Job ID = 5791051 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 3,250,285 reads read : 6,500,570 reads written : 6,500,570 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:17 3250285 reads; of these: 3250285 (100.00%) were paired; of these: 699848 (21.53%) aligned concordantly 0 times 2206427 (67.88%) aligned concordantly exactly 1 time 344010 (10.58%) aligned concordantly >1 times ---- 699848 pairs aligned concordantly 0 times; of these: 130201 (18.60%) aligned discordantly 1 time ---- 569647 pairs aligned 0 times concordantly or discordantly; of these: 1139294 mates make up the pairs; of these: 870572 (76.41%) aligned 0 times 204129 (17.92%) aligned exactly 1 time 64593 (5.67%) aligned >1 times 86.61% overall alignment rate Time searching: 00:01:17 Overall time: 00:01:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 98294 / 2655282 = 0.0370 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:23:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:23:46: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:23:46: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:23:50: 1000000 INFO @ Wed, 22 Apr 2020 08:23:54: 2000000 INFO @ Wed, 22 Apr 2020 08:23:58: 3000000 INFO @ Wed, 22 Apr 2020 08:24:01: 4000000 INFO @ Wed, 22 Apr 2020 08:24:05: 5000000 INFO @ Wed, 22 Apr 2020 08:24:07: #1 tag size is determined as 38 bps INFO @ Wed, 22 Apr 2020 08:24:07: #1 tag size = 38 INFO @ Wed, 22 Apr 2020 08:24:07: #1 total tags in treatment: 2457478 INFO @ Wed, 22 Apr 2020 08:24:07: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:24:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:24:07: #1 tags after filtering in treatment: 2013109 INFO @ Wed, 22 Apr 2020 08:24:07: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 22 Apr 2020 08:24:07: #1 finished! INFO @ Wed, 22 Apr 2020 08:24:07: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:24:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:24:07: #2 number of paired peaks: 30 WARNING @ Wed, 22 Apr 2020 08:24:07: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:24:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:24:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:24:16: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:24:16: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:24:21: 1000000 INFO @ Wed, 22 Apr 2020 08:24:25: 2000000 INFO @ Wed, 22 Apr 2020 08:24:30: 3000000 INFO @ Wed, 22 Apr 2020 08:24:34: 4000000 INFO @ Wed, 22 Apr 2020 08:24:38: 5000000 INFO @ Wed, 22 Apr 2020 08:24:40: #1 tag size is determined as 38 bps INFO @ Wed, 22 Apr 2020 08:24:40: #1 tag size = 38 INFO @ Wed, 22 Apr 2020 08:24:40: #1 total tags in treatment: 2457478 INFO @ Wed, 22 Apr 2020 08:24:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:24:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:24:40: #1 tags after filtering in treatment: 2013109 INFO @ Wed, 22 Apr 2020 08:24:40: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 22 Apr 2020 08:24:40: #1 finished! INFO @ Wed, 22 Apr 2020 08:24:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:24:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:24:40: #2 number of paired peaks: 30 WARNING @ Wed, 22 Apr 2020 08:24:40: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:24:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:24:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:24:46: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:24:46: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:24:51: 1000000 INFO @ Wed, 22 Apr 2020 08:24:55: 2000000 INFO @ Wed, 22 Apr 2020 08:25:00: 3000000 INFO @ Wed, 22 Apr 2020 08:25:04: 4000000 INFO @ Wed, 22 Apr 2020 08:25:09: 5000000 INFO @ Wed, 22 Apr 2020 08:25:11: #1 tag size is determined as 38 bps INFO @ Wed, 22 Apr 2020 08:25:11: #1 tag size = 38 INFO @ Wed, 22 Apr 2020 08:25:11: #1 total tags in treatment: 2457478 INFO @ Wed, 22 Apr 2020 08:25:11: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:25:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:25:11: #1 tags after filtering in treatment: 2013109 INFO @ Wed, 22 Apr 2020 08:25:11: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 22 Apr 2020 08:25:11: #1 finished! INFO @ Wed, 22 Apr 2020 08:25:11: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:25:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:25:11: #2 number of paired peaks: 30 WARNING @ Wed, 22 Apr 2020 08:25:11: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:25:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5883275/SRX5883275.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。