Job ID = 7098660 SRX = SRX6473466 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 2018232 spots for SRR9715589/SRR9715589.sra Written 2018232 spots for SRR9715589/SRR9715589.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 2018232 reads; of these: 2018232 (100.00%) were unpaired; of these: 114609 (5.68%) aligned 0 times 1619804 (80.26%) aligned exactly 1 time 283819 (14.06%) aligned >1 times 94.32% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 199969 / 1903623 = 0.1050 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:59:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:59:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:00:00: 1000000 INFO @ Wed, 22 Jul 2020 12:00:04: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:00:04: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:00:04: #1 total tags in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:00:04: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:00:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:00:04: #1 tags after filtering in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:00:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:00:04: #1 finished! INFO @ Wed, 22 Jul 2020 12:00:04: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:00:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:00:04: #2 number of paired peaks: 31 WARNING @ Wed, 22 Jul 2020 12:00:04: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:00:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:00:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:00:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:00:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:00:30: 1000000 INFO @ Wed, 22 Jul 2020 12:00:34: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:00:34: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:00:34: #1 total tags in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:00:34: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:00:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:00:34: #1 tags after filtering in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:00:34: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:00:34: #1 finished! INFO @ Wed, 22 Jul 2020 12:00:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:00:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:00:34: #2 number of paired peaks: 31 WARNING @ Wed, 22 Jul 2020 12:00:34: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:00:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:00:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:00:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:00:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:01:00: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:01:04: #1 tag size is determined as 101 bps INFO @ Wed, 22 Jul 2020 12:01:04: #1 tag size = 101 INFO @ Wed, 22 Jul 2020 12:01:04: #1 total tags in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:01:04: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:01:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:01:04: #1 tags after filtering in treatment: 1703654 INFO @ Wed, 22 Jul 2020 12:01:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:01:04: #1 finished! INFO @ Wed, 22 Jul 2020 12:01:04: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:01:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:01:04: #2 number of paired peaks: 31 WARNING @ Wed, 22 Jul 2020 12:01:04: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:01:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6473466/SRX6473466.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。