Job ID = 5791083 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,675,544 reads read : 13,351,088 reads written : 6,675,544 reads 0-length : 6,675,544 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:11 6675544 reads; of these: 6675544 (100.00%) were unpaired; of these: 537045 (8.04%) aligned 0 times 5149589 (77.14%) aligned exactly 1 time 988910 (14.81%) aligned >1 times 91.96% overall alignment rate Time searching: 00:01:11 Overall time: 00:01:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1712864 / 6138499 = 0.2790 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:33:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:33:44: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:33:44: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:33:52: 1000000 INFO @ Wed, 22 Apr 2020 08:34:00: 2000000 INFO @ Wed, 22 Apr 2020 08:34:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:34:14: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:34:14: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:34:16: 4000000 INFO @ Wed, 22 Apr 2020 08:34:20: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 08:34:20: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 08:34:20: #1 total tags in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:34:20: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:34:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:34:20: #1 tags after filtering in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:34:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:34:20: #1 finished! INFO @ Wed, 22 Apr 2020 08:34:20: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:34:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:34:20: #2 number of paired peaks: 26 WARNING @ Wed, 22 Apr 2020 08:34:20: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:34:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:34:23: 1000000 INFO @ Wed, 22 Apr 2020 08:34:29: 2000000 INFO @ Wed, 22 Apr 2020 08:34:36: 3000000 INFO @ Wed, 22 Apr 2020 08:34:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:34:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:34:44: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:34:44: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:34:45: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 08:34:45: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 08:34:45: #1 total tags in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:34:45: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:34:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:34:45: #1 tags after filtering in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:34:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:34:45: #1 finished! INFO @ Wed, 22 Apr 2020 08:34:45: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:34:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:34:45: #2 number of paired peaks: 26 WARNING @ Wed, 22 Apr 2020 08:34:45: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:34:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:34:52: 1000000 INFO @ Wed, 22 Apr 2020 08:35:00: 2000000 INFO @ Wed, 22 Apr 2020 08:35:07: 3000000 INFO @ Wed, 22 Apr 2020 08:35:15: 4000000 INFO @ Wed, 22 Apr 2020 08:35:18: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 08:35:18: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 08:35:18: #1 total tags in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:35:18: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:35:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:35:18: #1 tags after filtering in treatment: 4425635 INFO @ Wed, 22 Apr 2020 08:35:18: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 08:35:18: #1 finished! INFO @ Wed, 22 Apr 2020 08:35:18: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:35:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:35:19: #2 number of paired peaks: 26 WARNING @ Wed, 22 Apr 2020 08:35:19: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:35:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5936152/SRX5936152.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。