Job ID = 5791116 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 23,245,939 reads read : 46,491,878 reads written : 46,491,878 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:34 23245939 reads; of these: 23245939 (100.00%) were paired; of these: 2641358 (11.36%) aligned concordantly 0 times 17911721 (77.05%) aligned concordantly exactly 1 time 2692860 (11.58%) aligned concordantly >1 times ---- 2641358 pairs aligned concordantly 0 times; of these: 497668 (18.84%) aligned discordantly 1 time ---- 2143690 pairs aligned 0 times concordantly or discordantly; of these: 4287380 mates make up the pairs; of these: 3451573 (80.51%) aligned 0 times 446976 (10.43%) aligned exactly 1 time 388831 (9.07%) aligned >1 times 92.58% overall alignment rate Time searching: 00:11:34 Overall time: 00:11:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2964574 / 21071919 = 0.1407 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:09:14: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:09:14: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:09:20: 1000000 INFO @ Wed, 22 Apr 2020 09:09:26: 2000000 INFO @ Wed, 22 Apr 2020 09:09:32: 3000000 INFO @ Wed, 22 Apr 2020 09:09:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:09:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:09:44: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:09:44: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:09:45: 5000000 INFO @ Wed, 22 Apr 2020 09:09:50: 1000000 INFO @ Wed, 22 Apr 2020 09:09:52: 6000000 INFO @ Wed, 22 Apr 2020 09:09:57: 2000000 INFO @ Wed, 22 Apr 2020 09:09:59: 7000000 INFO @ Wed, 22 Apr 2020 09:10:04: 3000000 INFO @ Wed, 22 Apr 2020 09:10:06: 8000000 INFO @ Wed, 22 Apr 2020 09:10:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:10:12: 9000000 INFO @ Wed, 22 Apr 2020 09:10:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:10:14: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:10:14: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:10:17: 5000000 INFO @ Wed, 22 Apr 2020 09:10:19: 10000000 INFO @ Wed, 22 Apr 2020 09:10:20: 1000000 INFO @ Wed, 22 Apr 2020 09:10:24: 6000000 INFO @ Wed, 22 Apr 2020 09:10:26: 11000000 INFO @ Wed, 22 Apr 2020 09:10:26: 2000000 INFO @ Wed, 22 Apr 2020 09:10:30: 7000000 INFO @ Wed, 22 Apr 2020 09:10:32: 3000000 INFO @ Wed, 22 Apr 2020 09:10:33: 12000000 INFO @ Wed, 22 Apr 2020 09:10:37: 8000000 INFO @ Wed, 22 Apr 2020 09:10:38: 4000000 INFO @ Wed, 22 Apr 2020 09:10:40: 13000000 INFO @ Wed, 22 Apr 2020 09:10:44: 9000000 INFO @ Wed, 22 Apr 2020 09:10:44: 5000000 INFO @ Wed, 22 Apr 2020 09:10:46: 14000000 INFO @ Wed, 22 Apr 2020 09:10:50: 10000000 INFO @ Wed, 22 Apr 2020 09:10:50: 6000000 INFO @ Wed, 22 Apr 2020 09:10:53: 15000000 INFO @ Wed, 22 Apr 2020 09:10:57: 7000000 INFO @ Wed, 22 Apr 2020 09:10:57: 11000000 INFO @ Wed, 22 Apr 2020 09:11:00: 16000000 INFO @ Wed, 22 Apr 2020 09:11:03: 8000000 INFO @ Wed, 22 Apr 2020 09:11:04: 12000000 INFO @ Wed, 22 Apr 2020 09:11:07: 17000000 INFO @ Wed, 22 Apr 2020 09:11:09: 9000000 INFO @ Wed, 22 Apr 2020 09:11:11: 13000000 INFO @ Wed, 22 Apr 2020 09:11:14: 18000000 INFO @ Wed, 22 Apr 2020 09:11:15: 10000000 INFO @ Wed, 22 Apr 2020 09:11:17: 14000000 INFO @ Wed, 22 Apr 2020 09:11:20: 19000000 INFO @ Wed, 22 Apr 2020 09:11:21: 11000000 INFO @ Wed, 22 Apr 2020 09:11:24: 15000000 INFO @ Wed, 22 Apr 2020 09:11:27: 12000000 INFO @ Wed, 22 Apr 2020 09:11:27: 20000000 INFO @ Wed, 22 Apr 2020 09:11:31: 16000000 INFO @ Wed, 22 Apr 2020 09:11:33: 13000000 INFO @ Wed, 22 Apr 2020 09:11:34: 21000000 INFO @ Wed, 22 Apr 2020 09:11:37: 17000000 INFO @ Wed, 22 Apr 2020 09:11:39: 14000000 INFO @ Wed, 22 Apr 2020 09:11:41: 22000000 INFO @ Wed, 22 Apr 2020 09:11:44: 18000000 INFO @ Wed, 22 Apr 2020 09:11:45: 15000000 INFO @ Wed, 22 Apr 2020 09:11:48: 23000000 INFO @ Wed, 22 Apr 2020 09:11:51: 16000000 INFO @ Wed, 22 Apr 2020 09:11:51: 19000000 INFO @ Wed, 22 Apr 2020 09:11:54: 24000000 INFO @ Wed, 22 Apr 2020 09:11:57: 17000000 INFO @ Wed, 22 Apr 2020 09:11:58: 20000000 INFO @ Wed, 22 Apr 2020 09:12:01: 25000000 INFO @ Wed, 22 Apr 2020 09:12:03: 18000000 INFO @ Wed, 22 Apr 2020 09:12:04: 21000000 INFO @ Wed, 22 Apr 2020 09:12:08: 26000000 INFO @ Wed, 22 Apr 2020 09:12:09: 19000000 INFO @ Wed, 22 Apr 2020 09:12:11: 22000000 INFO @ Wed, 22 Apr 2020 09:12:15: 27000000 INFO @ Wed, 22 Apr 2020 09:12:16: 20000000 INFO @ Wed, 22 Apr 2020 09:12:18: 23000000 INFO @ Wed, 22 Apr 2020 09:12:21: 28000000 INFO @ Wed, 22 Apr 2020 09:12:22: 21000000 INFO @ Wed, 22 Apr 2020 09:12:24: 24000000 INFO @ Wed, 22 Apr 2020 09:12:28: 29000000 INFO @ Wed, 22 Apr 2020 09:12:28: 22000000 INFO @ Wed, 22 Apr 2020 09:12:31: 25000000 INFO @ Wed, 22 Apr 2020 09:12:34: 23000000 INFO @ Wed, 22 Apr 2020 09:12:34: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 09:12:39: 26000000 INFO @ Wed, 22 Apr 2020 09:12:40: 24000000 INFO @ Wed, 22 Apr 2020 09:12:41: 31000000 INFO @ Wed, 22 Apr 2020 09:12:46: 27000000 INFO @ Wed, 22 Apr 2020 09:12:46: 25000000 INFO @ Wed, 22 Apr 2020 09:12:48: 32000000 INFO @ Wed, 22 Apr 2020 09:12:53: 26000000 INFO @ Wed, 22 Apr 2020 09:12:53: 28000000 BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 09:12:54: 33000000 INFO @ Wed, 22 Apr 2020 09:12:59: 27000000 INFO @ Wed, 22 Apr 2020 09:13:00: 29000000 INFO @ Wed, 22 Apr 2020 09:13:01: 34000000 INFO @ Wed, 22 Apr 2020 09:13:05: 28000000 INFO @ Wed, 22 Apr 2020 09:13:06: 30000000 INFO @ Wed, 22 Apr 2020 09:13:07: 35000000 INFO @ Wed, 22 Apr 2020 09:13:11: 29000000 INFO @ Wed, 22 Apr 2020 09:13:13: 31000000 INFO @ Wed, 22 Apr 2020 09:13:14: 36000000 INFO @ Wed, 22 Apr 2020 09:13:17: 30000000 INFO @ Wed, 22 Apr 2020 09:13:20: 32000000 INFO @ Wed, 22 Apr 2020 09:13:20: 37000000 INFO @ Wed, 22 Apr 2020 09:13:21: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 09:13:21: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 09:13:21: #1 total tags in treatment: 17668286 INFO @ Wed, 22 Apr 2020 09:13:21: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:13:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:13:21: #1 tags after filtering in treatment: 11330516 INFO @ Wed, 22 Apr 2020 09:13:21: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Apr 2020 09:13:21: #1 finished! INFO @ Wed, 22 Apr 2020 09:13:21: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:13:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:13:22: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:13:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:13:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:13:23: 31000000 INFO @ Wed, 22 Apr 2020 09:13:26: 33000000 INFO @ Wed, 22 Apr 2020 09:13:29: 32000000 INFO @ Wed, 22 Apr 2020 09:13:33: 34000000 INFO @ Wed, 22 Apr 2020 09:13:35: 33000000 INFO @ Wed, 22 Apr 2020 09:13:39: 35000000 INFO @ Wed, 22 Apr 2020 09:13:40: 34000000 INFO @ Wed, 22 Apr 2020 09:13:46: 36000000 INFO @ Wed, 22 Apr 2020 09:13:46: 35000000 INFO @ Wed, 22 Apr 2020 09:13:52: 36000000 INFO @ Wed, 22 Apr 2020 09:13:52: 37000000 INFO @ Wed, 22 Apr 2020 09:13:53: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 09:13:53: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 09:13:53: #1 total tags in treatment: 17668286 INFO @ Wed, 22 Apr 2020 09:13:53: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:13:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:13:54: #1 tags after filtering in treatment: 11330516 INFO @ Wed, 22 Apr 2020 09:13:54: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Apr 2020 09:13:54: #1 finished! INFO @ Wed, 22 Apr 2020 09:13:54: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:13:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:13:54: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:13:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:13:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 09:13:58: 37000000 INFO @ Wed, 22 Apr 2020 09:13:58: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 09:13:58: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 09:13:58: #1 total tags in treatment: 17668286 INFO @ Wed, 22 Apr 2020 09:13:58: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:13:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:13:59: #1 tags after filtering in treatment: 11330516 INFO @ Wed, 22 Apr 2020 09:13:59: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Apr 2020 09:13:59: #1 finished! INFO @ Wed, 22 Apr 2020 09:13:59: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:13:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:13:59: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 09:13:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:13:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6078427/SRX6078427.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling