Job ID = 4289163 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,733,938 reads read : 11,467,876 reads written : 5,733,938 reads 0-length : 5,733,938 2019-12-10T04:46:35 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 11,754,708 reads read : 23,509,416 reads written : 11,754,708 reads 0-length : 11,754,708 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:03 17488646 reads; of these: 17488646 (100.00%) were unpaired; of these: 9108820 (52.08%) aligned 0 times 5117670 (29.26%) aligned exactly 1 time 3262156 (18.65%) aligned >1 times 47.92% overall alignment rate Time searching: 00:02:03 Overall time: 00:02:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4541366 / 8379826 = 0.5419 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:54:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:54:12: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:54:12: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:54:22: 1000000 INFO @ Tue, 10 Dec 2019 13:54:31: 2000000 INFO @ Tue, 10 Dec 2019 13:54:41: 3000000 INFO @ Tue, 10 Dec 2019 13:54:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:54:42: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:54:42: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:54:48: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:54:48: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:54:48: #1 total tags in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:54:48: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:54:48: #1 tags after filtering in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:54:48: #1 finished! INFO @ Tue, 10 Dec 2019 13:54:48: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:54:48: #2 number of paired peaks: 41 WARNING @ Tue, 10 Dec 2019 13:54:48: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:54:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:54:50: 1000000 INFO @ Tue, 10 Dec 2019 13:54:57: 2000000 INFO @ Tue, 10 Dec 2019 13:55:05: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:55:11: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:55:11: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:55:11: #1 total tags in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:55:11: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:55:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:55:11: #1 tags after filtering in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:55:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:55:11: #1 finished! INFO @ Tue, 10 Dec 2019 13:55:11: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:55:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:55:11: #2 number of paired peaks: 41 WARNING @ Tue, 10 Dec 2019 13:55:11: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:55:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:55:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:55:12: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:55:12: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:55:20: 1000000 INFO @ Tue, 10 Dec 2019 13:55:27: 2000000 INFO @ Tue, 10 Dec 2019 13:55:35: 3000000 INFO @ Tue, 10 Dec 2019 13:55:41: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:55:41: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:55:41: #1 total tags in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:55:41: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:55:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:55:41: #1 tags after filtering in treatment: 3838460 INFO @ Tue, 10 Dec 2019 13:55:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:55:41: #1 finished! INFO @ Tue, 10 Dec 2019 13:55:41: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:55:41: #2 number of paired peaks: 41 WARNING @ Tue, 10 Dec 2019 13:55:41: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:55:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616774/SRX6616774.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。