Job ID = 5791097 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,660,065 reads read : 15,320,130 reads written : 7,660,065 reads 0-length : 7,660,065 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:53 7660065 reads; of these: 7660065 (100.00%) were unpaired; of these: 849671 (11.09%) aligned 0 times 5916573 (77.24%) aligned exactly 1 time 893821 (11.67%) aligned >1 times 88.91% overall alignment rate Time searching: 00:00:53 Overall time: 00:00:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1663222 / 6810394 = 0.2442 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:33:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:33:08: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:33:08: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:33:14: 1000000 INFO @ Wed, 22 Apr 2020 08:33:21: 2000000 INFO @ Wed, 22 Apr 2020 08:33:27: 3000000 INFO @ Wed, 22 Apr 2020 08:33:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:33:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:33:38: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:33:38: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:33:40: 5000000 INFO @ Wed, 22 Apr 2020 08:33:41: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:33:41: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:33:41: #1 total tags in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:33:41: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:33:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:33:41: #1 tags after filtering in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:33:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:33:41: #1 finished! INFO @ Wed, 22 Apr 2020 08:33:41: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:33:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:33:41: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:33:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:33:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:33:45: 1000000 INFO @ Wed, 22 Apr 2020 08:33:51: 2000000 INFO @ Wed, 22 Apr 2020 08:33:58: 3000000 INFO @ Wed, 22 Apr 2020 08:34:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:34:08: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:34:08: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:34:11: 5000000 INFO @ Wed, 22 Apr 2020 08:34:12: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:34:12: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:34:12: #1 total tags in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:34:12: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:34:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:34:12: #1 tags after filtering in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:34:12: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:34:12: #1 finished! INFO @ Wed, 22 Apr 2020 08:34:12: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:34:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:34:12: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:34:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:34:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:34:15: 1000000 INFO @ Wed, 22 Apr 2020 08:34:22: 2000000 INFO @ Wed, 22 Apr 2020 08:34:29: 3000000 INFO @ Wed, 22 Apr 2020 08:34:35: 4000000 INFO @ Wed, 22 Apr 2020 08:34:42: 5000000 INFO @ Wed, 22 Apr 2020 08:34:42: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:34:42: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:34:42: #1 total tags in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:34:42: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:34:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:34:42: #1 tags after filtering in treatment: 5147172 INFO @ Wed, 22 Apr 2020 08:34:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:34:42: #1 finished! INFO @ Wed, 22 Apr 2020 08:34:42: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:34:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:34:43: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:34:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936165/SRX5936165.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。