445 reads 0-length : 14,324,445 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 14324445 reads; of these: 14324445 (100.00%) were unpaired; of these: 582156 (4.06%) aligned 0 times 11053235 (77.16%) aligned exactly 1 time 2689054 (18.77%) aligned >1 times 95.94% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6102440 / 13742289 = 0.4441 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:57:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:57:46: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:57:46: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:57:54: 1000000 INFO @ Tue, 10 Dec 2019 13:58:03: 2000000 INFO @ Tue, 10 Dec 2019 13:58:12: 3000000 INFO @ Tue, 10 Dec 2019 13:58:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:16: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:16: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:22: 4000000 INFO @ Tue, 10 Dec 2019 13:58:26: 1000000 INFO @ Tue, 10 Dec 2019 13:58:31: 5000000 INFO @ Tue, 10 Dec 2019 13:58:37: 2000000 INFO @ Tue, 10 Dec 2019 13:58:42: 6000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:58:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:46: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:46: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:48: 3000000 INFO @ Tue, 10 Dec 2019 13:58:51: 7000000 INFO @ Tue, 10 Dec 2019 13:58:54: 1000000 INFO @ Tue, 10 Dec 2019 13:58:58: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:58:58: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:58:58: #1 total tags in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:58:58: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:58:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:58:58: #1 tags after filtering in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:58:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:58:58: #1 finished! INFO @ Tue, 10 Dec 2019 13:58:58: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:58:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:58:58: 4000000 INFO @ Tue, 10 Dec 2019 13:58:59: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:58:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:58:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:59:03: 2000000 INFO @ Tue, 10 Dec 2019 13:59:08: 5000000 INFO @ Tue, 10 Dec 2019 13:59:11: 3000000 INFO @ Tue, 10 Dec 2019 13:59:18: 6000000 INFO @ Tue, 10 Dec 2019 13:59:19: 4000000 INFO @ Tue, 10 Dec 2019 13:59:27: 5000000 INFO @ Tue, 10 Dec 2019 13:59:27: 7000000 INFO @ Tue, 10 Dec 2019 13:59:33: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:59:33: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:59:33: #1 total tags in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:59:33: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:59:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:59:34: #1 tags after filtering in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:59:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:59:34: #1 finished! INFO @ Tue, 10 Dec 2019 13:59:34: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:59:34: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:59:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:59:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:59:35: 6000000 INFO @ Tue, 10 Dec 2019 13:59:43: 7000000 INFO @ Tue, 10 Dec 2019 13:59:48: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:59:48: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:59:48: #1 total tags in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:59:48: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:59:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:59:48: #1 tags after filtering in treatment: 7639849 INFO @ Tue, 10 Dec 2019 13:59:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:59:48: #1 finished! INFO @ Tue, 10 Dec 2019 13:59:48: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:59:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:59:49: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:59:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:59:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635418/SRX6635418.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。