Job ID = 4289155 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,037,757 reads read : 22,075,514 reads written : 11,037,757 reads 0-length : 11,037,757 spots read : 10,827,638 reads read : 21,655,276 reads written : 10,827,638 reads 0-length : 10,827,638 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:28 21865395 reads; of these: 21865395 (100.00%) were unpaired; of these: 14327976 (65.53%) aligned 0 times 4511679 (20.63%) aligned exactly 1 time 3025740 (13.84%) aligned >1 times 34.47% overall alignment rate Time searching: 00:02:28 Overall time: 00:02:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3397872 / 7537419 = 0.4508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:29:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:29:49: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:29:49: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:29:59: 1000000 INFO @ Tue, 10 Dec 2019 14:30:09: 2000000 INFO @ Tue, 10 Dec 2019 14:30:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:30:19: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:30:19: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:30:19: 3000000 INFO @ Tue, 10 Dec 2019 14:30:30: 1000000 INFO @ Tue, 10 Dec 2019 14:30:31: 4000000 INFO @ Tue, 10 Dec 2019 14:30:32: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:30:32: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:30:32: #1 total tags in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:30:32: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:30:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:30:32: #1 tags after filtering in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:30:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:30:32: #1 finished! INFO @ Tue, 10 Dec 2019 14:30:32: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:30:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:30:32: #2 number of paired peaks: 35 WARNING @ Tue, 10 Dec 2019 14:30:32: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:30:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:30:40: 2000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:30:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:30:49: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:30:49: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:30:51: 3000000 INFO @ Tue, 10 Dec 2019 14:30:58: 1000000 INFO @ Tue, 10 Dec 2019 14:31:02: 4000000 INFO @ Tue, 10 Dec 2019 14:31:03: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:31:03: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:31:03: #1 total tags in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:31:03: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:31:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:31:03: #1 tags after filtering in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:31:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:31:03: #1 finished! INFO @ Tue, 10 Dec 2019 14:31:03: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:31:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:31:04: #2 number of paired peaks: 35 WARNING @ Tue, 10 Dec 2019 14:31:04: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:31:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:31:07: 2000000 INFO @ Tue, 10 Dec 2019 14:31:15: 3000000 INFO @ Tue, 10 Dec 2019 14:31:24: 4000000 INFO @ Tue, 10 Dec 2019 14:31:24: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 14:31:24: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 14:31:24: #1 total tags in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:31:24: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:31:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:31:25: #1 tags after filtering in treatment: 4139547 INFO @ Tue, 10 Dec 2019 14:31:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:31:25: #1 finished! INFO @ Tue, 10 Dec 2019 14:31:25: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:31:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:31:25: #2 number of paired peaks: 35 WARNING @ Tue, 10 Dec 2019 14:31:25: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:31:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6616769/SRX6616769.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。