Job ID = 4289174 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,059,506 reads read : 32,119,012 reads written : 16,059,506 reads 0-length : 16,059,506 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 16059506 reads; of these: 16059506 (100.00%) were unpaired; of these: 1132698 (7.05%) aligned 0 times 11984896 (74.63%) aligned exactly 1 time 2941912 (18.32%) aligned >1 times 92.95% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7230477 / 14926808 = 0.4844 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:55:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:55:00: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:55:00: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:55:08: 1000000 INFO @ Tue, 10 Dec 2019 13:55:17: 2000000 INFO @ Tue, 10 Dec 2019 13:55:25: 3000000 INFO @ Tue, 10 Dec 2019 13:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:55:30: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:55:30: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:55:33: 4000000 INFO @ Tue, 10 Dec 2019 13:55:39: 1000000 INFO @ Tue, 10 Dec 2019 13:55:41: 5000000 INFO @ Tue, 10 Dec 2019 13:55:48: 2000000 INFO @ Tue, 10 Dec 2019 13:55:49: 6000000 INFO @ Tue, 10 Dec 2019 13:55:57: 7000000 INFO @ Tue, 10 Dec 2019 13:55:57: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:56:00: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:56:00: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:56:03: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:56:03: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:56:03: #1 total tags in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:56:03: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:56:03: #1 tags after filtering in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:56:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:56:03: #1 finished! INFO @ Tue, 10 Dec 2019 13:56:03: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:56:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:56:03: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:56:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:56:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:56:07: 4000000 INFO @ Tue, 10 Dec 2019 13:56:11: 1000000 INFO @ Tue, 10 Dec 2019 13:56:16: 5000000 INFO @ Tue, 10 Dec 2019 13:56:21: 2000000 INFO @ Tue, 10 Dec 2019 13:56:25: 6000000 INFO @ Tue, 10 Dec 2019 13:56:31: 3000000 INFO @ Tue, 10 Dec 2019 13:56:34: 7000000 INFO @ Tue, 10 Dec 2019 13:56:40: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:56:40: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:56:40: #1 total tags in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:56:40: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:56:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:56:40: #1 tags after filtering in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:56:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:56:40: #1 finished! INFO @ Tue, 10 Dec 2019 13:56:40: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:56:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:56:41: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:56:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:56:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:56:41: 4000000 INFO @ Tue, 10 Dec 2019 13:56:51: 5000000 INFO @ Tue, 10 Dec 2019 13:57:01: 6000000 INFO @ Tue, 10 Dec 2019 13:57:11: 7000000 INFO @ Tue, 10 Dec 2019 13:57:17: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:57:17: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:57:17: #1 total tags in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:57:17: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:57:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:57:17: #1 tags after filtering in treatment: 7696331 INFO @ Tue, 10 Dec 2019 13:57:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:57:17: #1 finished! INFO @ Tue, 10 Dec 2019 13:57:17: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:57:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:57:18: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:57:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:57:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635414/SRX6635414.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。