Job ID = 7098622 SRX = SRX5854417 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7660179 spots for SRR9079159/SRR9079159.sra Written 7660179 spots for SRR9079159/SRR9079159.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:07 7660179 reads; of these: 7660179 (100.00%) were unpaired; of these: 848269 (11.07%) aligned 0 times 5558473 (72.56%) aligned exactly 1 time 1253437 (16.36%) aligned >1 times 88.93% overall alignment rate Time searching: 00:01:07 Overall time: 00:01:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1679766 / 6811910 = 0.2466 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:01:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:01:15: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:01:15: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:01:21: 1000000 INFO @ Wed, 22 Jul 2020 12:01:27: 2000000 INFO @ Wed, 22 Jul 2020 12:01:33: 3000000 INFO @ Wed, 22 Jul 2020 12:01:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:01:44: 5000000 INFO @ Wed, 22 Jul 2020 12:01:44: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:01:44: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:01:44: #1 total tags in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:01:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:01:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:01:44: #1 tags after filtering in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:01:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:01:44: #1 finished! INFO @ Wed, 22 Jul 2020 12:01:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:01:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:01:45: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:01:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:01:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:01:45: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:01:45: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:01:51: 1000000 INFO @ Wed, 22 Jul 2020 12:01:58: 2000000 INFO @ Wed, 22 Jul 2020 12:02:05: 3000000 INFO @ Wed, 22 Jul 2020 12:02:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:02:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:02:15: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:02:15: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:02:18: 5000000 INFO @ Wed, 22 Jul 2020 12:02:19: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:02:19: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:02:19: #1 total tags in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:02:19: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:02:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:02:19: #1 tags after filtering in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:02:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:02:19: #1 finished! INFO @ Wed, 22 Jul 2020 12:02:19: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:02:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:02:19: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:02:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:02:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:02:22: 1000000 INFO @ Wed, 22 Jul 2020 12:02:29: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:02:35: 3000000 INFO @ Wed, 22 Jul 2020 12:02:42: 4000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:02:48: 5000000 INFO @ Wed, 22 Jul 2020 12:02:49: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:02:49: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:02:49: #1 total tags in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:02:49: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:02:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:02:49: #1 tags after filtering in treatment: 5132144 INFO @ Wed, 22 Jul 2020 12:02:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:02:49: #1 finished! INFO @ Wed, 22 Jul 2020 12:02:49: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:02:49: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:02:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:02:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854417/SRX5854417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling