Job ID = 5790960 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 2,839,159 reads read : 5,678,318 reads written : 5,678,318 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:03 2839159 reads; of these: 2839159 (100.00%) were paired; of these: 92809 (3.27%) aligned concordantly 0 times 2460672 (86.67%) aligned concordantly exactly 1 time 285678 (10.06%) aligned concordantly >1 times ---- 92809 pairs aligned concordantly 0 times; of these: 2863 (3.08%) aligned discordantly 1 time ---- 89946 pairs aligned 0 times concordantly or discordantly; of these: 179892 mates make up the pairs; of these: 155494 (86.44%) aligned 0 times 19454 (10.81%) aligned exactly 1 time 4944 (2.75%) aligned >1 times 97.26% overall alignment rate Time searching: 00:02:03 Overall time: 00:02:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 24872 / 2746790 = 0.0091 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:13:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:13:46: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:13:46: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:13:52: 1000000 INFO @ Wed, 22 Apr 2020 08:13:59: 2000000 INFO @ Wed, 22 Apr 2020 08:14:06: 3000000 INFO @ Wed, 22 Apr 2020 08:14:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:16: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:16: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:19: 5000000 INFO @ Wed, 22 Apr 2020 08:14:22: 1000000 INFO @ Wed, 22 Apr 2020 08:14:22: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:14:22: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:14:22: #1 total tags in treatment: 2721486 INFO @ Wed, 22 Apr 2020 08:14:22: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:14:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:14:22: #1 tags after filtering in treatment: 2509955 INFO @ Wed, 22 Apr 2020 08:14:22: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:14:22: #1 finished! INFO @ Wed, 22 Apr 2020 08:14:22: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:14:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:14:22: #2 number of paired peaks: 31 WARNING @ Wed, 22 Apr 2020 08:14:22: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:14:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:14:28: 2000000 INFO @ Wed, 22 Apr 2020 08:14:33: 3000000 INFO @ Wed, 22 Apr 2020 08:14:39: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:45: 5000000 INFO @ Wed, 22 Apr 2020 08:14:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:46: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:46: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:48: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:14:48: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:14:48: #1 total tags in treatment: 2721486 INFO @ Wed, 22 Apr 2020 08:14:48: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:14:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:14:48: #1 tags after filtering in treatment: 2509955 INFO @ Wed, 22 Apr 2020 08:14:48: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:14:48: #1 finished! INFO @ Wed, 22 Apr 2020 08:14:48: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:14:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:14:48: #2 number of paired peaks: 31 WARNING @ Wed, 22 Apr 2020 08:14:48: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:14:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 08:14:52: 1000000 INFO @ Wed, 22 Apr 2020 08:14:58: 2000000 INFO @ Wed, 22 Apr 2020 08:15:03: 3000000 INFO @ Wed, 22 Apr 2020 08:15:09: 4000000 INFO @ Wed, 22 Apr 2020 08:15:15: 5000000 INFO @ Wed, 22 Apr 2020 08:15:17: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:15:17: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:15:17: #1 total tags in treatment: 2721486 INFO @ Wed, 22 Apr 2020 08:15:17: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:15:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:15:17: #1 tags after filtering in treatment: 2509955 INFO @ Wed, 22 Apr 2020 08:15:17: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:15:17: #1 finished! INFO @ Wed, 22 Apr 2020 08:15:17: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:15:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:15:17: #2 number of paired peaks: 31 WARNING @ Wed, 22 Apr 2020 08:15:17: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:15:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874473/SRX5874473.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。