Job ID = 5791172 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-21T23:46:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 6,571,716 reads read : 13,143,432 reads written : 13,143,432 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:30 6571716 reads; of these: 6571716 (100.00%) were paired; of these: 4199941 (63.91%) aligned concordantly 0 times 1984353 (30.20%) aligned concordantly exactly 1 time 387422 (5.90%) aligned concordantly >1 times ---- 4199941 pairs aligned concordantly 0 times; of these: 1971613 (46.94%) aligned discordantly 1 time ---- 2228328 pairs aligned 0 times concordantly or discordantly; of these: 4456656 mates make up the pairs; of these: 3666076 (82.26%) aligned 0 times 194942 (4.37%) aligned exactly 1 time 595638 (13.37%) aligned >1 times 72.11% overall alignment rate Time searching: 00:08:30 Overall time: 00:08:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 528815 / 4257255 = 0.1242 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:07:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:07:43: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:07:43: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:07:50: 1000000 INFO @ Wed, 22 Apr 2020 09:07:56: 2000000 INFO @ Wed, 22 Apr 2020 09:08:02: 3000000 INFO @ Wed, 22 Apr 2020 09:08:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:08:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:08:13: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:08:13: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:08:15: 5000000 INFO @ Wed, 22 Apr 2020 09:08:21: 6000000 INFO @ Wed, 22 Apr 2020 09:08:22: 1000000 INFO @ Wed, 22 Apr 2020 09:08:28: 7000000 INFO @ Wed, 22 Apr 2020 09:08:30: 2000000 INFO @ Wed, 22 Apr 2020 09:08:35: 8000000 INFO @ Wed, 22 Apr 2020 09:08:37: 3000000 INFO @ Wed, 22 Apr 2020 09:08:38: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:08:38: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:08:38: #1 total tags in treatment: 2076472 INFO @ Wed, 22 Apr 2020 09:08:38: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:08:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:08:38: #1 tags after filtering in treatment: 1618573 INFO @ Wed, 22 Apr 2020 09:08:38: #1 Redundant rate of treatment: 0.22 INFO @ Wed, 22 Apr 2020 09:08:38: #1 finished! INFO @ Wed, 22 Apr 2020 09:08:38: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:08:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:08:38: #2 number of paired peaks: 18 WARNING @ Wed, 22 Apr 2020 09:08:38: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:08:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:08:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:08:44: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:08:44: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:08:45: 4000000 INFO @ Wed, 22 Apr 2020 09:08:50: 1000000 INFO @ Wed, 22 Apr 2020 09:08:53: 5000000 INFO @ Wed, 22 Apr 2020 09:08:57: 2000000 INFO @ Wed, 22 Apr 2020 09:09:01: 6000000 INFO @ Wed, 22 Apr 2020 09:09:04: 3000000 INFO @ Wed, 22 Apr 2020 09:09:09: 7000000 INFO @ Wed, 22 Apr 2020 09:09:12: 4000000 INFO @ Wed, 22 Apr 2020 09:09:16: 8000000 INFO @ Wed, 22 Apr 2020 09:09:18: 5000000 INFO @ Wed, 22 Apr 2020 09:09:20: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:09:20: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:09:20: #1 total tags in treatment: 2076472 INFO @ Wed, 22 Apr 2020 09:09:20: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:09:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:09:20: #1 tags after filtering in treatment: 1618573 INFO @ Wed, 22 Apr 2020 09:09:20: #1 Redundant rate of treatment: 0.22 INFO @ Wed, 22 Apr 2020 09:09:20: #1 finished! INFO @ Wed, 22 Apr 2020 09:09:20: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:09:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:09:20: #2 number of paired peaks: 18 WARNING @ Wed, 22 Apr 2020 09:09:20: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:09:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:09:25: 6000000 INFO @ Wed, 22 Apr 2020 09:09:32: 7000000 INFO @ Wed, 22 Apr 2020 09:09:38: 8000000 INFO @ Wed, 22 Apr 2020 09:09:41: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:09:41: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:09:41: #1 total tags in treatment: 2076472 INFO @ Wed, 22 Apr 2020 09:09:41: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:09:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:09:41: #1 tags after filtering in treatment: 1618573 INFO @ Wed, 22 Apr 2020 09:09:41: #1 Redundant rate of treatment: 0.22 INFO @ Wed, 22 Apr 2020 09:09:41: #1 finished! INFO @ Wed, 22 Apr 2020 09:09:41: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:09:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:09:41: #2 number of paired peaks: 18 WARNING @ Wed, 22 Apr 2020 09:09:41: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:09:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614965/SRX6614965.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。