Job ID = 4289172 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,901,353 reads read : 39,802,706 reads written : 19,901,353 reads 0-length : 19,901,353 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:59 19901353 reads; of these: 19901353 (100.00%) were unpaired; of these: 3064003 (15.40%) aligned 0 times 14239835 (71.55%) aligned exactly 1 time 2597515 (13.05%) aligned >1 times 84.60% overall alignment rate Time searching: 00:09:00 Overall time: 00:09:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8092899 / 16837350 = 0.4807 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:11:26: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:11:26: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:11:43: 1000000 INFO @ Tue, 10 Dec 2019 14:11:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:11:56: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:11:56: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:12:00: 2000000 INFO @ Tue, 10 Dec 2019 14:12:11: 1000000 INFO @ Tue, 10 Dec 2019 14:12:16: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:12:24: 2000000 INFO @ Tue, 10 Dec 2019 14:12:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:12:26: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:12:26: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:12:33: 4000000 INFO @ Tue, 10 Dec 2019 14:12:38: 3000000 INFO @ Tue, 10 Dec 2019 14:12:40: 1000000 INFO @ Tue, 10 Dec 2019 14:12:49: 5000000 INFO @ Tue, 10 Dec 2019 14:12:51: 4000000 INFO @ Tue, 10 Dec 2019 14:12:55: 2000000 INFO @ Tue, 10 Dec 2019 14:13:03: 5000000 INFO @ Tue, 10 Dec 2019 14:13:06: 6000000 INFO @ Tue, 10 Dec 2019 14:13:09: 3000000 INFO @ Tue, 10 Dec 2019 14:13:16: 6000000 INFO @ Tue, 10 Dec 2019 14:13:22: 4000000 INFO @ Tue, 10 Dec 2019 14:13:22: 7000000 INFO @ Tue, 10 Dec 2019 14:13:29: 7000000 INFO @ Tue, 10 Dec 2019 14:13:35: 5000000 INFO @ Tue, 10 Dec 2019 14:13:40: 8000000 INFO @ Tue, 10 Dec 2019 14:13:42: 8000000 INFO @ Tue, 10 Dec 2019 14:13:49: 6000000 INFO @ Tue, 10 Dec 2019 14:13:52: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:13:52: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:13:52: #1 total tags in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:13:52: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:13:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:13:52: #1 tags after filtering in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:13:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:13:52: #1 finished! INFO @ Tue, 10 Dec 2019 14:13:52: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:13:52: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:13:52: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:13:52: #1 total tags in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:13:52: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:13:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:13:52: #1 tags after filtering in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:13:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:13:52: #1 finished! INFO @ Tue, 10 Dec 2019 14:13:52: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:13:52: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:13:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:13:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:13:53: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:13:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:13:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:14:01: 7000000 INFO @ Tue, 10 Dec 2019 14:14:14: 8000000 INFO @ Tue, 10 Dec 2019 14:14:23: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:14:23: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:14:23: #1 total tags in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:14:23: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:14:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:14:23: #1 tags after filtering in treatment: 8744451 INFO @ Tue, 10 Dec 2019 14:14:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:14:23: #1 finished! INFO @ Tue, 10 Dec 2019 14:14:23: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:14:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:14:24: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:14:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:14:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635413/SRX6635413.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。