Job ID = 5791096 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,182,732 reads read : 16,365,464 reads written : 8,182,732 reads 0-length : 8,182,732 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:06 8182732 reads; of these: 8182732 (100.00%) were unpaired; of these: 626464 (7.66%) aligned 0 times 6526757 (79.76%) aligned exactly 1 time 1029511 (12.58%) aligned >1 times 92.34% overall alignment rate Time searching: 00:01:06 Overall time: 00:01:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2263378 / 7556268 = 0.2995 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:32:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:32:59: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:32:59: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:33:05: 1000000 INFO @ Wed, 22 Apr 2020 08:33:10: 2000000 INFO @ Wed, 22 Apr 2020 08:33:15: 3000000 INFO @ Wed, 22 Apr 2020 08:33:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:33:26: 5000000 INFO @ Wed, 22 Apr 2020 08:33:27: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:33:27: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:33:27: #1 total tags in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:33:27: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:33:27: #1 tags after filtering in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:33:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:33:27: #1 finished! INFO @ Wed, 22 Apr 2020 08:33:27: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:33:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:33:27: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:33:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:33:27: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 08:33:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:33:28: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:33:28: #1 read treatment tags... cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:33:34: 1000000 INFO @ Wed, 22 Apr 2020 08:33:39: 2000000 INFO @ Wed, 22 Apr 2020 08:33:44: 3000000 INFO @ Wed, 22 Apr 2020 08:33:50: 4000000 INFO @ Wed, 22 Apr 2020 08:33:55: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:33:56: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:33:56: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:33:56: #1 total tags in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:33:56: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:33:56: #1 tags after filtering in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:33:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:33:56: #1 finished! INFO @ Wed, 22 Apr 2020 08:33:56: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:33:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:33:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:33:56: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 08:33:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:33:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:33:58: #1 read treatment tags... cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:34:03: 1000000 INFO @ Wed, 22 Apr 2020 08:34:08: 2000000 INFO @ Wed, 22 Apr 2020 08:34:13: 3000000 INFO @ Wed, 22 Apr 2020 08:34:19: 4000000 INFO @ Wed, 22 Apr 2020 08:34:24: 5000000 INFO @ Wed, 22 Apr 2020 08:34:25: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 08:34:25: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 08:34:25: #1 total tags in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:34:25: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:34:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:34:25: #1 tags after filtering in treatment: 5292890 INFO @ Wed, 22 Apr 2020 08:34:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:34:25: #1 finished! INFO @ Wed, 22 Apr 2020 08:34:25: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:34:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:34:26: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 08:34:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:34:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936164/SRX5936164.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。