Job ID = 9163156 sra ファイルのダウンロード中... Completed: 750621K bytes transferred in 9 seconds (683147K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21510449 spots for /home/okishinya/chipatlas/results/sacCer3/SRX643290/SRR1503349.sra Written 21510449 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:48 21510449 reads; of these: 21510449 (100.00%) were unpaired; of these: 1273927 (5.92%) aligned 0 times 17632925 (81.97%) aligned exactly 1 time 2603597 (12.10%) aligned >1 times 94.08% overall alignment rate Time searching: 00:03:48 Overall time: 00:03:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9111638 / 20236522 = 0.4503 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 10:17:50: # Command line: callpeak -t SRX643290.bam -f BAM -g 12100000 -n SRX643290.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX643290.10 # format = BAM # ChIP-seq file = ['SRX643290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:17:50: # Command line: callpeak -t SRX643290.bam -f BAM -g 12100000 -n SRX643290.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX643290.05 # format = BAM # ChIP-seq file = ['SRX643290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:17:50: # Command line: callpeak -t SRX643290.bam -f BAM -g 12100000 -n SRX643290.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX643290.20 # format = BAM # ChIP-seq file = ['SRX643290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:17:56: 1000000 INFO @ Wed, 28 Jun 2017 10:17:56: 1000000 INFO @ Wed, 28 Jun 2017 10:17:56: 1000000 INFO @ Wed, 28 Jun 2017 10:18:02: 2000000 INFO @ Wed, 28 Jun 2017 10:18:02: 2000000 INFO @ Wed, 28 Jun 2017 10:18:02: 2000000 INFO @ Wed, 28 Jun 2017 10:18:09: 3000000 INFO @ Wed, 28 Jun 2017 10:18:09: 3000000 INFO @ Wed, 28 Jun 2017 10:18:09: 3000000 INFO @ Wed, 28 Jun 2017 10:18:15: 4000000 INFO @ Wed, 28 Jun 2017 10:18:15: 4000000 INFO @ Wed, 28 Jun 2017 10:18:15: 4000000 INFO @ Wed, 28 Jun 2017 10:18:21: 5000000 INFO @ Wed, 28 Jun 2017 10:18:21: 5000000 INFO @ Wed, 28 Jun 2017 10:18:22: 5000000 INFO @ Wed, 28 Jun 2017 10:18:27: 6000000 INFO @ Wed, 28 Jun 2017 10:18:28: 6000000 INFO @ Wed, 28 Jun 2017 10:18:28: 6000000 INFO @ Wed, 28 Jun 2017 10:18:34: 7000000 INFO @ Wed, 28 Jun 2017 10:18:34: 7000000 INFO @ Wed, 28 Jun 2017 10:18:34: 7000000 INFO @ Wed, 28 Jun 2017 10:18:40: 8000000 INFO @ Wed, 28 Jun 2017 10:18:40: 8000000 INFO @ Wed, 28 Jun 2017 10:18:41: 8000000 INFO @ Wed, 28 Jun 2017 10:18:46: 9000000 INFO @ Wed, 28 Jun 2017 10:18:47: 9000000 INFO @ Wed, 28 Jun 2017 10:18:47: 9000000 INFO @ Wed, 28 Jun 2017 10:18:52: 10000000 INFO @ Wed, 28 Jun 2017 10:18:53: 10000000 INFO @ Wed, 28 Jun 2017 10:18:54: 10000000 INFO @ Wed, 28 Jun 2017 10:18:58: 11000000 INFO @ Wed, 28 Jun 2017 10:18:59: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 10:18:59: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 10:18:59: #1 total tags in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:18:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:18:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:19:00: #1 tags after filtering in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:19:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:19:00: #1 finished! INFO @ Wed, 28 Jun 2017 10:19:00: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:19:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:19:00: 11000000 INFO @ Wed, 28 Jun 2017 10:19:00: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:19:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:19:00: Process for pairing-model is terminated! cat: SRX643290.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX643290.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:19:00: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 10:19:00: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 10:19:00: #1 total tags in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:19:00: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:19:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:19:01: 11000000 INFO @ Wed, 28 Jun 2017 10:19:01: #1 tags after filtering in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:19:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:19:01: #1 finished! INFO @ Wed, 28 Jun 2017 10:19:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:19:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:19:01: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:19:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:19:01: Process for pairing-model is terminated! cat: SRX643290.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX643290.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:19:01: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 10:19:01: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 10:19:01: #1 total tags in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:19:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:19:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:19:02: #1 tags after filtering in treatment: 11124884 INFO @ Wed, 28 Jun 2017 10:19:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:19:02: #1 finished! INFO @ Wed, 28 Jun 2017 10:19:02: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:19:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:19:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:19:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:19:02: Process for pairing-model is terminated! cat: SRX643290.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX643290.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX643290.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。