Job ID = 5790949 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 2,624,668 reads read : 5,249,336 reads written : 5,249,336 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:03 2624668 reads; of these: 2624668 (100.00%) were paired; of these: 171151 (6.52%) aligned concordantly 0 times 2185858 (83.28%) aligned concordantly exactly 1 time 267659 (10.20%) aligned concordantly >1 times ---- 171151 pairs aligned concordantly 0 times; of these: 17716 (10.35%) aligned discordantly 1 time ---- 153435 pairs aligned 0 times concordantly or discordantly; of these: 306870 mates make up the pairs; of these: 278213 (90.66%) aligned 0 times 19838 (6.46%) aligned exactly 1 time 8819 (2.87%) aligned >1 times 94.70% overall alignment rate Time searching: 00:02:03 Overall time: 00:02:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 45596 / 2451978 = 0.0186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:13:32: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:13:32: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:13:37: 1000000 INFO @ Wed, 22 Apr 2020 08:13:42: 2000000 INFO @ Wed, 22 Apr 2020 08:13:47: 3000000 INFO @ Wed, 22 Apr 2020 08:13:52: 4000000 INFO @ Wed, 22 Apr 2020 08:13:57: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:13:57: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:13:57: #1 total tags in treatment: 2407953 INFO @ Wed, 22 Apr 2020 08:13:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:13:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:13:57: #1 tags after filtering in treatment: 2225268 INFO @ Wed, 22 Apr 2020 08:13:57: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:13:57: #1 finished! INFO @ Wed, 22 Apr 2020 08:13:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:13:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:13:57: #2 number of paired peaks: 34 WARNING @ Wed, 22 Apr 2020 08:13:57: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:13:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:02: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:02: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:07: 1000000 INFO @ Wed, 22 Apr 2020 08:14:12: 2000000 INFO @ Wed, 22 Apr 2020 08:14:17: 3000000 INFO @ Wed, 22 Apr 2020 08:14:22: 4000000 INFO @ Wed, 22 Apr 2020 08:14:26: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:14:26: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:14:26: #1 total tags in treatment: 2407953 INFO @ Wed, 22 Apr 2020 08:14:26: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:14:27: #1 tags after filtering in treatment: 2225268 INFO @ Wed, 22 Apr 2020 08:14:27: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:14:27: #1 finished! INFO @ Wed, 22 Apr 2020 08:14:27: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:14:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:14:27: #2 number of paired peaks: 34 WARNING @ Wed, 22 Apr 2020 08:14:27: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:14:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:14:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:14:32: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:14:32: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:14:37: 1000000 INFO @ Wed, 22 Apr 2020 08:14:42: 2000000 INFO @ Wed, 22 Apr 2020 08:14:47: 3000000 INFO @ Wed, 22 Apr 2020 08:14:52: 4000000 INFO @ Wed, 22 Apr 2020 08:14:57: #1 tag size is determined as 74 bps INFO @ Wed, 22 Apr 2020 08:14:57: #1 tag size = 74 INFO @ Wed, 22 Apr 2020 08:14:57: #1 total tags in treatment: 2407953 INFO @ Wed, 22 Apr 2020 08:14:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:14:57: #1 tags after filtering in treatment: 2225268 INFO @ Wed, 22 Apr 2020 08:14:57: #1 Redundant rate of treatment: 0.08 INFO @ Wed, 22 Apr 2020 08:14:57: #1 finished! INFO @ Wed, 22 Apr 2020 08:14:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:14:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:14:57: #2 number of paired peaks: 34 WARNING @ Wed, 22 Apr 2020 08:14:57: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:14:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5874465/SRX5874465.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。