Job ID = 5791196 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 9,109,368 reads read : 18,218,736 reads written : 18,218,736 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:53 9109368 reads; of these: 9109368 (100.00%) were paired; of these: 2873838 (31.55%) aligned concordantly 0 times 2080400 (22.84%) aligned concordantly exactly 1 time 4155130 (45.61%) aligned concordantly >1 times ---- 2873838 pairs aligned concordantly 0 times; of these: 320462 (11.15%) aligned discordantly 1 time ---- 2553376 pairs aligned 0 times concordantly or discordantly; of these: 5106752 mates make up the pairs; of these: 3687033 (72.20%) aligned 0 times 265687 (5.20%) aligned exactly 1 time 1154032 (22.60%) aligned >1 times 79.76% overall alignment rate Time searching: 00:07:53 Overall time: 00:07:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4173033 / 6495156 = 0.6425 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:14:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:14:08: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:14:08: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:14:15: 1000000 INFO @ Wed, 22 Apr 2020 09:14:21: 2000000 INFO @ Wed, 22 Apr 2020 09:14:28: 3000000 INFO @ Wed, 22 Apr 2020 09:14:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:14:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:14:38: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:14:38: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:14:40: 5000000 INFO @ Wed, 22 Apr 2020 09:14:45: 1000000 INFO @ Wed, 22 Apr 2020 09:14:47: 6000000 INFO @ Wed, 22 Apr 2020 09:14:48: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:14:48: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:14:48: #1 total tags in treatment: 2186392 INFO @ Wed, 22 Apr 2020 09:14:48: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:14:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:14:48: #1 tags after filtering in treatment: 1315665 INFO @ Wed, 22 Apr 2020 09:14:48: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 09:14:48: #1 finished! INFO @ Wed, 22 Apr 2020 09:14:48: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:14:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:14:48: #2 number of paired peaks: 51 WARNING @ Wed, 22 Apr 2020 09:14:48: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:14:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:14:51: 2000000 INFO @ Wed, 22 Apr 2020 09:14:58: 3000000 INFO @ Wed, 22 Apr 2020 09:15:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:15:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:15:08: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:15:08: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:15:10: 5000000 INFO @ Wed, 22 Apr 2020 09:15:15: 1000000 INFO @ Wed, 22 Apr 2020 09:15:17: 6000000 INFO @ Wed, 22 Apr 2020 09:15:19: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:15:19: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:15:19: #1 total tags in treatment: 2186392 INFO @ Wed, 22 Apr 2020 09:15:19: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:15:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:15:19: #1 tags after filtering in treatment: 1315665 INFO @ Wed, 22 Apr 2020 09:15:19: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 09:15:19: #1 finished! INFO @ Wed, 22 Apr 2020 09:15:19: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:15:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:15:19: #2 number of paired peaks: 51 WARNING @ Wed, 22 Apr 2020 09:15:19: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:15:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:15:22: 2000000 INFO @ Wed, 22 Apr 2020 09:15:29: 3000000 INFO @ Wed, 22 Apr 2020 09:15:35: 4000000 INFO @ Wed, 22 Apr 2020 09:15:41: 5000000 INFO @ Wed, 22 Apr 2020 09:15:48: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 09:15:49: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 09:15:49: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 09:15:49: #1 total tags in treatment: 2186392 INFO @ Wed, 22 Apr 2020 09:15:49: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:15:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:15:49: #1 tags after filtering in treatment: 1315665 INFO @ Wed, 22 Apr 2020 09:15:49: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 09:15:49: #1 finished! INFO @ Wed, 22 Apr 2020 09:15:49: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:15:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:15:49: #2 number of paired peaks: 51 WARNING @ Wed, 22 Apr 2020 09:15:49: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:15:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614987/SRX6614987.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。