Job ID = 4289170 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,137,462 reads read : 30,274,924 reads written : 15,137,462 reads 0-length : 15,137,462 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:43 15137462 reads; of these: 15137462 (100.00%) were unpaired; of these: 2285835 (15.10%) aligned 0 times 10862607 (71.76%) aligned exactly 1 time 1989020 (13.14%) aligned >1 times 84.90% overall alignment rate Time searching: 00:06:43 Overall time: 00:06:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6419810 / 12851627 = 0.4995 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:05:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:05:49: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:05:49: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:06:01: 1000000 INFO @ Tue, 10 Dec 2019 14:06:11: 2000000 INFO @ Tue, 10 Dec 2019 14:06:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:06:19: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:06:19: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:06:21: 3000000 INFO @ Tue, 10 Dec 2019 14:06:30: 1000000 INFO @ Tue, 10 Dec 2019 14:06:31: 4000000 INFO @ Tue, 10 Dec 2019 14:06:40: 2000000 INFO @ Tue, 10 Dec 2019 14:06:41: 5000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:06:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:06:49: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:06:49: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:06:50: 3000000 INFO @ Tue, 10 Dec 2019 14:06:52: 6000000 INFO @ Tue, 10 Dec 2019 14:06:57: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:06:57: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:06:57: #1 total tags in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:06:57: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:06:57: #1 tags after filtering in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:06:57: #1 finished! INFO @ Tue, 10 Dec 2019 14:06:57: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:06:58: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:06:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:06:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:07:01: 4000000 INFO @ Tue, 10 Dec 2019 14:07:01: 1000000 INFO @ Tue, 10 Dec 2019 14:07:11: 2000000 INFO @ Tue, 10 Dec 2019 14:07:11: 5000000 INFO @ Tue, 10 Dec 2019 14:07:21: 3000000 INFO @ Tue, 10 Dec 2019 14:07:21: 6000000 INFO @ Tue, 10 Dec 2019 14:07:25: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:07:25: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:07:25: #1 total tags in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:07:25: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:07:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:07:25: #1 tags after filtering in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:07:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:07:25: #1 finished! INFO @ Tue, 10 Dec 2019 14:07:25: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:07:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:07:26: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:07:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:07:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:07:30: 4000000 INFO @ Tue, 10 Dec 2019 14:07:40: 5000000 INFO @ Tue, 10 Dec 2019 14:07:49: 6000000 INFO @ Tue, 10 Dec 2019 14:07:53: #1 tag size is determined as 125 bps INFO @ Tue, 10 Dec 2019 14:07:53: #1 tag size = 125 INFO @ Tue, 10 Dec 2019 14:07:53: #1 total tags in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:07:53: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:07:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:07:53: #1 tags after filtering in treatment: 6431817 INFO @ Tue, 10 Dec 2019 14:07:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:07:53: #1 finished! INFO @ Tue, 10 Dec 2019 14:07:53: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:07:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:07:54: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 14:07:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:07:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6635412/SRX6635412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。