Job ID = 5791084 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,500,990 reads read : 13,001,980 reads written : 6,500,990 reads 0-length : 6,500,990 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:36 6500990 reads; of these: 6500990 (100.00%) were unpaired; of these: 418171 (6.43%) aligned 0 times 5313036 (81.73%) aligned exactly 1 time 769783 (11.84%) aligned >1 times 93.57% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1283008 / 6082819 = 0.2109 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:35:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:35:05: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:35:05: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:35:11: 1000000 INFO @ Wed, 22 Apr 2020 08:35:16: 2000000 INFO @ Wed, 22 Apr 2020 08:35:22: 3000000 INFO @ Wed, 22 Apr 2020 08:35:27: 4000000 INFO @ Wed, 22 Apr 2020 08:35:32: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:35:32: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:35:32: #1 total tags in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:35:32: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:35:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:35:32: #1 tags after filtering in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:35:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:35:32: #1 finished! INFO @ Wed, 22 Apr 2020 08:35:32: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:35:32: #2 looking for paired plus/minus strand peaks... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:35:32: #2 number of paired peaks: 19 WARNING @ Wed, 22 Apr 2020 08:35:32: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:35:32: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 08:35:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:35:35: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:35:35: #1 read treatment tags... cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:35:40: 1000000 INFO @ Wed, 22 Apr 2020 08:35:45: 2000000 INFO @ Wed, 22 Apr 2020 08:35:51: 3000000 INFO @ Wed, 22 Apr 2020 08:35:56: 4000000 INFO @ Wed, 22 Apr 2020 08:36:00: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:36:00: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:36:00: #1 total tags in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:36:00: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:36:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:36:01: #1 tags after filtering in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:36:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:36:01: #1 finished! INFO @ Wed, 22 Apr 2020 08:36:01: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:36:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:36:01: #2 number of paired peaks: 19 WARNING @ Wed, 22 Apr 2020 08:36:01: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:36:01: Process for pairing-model is terminated! BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:36:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:36:06: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:36:06: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:36:12: 1000000 INFO @ Wed, 22 Apr 2020 08:36:19: 2000000 INFO @ Wed, 22 Apr 2020 08:36:26: 3000000 INFO @ Wed, 22 Apr 2020 08:36:32: 4000000 INFO @ Wed, 22 Apr 2020 08:36:38: #1 tag size is determined as 101 bps INFO @ Wed, 22 Apr 2020 08:36:38: #1 tag size = 101 INFO @ Wed, 22 Apr 2020 08:36:38: #1 total tags in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:36:38: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:36:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:36:38: #1 tags after filtering in treatment: 4799811 INFO @ Wed, 22 Apr 2020 08:36:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:36:38: #1 finished! INFO @ Wed, 22 Apr 2020 08:36:38: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:36:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:36:38: #2 number of paired peaks: 19 WARNING @ Wed, 22 Apr 2020 08:36:38: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:36:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936153/SRX5936153.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。