Job ID = 5791153 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 7,149,743 reads read : 14,299,486 reads written : 14,299,486 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:41 7149743 reads; of these: 7149743 (100.00%) were paired; of these: 3405420 (47.63%) aligned concordantly 0 times 979626 (13.70%) aligned concordantly exactly 1 time 2764697 (38.67%) aligned concordantly >1 times ---- 3405420 pairs aligned concordantly 0 times; of these: 545930 (16.03%) aligned discordantly 1 time ---- 2859490 pairs aligned 0 times concordantly or discordantly; of these: 5718980 mates make up the pairs; of these: 3043597 (53.22%) aligned 0 times 328627 (5.75%) aligned exactly 1 time 2346756 (41.03%) aligned >1 times 78.72% overall alignment rate Time searching: 00:09:41 Overall time: 00:09:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3254031 / 4224795 = 0.7702 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:04:52: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:04:52: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:05:00: 1000000 INFO @ Wed, 22 Apr 2020 09:05:08: 2000000 INFO @ Wed, 22 Apr 2020 09:05:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:05:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:05:21: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:05:21: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:05:24: 4000000 INFO @ Wed, 22 Apr 2020 09:05:31: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:05:31: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:05:31: #1 total tags in treatment: 812861 INFO @ Wed, 22 Apr 2020 09:05:31: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:05:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:05:31: #1 tags after filtering in treatment: 419269 INFO @ Wed, 22 Apr 2020 09:05:31: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Apr 2020 09:05:31: #1 finished! INFO @ Wed, 22 Apr 2020 09:05:31: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:05:31: #2 number of paired peaks: 168 WARNING @ Wed, 22 Apr 2020 09:05:31: Fewer paired peaks (168) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 168 pairs to build model! INFO @ Wed, 22 Apr 2020 09:05:31: start model_add_line... INFO @ Wed, 22 Apr 2020 09:05:31: start X-correlation... INFO @ Wed, 22 Apr 2020 09:05:31: end of X-cor INFO @ Wed, 22 Apr 2020 09:05:31: #2 finished! INFO @ Wed, 22 Apr 2020 09:05:31: #2 predicted fragment length is 214 bps INFO @ Wed, 22 Apr 2020 09:05:31: #2 alternative fragment length(s) may be 192,214 bps INFO @ Wed, 22 Apr 2020 09:05:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05_model.r WARNING @ Wed, 22 Apr 2020 09:05:31: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 09:05:31: #2 You may need to consider one of the other alternative d(s): 192,214 WARNING @ Wed, 22 Apr 2020 09:05:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 09:05:31: #3 Call peaks... INFO @ Wed, 22 Apr 2020 09:05:31: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 22 Apr 2020 09:05:32: 1000000 INFO @ Wed, 22 Apr 2020 09:05:32: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 09:05:33: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05_peaks.xls INFO @ Wed, 22 Apr 2020 09:05:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 09:05:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.05_summits.bed INFO @ Wed, 22 Apr 2020 09:05:33: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (246 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:05:42: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:05:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:05:51: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:05:51: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:05:52: 3000000 INFO @ Wed, 22 Apr 2020 09:06:03: 1000000 INFO @ Wed, 22 Apr 2020 09:06:05: 4000000 INFO @ Wed, 22 Apr 2020 09:06:12: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:06:12: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:06:12: #1 total tags in treatment: 812861 INFO @ Wed, 22 Apr 2020 09:06:12: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:06:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:06:12: #1 tags after filtering in treatment: 419269 INFO @ Wed, 22 Apr 2020 09:06:12: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Apr 2020 09:06:12: #1 finished! INFO @ Wed, 22 Apr 2020 09:06:12: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:06:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:06:12: #2 number of paired peaks: 168 WARNING @ Wed, 22 Apr 2020 09:06:12: Fewer paired peaks (168) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 168 pairs to build model! INFO @ Wed, 22 Apr 2020 09:06:12: start model_add_line... INFO @ Wed, 22 Apr 2020 09:06:12: start X-correlation... INFO @ Wed, 22 Apr 2020 09:06:12: end of X-cor INFO @ Wed, 22 Apr 2020 09:06:12: #2 finished! INFO @ Wed, 22 Apr 2020 09:06:12: #2 predicted fragment length is 214 bps INFO @ Wed, 22 Apr 2020 09:06:12: #2 alternative fragment length(s) may be 192,214 bps INFO @ Wed, 22 Apr 2020 09:06:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10_model.r WARNING @ Wed, 22 Apr 2020 09:06:12: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 09:06:12: #2 You may need to consider one of the other alternative d(s): 192,214 WARNING @ Wed, 22 Apr 2020 09:06:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 09:06:12: #3 Call peaks... INFO @ Wed, 22 Apr 2020 09:06:12: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 22 Apr 2020 09:06:13: 2000000 INFO @ Wed, 22 Apr 2020 09:06:14: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 09:06:14: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10_peaks.xls INFO @ Wed, 22 Apr 2020 09:06:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 09:06:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.10_summits.bed INFO @ Wed, 22 Apr 2020 09:06:14: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (115 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 09:06:23: 3000000 INFO @ Wed, 22 Apr 2020 09:06:33: 4000000 INFO @ Wed, 22 Apr 2020 09:06:41: #1 tag size is determined as 151 bps INFO @ Wed, 22 Apr 2020 09:06:41: #1 tag size = 151 INFO @ Wed, 22 Apr 2020 09:06:41: #1 total tags in treatment: 812861 INFO @ Wed, 22 Apr 2020 09:06:41: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:06:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:06:41: #1 tags after filtering in treatment: 419269 INFO @ Wed, 22 Apr 2020 09:06:41: #1 Redundant rate of treatment: 0.48 INFO @ Wed, 22 Apr 2020 09:06:41: #1 finished! INFO @ Wed, 22 Apr 2020 09:06:41: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:06:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:06:41: #2 number of paired peaks: 168 WARNING @ Wed, 22 Apr 2020 09:06:41: Fewer paired peaks (168) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 168 pairs to build model! INFO @ Wed, 22 Apr 2020 09:06:41: start model_add_line... INFO @ Wed, 22 Apr 2020 09:06:41: start X-correlation... INFO @ Wed, 22 Apr 2020 09:06:41: end of X-cor INFO @ Wed, 22 Apr 2020 09:06:41: #2 finished! INFO @ Wed, 22 Apr 2020 09:06:41: #2 predicted fragment length is 214 bps INFO @ Wed, 22 Apr 2020 09:06:41: #2 alternative fragment length(s) may be 192,214 bps INFO @ Wed, 22 Apr 2020 09:06:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20_model.r WARNING @ Wed, 22 Apr 2020 09:06:41: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 22 Apr 2020 09:06:41: #2 You may need to consider one of the other alternative d(s): 192,214 WARNING @ Wed, 22 Apr 2020 09:06:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 22 Apr 2020 09:06:41: #3 Call peaks... INFO @ Wed, 22 Apr 2020 09:06:41: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 22 Apr 2020 09:06:42: #3 Call peaks for each chromosome... INFO @ Wed, 22 Apr 2020 09:06:43: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20_peaks.xls INFO @ Wed, 22 Apr 2020 09:06:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20_peaks.narrowPeak INFO @ Wed, 22 Apr 2020 09:06:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX6614947/SRX6614947.20_summits.bed INFO @ Wed, 22 Apr 2020 09:06:43: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (44 records, 4 fields): 1 millis CompletedMACS2peakCalling