Job ID = 5791108 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,726,906 reads read : 11,453,812 reads written : 5,726,906 reads 0-length : 5,726,906 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 5726906 reads; of these: 5726906 (100.00%) were unpaired; of these: 430973 (7.53%) aligned 0 times 4601778 (80.35%) aligned exactly 1 time 694155 (12.12%) aligned >1 times 92.47% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1038621 / 5295933 = 0.1961 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:37:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:37:34: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:37:41: 1000000 INFO @ Wed, 22 Apr 2020 08:37:48: 2000000 INFO @ Wed, 22 Apr 2020 08:37:55: 3000000 INFO @ Wed, 22 Apr 2020 08:38:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:38:03: #1 tag size is determined as 100 bps INFO @ Wed, 22 Apr 2020 08:38:03: #1 tag size = 100 INFO @ Wed, 22 Apr 2020 08:38:03: #1 total tags in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:38:03: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:38:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:38:03: #1 tags after filtering in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:38:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:38:03: #1 finished! INFO @ Wed, 22 Apr 2020 08:38:03: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:38:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:38:04: #2 number of paired peaks: 36 WARNING @ Wed, 22 Apr 2020 08:38:04: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:38:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:38:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:38:04: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:38:04: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:38:11: 1000000 INFO @ Wed, 22 Apr 2020 08:38:19: 2000000 INFO @ Wed, 22 Apr 2020 08:38:26: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:38:33: 4000000 INFO @ Wed, 22 Apr 2020 08:38:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:38:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:38:34: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:38:35: #1 tag size is determined as 100 bps INFO @ Wed, 22 Apr 2020 08:38:35: #1 tag size = 100 INFO @ Wed, 22 Apr 2020 08:38:35: #1 total tags in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:38:35: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:38:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:38:35: #1 tags after filtering in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:38:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:38:35: #1 finished! INFO @ Wed, 22 Apr 2020 08:38:35: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:38:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:38:35: #2 number of paired peaks: 36 WARNING @ Wed, 22 Apr 2020 08:38:35: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:38:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:38:42: 1000000 INFO @ Wed, 22 Apr 2020 08:38:49: 2000000 INFO @ Wed, 22 Apr 2020 08:38:56: 3000000 INFO @ Wed, 22 Apr 2020 08:39:02: 4000000 INFO @ Wed, 22 Apr 2020 08:39:04: #1 tag size is determined as 100 bps INFO @ Wed, 22 Apr 2020 08:39:04: #1 tag size = 100 INFO @ Wed, 22 Apr 2020 08:39:04: #1 total tags in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:39:04: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:39:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:39:04: #1 tags after filtering in treatment: 4257312 INFO @ Wed, 22 Apr 2020 08:39:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:39:04: #1 finished! INFO @ Wed, 22 Apr 2020 08:39:04: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:39:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:39:04: #2 number of paired peaks: 36 WARNING @ Wed, 22 Apr 2020 08:39:04: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:39:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936176/SRX5936176.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。