Job ID = 5791143 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 3,398,674 reads read : 6,797,348 reads written : 6,797,348 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 3398674 reads; of these: 3398674 (100.00%) were paired; of these: 2125589 (62.54%) aligned concordantly 0 times 574234 (16.90%) aligned concordantly exactly 1 time 698851 (20.56%) aligned concordantly >1 times ---- 2125589 pairs aligned concordantly 0 times; of these: 81319 (3.83%) aligned discordantly 1 time ---- 2044270 pairs aligned 0 times concordantly or discordantly; of these: 4088540 mates make up the pairs; of these: 3894518 (95.25%) aligned 0 times 37565 (0.92%) aligned exactly 1 time 156457 (3.83%) aligned >1 times 42.71% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 685857 / 1346989 = 0.5092 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:43:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:43:29: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:43:29: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:43:36: 1000000 INFO @ Wed, 22 Apr 2020 08:43:40: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:43:40: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:43:40: #1 total tags in treatment: 611151 INFO @ Wed, 22 Apr 2020 08:43:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:43:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:43:40: #1 tags after filtering in treatment: 373300 INFO @ Wed, 22 Apr 2020 08:43:40: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 08:43:40: #1 finished! INFO @ Wed, 22 Apr 2020 08:43:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:43:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:43:40: #2 number of paired peaks: 42 WARNING @ Wed, 22 Apr 2020 08:43:40: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:43:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:43:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:43:59: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:43:59: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:44:08: 1000000 INFO @ Wed, 22 Apr 2020 08:44:14: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:44:14: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:44:14: #1 total tags in treatment: 611151 INFO @ Wed, 22 Apr 2020 08:44:14: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:44:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:44:14: #1 tags after filtering in treatment: 373300 INFO @ Wed, 22 Apr 2020 08:44:14: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 08:44:14: #1 finished! INFO @ Wed, 22 Apr 2020 08:44:14: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:44:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:44:14: #2 number of paired peaks: 42 WARNING @ Wed, 22 Apr 2020 08:44:14: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:44:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:44:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:44:29: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:44:29: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:44:36: 1000000 INFO @ Wed, 22 Apr 2020 08:44:39: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:44:39: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:44:39: #1 total tags in treatment: 611151 INFO @ Wed, 22 Apr 2020 08:44:39: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:44:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:44:39: #1 tags after filtering in treatment: 373300 INFO @ Wed, 22 Apr 2020 08:44:39: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 08:44:39: #1 finished! INFO @ Wed, 22 Apr 2020 08:44:39: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:44:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:44:39: #2 number of paired peaks: 42 WARNING @ Wed, 22 Apr 2020 08:44:39: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:44:39: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6614936/SRX6614936.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。