Job ID = 4289156 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,921,675 reads read : 31,843,350 reads written : 15,921,675 reads 0-length : 15,921,675 spots read : 15,626,861 reads read : 31,253,722 reads written : 15,626,861 reads 0-length : 15,626,861 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:29 31548536 reads; of these: 31548536 (100.00%) were unpaired; of these: 20368825 (64.56%) aligned 0 times 6676219 (21.16%) aligned exactly 1 time 4503492 (14.27%) aligned >1 times 35.44% overall alignment rate Time searching: 00:04:29 Overall time: 00:04:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5562006 / 11179711 = 0.4975 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:57:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:57:35: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:57:35: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:57:44: 1000000 INFO @ Tue, 10 Dec 2019 13:57:53: 2000000 INFO @ Tue, 10 Dec 2019 13:58:01: 3000000 INFO @ Tue, 10 Dec 2019 13:58:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:06: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:06: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:10: 4000000 INFO @ Tue, 10 Dec 2019 13:58:14: 1000000 INFO @ Tue, 10 Dec 2019 13:58:19: 5000000 INFO @ Tue, 10 Dec 2019 13:58:24: 2000000 INFO @ Tue, 10 Dec 2019 13:58:24: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:58:24: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:58:24: #1 total tags in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:58:24: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:58:24: #1 tags after filtering in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:58:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:58:24: #1 finished! INFO @ Tue, 10 Dec 2019 13:58:24: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:58:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:58:25: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:58:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:58:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:58:33: 3000000 INFO @ Tue, 10 Dec 2019 13:58:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:58:35: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:58:35: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:58:40: 4000000 INFO @ Tue, 10 Dec 2019 13:58:45: 1000000 INFO @ Tue, 10 Dec 2019 13:58:48: 5000000 INFO @ Tue, 10 Dec 2019 13:58:52: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:58:52: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:58:52: #1 total tags in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:58:52: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:58:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:58:52: #1 tags after filtering in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:58:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:58:52: #1 finished! INFO @ Tue, 10 Dec 2019 13:58:52: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:58:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:58:53: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:58:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:58:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:58:53: 2000000 INFO @ Tue, 10 Dec 2019 13:59:02: 3000000 INFO @ Tue, 10 Dec 2019 13:59:11: 4000000 INFO @ Tue, 10 Dec 2019 13:59:20: 5000000 INFO @ Tue, 10 Dec 2019 13:59:26: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 13:59:26: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 13:59:26: #1 total tags in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:59:26: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:59:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:59:26: #1 tags after filtering in treatment: 5617705 INFO @ Tue, 10 Dec 2019 13:59:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:59:26: #1 finished! INFO @ Tue, 10 Dec 2019 13:59:26: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:59:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:59:26: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:59:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:59:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616770/SRX6616770.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。