Job ID = 7098484 SRX = SRX5854414 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8443764 spots for SRR9079156/SRR9079156.sra Written 8443764 spots for SRR9079156/SRR9079156.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:02 8443764 reads; of these: 8443764 (100.00%) were unpaired; of these: 287037 (3.40%) aligned 0 times 7220426 (85.51%) aligned exactly 1 time 936301 (11.09%) aligned >1 times 96.60% overall alignment rate Time searching: 00:01:02 Overall time: 00:01:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1882066 / 8156727 = 0.2307 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:59:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:59:42: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:59:42: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:59:47: 1000000 INFO @ Wed, 22 Jul 2020 11:59:53: 2000000 INFO @ Wed, 22 Jul 2020 11:59:59: 3000000 INFO @ Wed, 22 Jul 2020 12:00:04: 4000000 INFO @ Wed, 22 Jul 2020 12:00:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:00:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:00:12: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:00:12: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:00:16: 6000000 INFO @ Wed, 22 Jul 2020 12:00:18: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:00:18: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:00:18: #1 total tags in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:00:18: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:00:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:00:18: #1 tags after filtering in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:00:18: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:00:18: #1 finished! INFO @ Wed, 22 Jul 2020 12:00:18: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:00:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:00:18: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:00:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:00:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:00:19: 1000000 INFO @ Wed, 22 Jul 2020 12:00:26: 2000000 INFO @ Wed, 22 Jul 2020 12:00:33: 3000000 INFO @ Wed, 22 Jul 2020 12:00:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:00:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:00:42: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:00:42: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:00:47: 5000000 INFO @ Wed, 22 Jul 2020 12:00:49: 1000000 INFO @ Wed, 22 Jul 2020 12:00:55: 6000000 INFO @ Wed, 22 Jul 2020 12:00:56: 2000000 INFO @ Wed, 22 Jul 2020 12:00:57: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:00:57: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:00:57: #1 total tags in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:00:57: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:00:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:00:57: #1 tags after filtering in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:00:57: #1 finished! INFO @ Wed, 22 Jul 2020 12:00:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:00:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:00:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:00:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:01:03: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:01:09: 4000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:01:14: 5000000 INFO @ Wed, 22 Jul 2020 12:01:20: 6000000 INFO @ Wed, 22 Jul 2020 12:01:21: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:01:21: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:01:21: #1 total tags in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:01:21: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:01:21: #1 tags after filtering in treatment: 6274661 INFO @ Wed, 22 Jul 2020 12:01:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:01:21: #1 finished! INFO @ Wed, 22 Jul 2020 12:01:21: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:01:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:01:22: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:01:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:01:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5854414/SRX5854414.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling