Job ID = 5791204 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-22T00:02:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 9,095,974 reads read : 18,191,948 reads written : 18,191,948 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:34 9095974 reads; of these: 9095974 (100.00%) were paired; of these: 2969312 (32.64%) aligned concordantly 0 times 2165662 (23.81%) aligned concordantly exactly 1 time 3961000 (43.55%) aligned concordantly >1 times ---- 2969312 pairs aligned concordantly 0 times; of these: 325352 (10.96%) aligned discordantly 1 time ---- 2643960 pairs aligned 0 times concordantly or discordantly; of these: 5287920 mates make up the pairs; of these: 3933951 (74.40%) aligned 0 times 296283 (5.60%) aligned exactly 1 time 1057686 (20.00%) aligned >1 times 78.38% overall alignment rate Time searching: 00:07:34 Overall time: 00:07:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4268231 / 6395370 = 0.6674 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:18:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:18:56: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:18:56: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:19:05: 1000000 INFO @ Wed, 22 Apr 2020 09:19:13: 2000000 INFO @ Wed, 22 Apr 2020 09:19:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:19:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:19:26: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:19:26: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:19:30: 4000000 INFO @ Wed, 22 Apr 2020 09:19:36: 1000000 INFO @ Wed, 22 Apr 2020 09:19:39: 5000000 INFO @ Wed, 22 Apr 2020 09:19:45: 2000000 INFO @ Wed, 22 Apr 2020 09:19:46: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:19:46: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:19:46: #1 total tags in treatment: 2007180 INFO @ Wed, 22 Apr 2020 09:19:46: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:19:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:19:46: #1 tags after filtering in treatment: 1235898 INFO @ Wed, 22 Apr 2020 09:19:46: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Apr 2020 09:19:46: #1 finished! INFO @ Wed, 22 Apr 2020 09:19:46: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:19:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:19:46: #2 number of paired peaks: 44 WARNING @ Wed, 22 Apr 2020 09:19:46: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:19:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:19:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:19:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:19:56: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:19:56: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:20:03: 4000000 INFO @ Wed, 22 Apr 2020 09:20:06: 1000000 INFO @ Wed, 22 Apr 2020 09:20:12: 5000000 INFO @ Wed, 22 Apr 2020 09:20:15: 2000000 INFO @ Wed, 22 Apr 2020 09:20:19: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:20:19: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:20:19: #1 total tags in treatment: 2007180 INFO @ Wed, 22 Apr 2020 09:20:19: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:20:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:20:19: #1 tags after filtering in treatment: 1235898 INFO @ Wed, 22 Apr 2020 09:20:19: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Apr 2020 09:20:19: #1 finished! INFO @ Wed, 22 Apr 2020 09:20:19: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:20:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:20:19: #2 number of paired peaks: 44 WARNING @ Wed, 22 Apr 2020 09:20:19: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:20:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:20:24: 3000000 INFO @ Wed, 22 Apr 2020 09:20:32: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 09:20:40: 5000000 BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 09:20:46: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:20:46: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:20:46: #1 total tags in treatment: 2007180 INFO @ Wed, 22 Apr 2020 09:20:46: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:20:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:20:46: #1 tags after filtering in treatment: 1235898 INFO @ Wed, 22 Apr 2020 09:20:46: #1 Redundant rate of treatment: 0.38 INFO @ Wed, 22 Apr 2020 09:20:46: #1 finished! INFO @ Wed, 22 Apr 2020 09:20:46: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:20:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:20:46: #2 number of paired peaks: 44 WARNING @ Wed, 22 Apr 2020 09:20:46: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:20:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614993/SRX6614993.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling