Job ID = 5790986 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 2,182,318 reads read : 4,364,636 reads written : 4,364,636 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:41 2182318 reads; of these: 2182318 (100.00%) were paired; of these: 114355 (5.24%) aligned concordantly 0 times 1879672 (86.13%) aligned concordantly exactly 1 time 188291 (8.63%) aligned concordantly >1 times ---- 114355 pairs aligned concordantly 0 times; of these: 1991 (1.74%) aligned discordantly 1 time ---- 112364 pairs aligned 0 times concordantly or discordantly; of these: 224728 mates make up the pairs; of these: 211024 (93.90%) aligned 0 times 11197 (4.98%) aligned exactly 1 time 2507 (1.12%) aligned >1 times 95.17% overall alignment rate Time searching: 00:01:41 Overall time: 00:01:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 36398 / 2059107 = 0.0177 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:02: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:02: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:12: 1000000 INFO @ Wed, 22 Apr 2020 08:14:20: 2000000 INFO @ Wed, 22 Apr 2020 08:14:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:33: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:33: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:37: 4000000 INFO @ Wed, 22 Apr 2020 08:14:37: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:14:37: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:14:37: #1 total tags in treatment: 2031570 INFO @ Wed, 22 Apr 2020 08:14:37: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:14:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:14:37: #1 tags after filtering in treatment: 1923711 INFO @ Wed, 22 Apr 2020 08:14:37: #1 Redundant rate of treatment: 0.05 INFO @ Wed, 22 Apr 2020 08:14:37: #1 finished! INFO @ Wed, 22 Apr 2020 08:14:37: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:14:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:14:38: #2 number of paired peaks: 6 WARNING @ Wed, 22 Apr 2020 08:14:38: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:14:38: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 08:14:41: 1000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:14:48: 2000000 INFO @ Wed, 22 Apr 2020 08:14:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:15:01: 4000000 INFO @ Wed, 22 Apr 2020 08:15:01: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:15:01: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:15:01: #1 total tags in treatment: 2031570 INFO @ Wed, 22 Apr 2020 08:15:01: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:15:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:15:01: #1 tags after filtering in treatment: 1923711 INFO @ Wed, 22 Apr 2020 08:15:01: #1 Redundant rate of treatment: 0.05 INFO @ Wed, 22 Apr 2020 08:15:01: #1 finished! INFO @ Wed, 22 Apr 2020 08:15:01: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:15:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:15:02: #2 number of paired peaks: 6 WARNING @ Wed, 22 Apr 2020 08:15:02: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:15:02: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 08:15:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:15:03: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:15:03: #1 read treatment tags... cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:15:10: 1000000 INFO @ Wed, 22 Apr 2020 08:15:16: 2000000 INFO @ Wed, 22 Apr 2020 08:15:23: 3000000 INFO @ Wed, 22 Apr 2020 08:15:30: 4000000 INFO @ Wed, 22 Apr 2020 08:15:30: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:15:30: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:15:30: #1 total tags in treatment: 2031570 INFO @ Wed, 22 Apr 2020 08:15:30: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:15:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:15:30: #1 tags after filtering in treatment: 1923711 INFO @ Wed, 22 Apr 2020 08:15:30: #1 Redundant rate of treatment: 0.05 INFO @ Wed, 22 Apr 2020 08:15:30: #1 finished! INFO @ Wed, 22 Apr 2020 08:15:30: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:15:31: #2 number of paired peaks: 6 WARNING @ Wed, 22 Apr 2020 08:15:31: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:15:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874485/SRX5874485.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。