Job ID = 2641228 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 6,609,648 reads read : 13,219,296 reads written : 13,219,296 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:36 6609648 reads; of these: 6609648 (100.00%) were paired; of these: 6369275 (96.36%) aligned concordantly 0 times 210843 (3.19%) aligned concordantly exactly 1 time 29530 (0.45%) aligned concordantly >1 times ---- 6369275 pairs aligned concordantly 0 times; of these: 291736 (4.58%) aligned discordantly 1 time ---- 6077539 pairs aligned 0 times concordantly or discordantly; of these: 12155078 mates make up the pairs; of these: 10584090 (87.08%) aligned 0 times 1394451 (11.47%) aligned exactly 1 time 176537 (1.45%) aligned >1 times 19.93% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 321958 / 528774 = 0.6089 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 22:06:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:06:24: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:06:24: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:06:30: 1000000 INFO @ Sat, 24 Aug 2019 22:06:37: #1 tag size is determined as 41 bps INFO @ Sat, 24 Aug 2019 22:06:37: #1 tag size = 41 INFO @ Sat, 24 Aug 2019 22:06:37: #1 total tags in treatment: 151358 INFO @ Sat, 24 Aug 2019 22:06:37: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:06:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:06:37: #1 tags after filtering in treatment: 139623 INFO @ Sat, 24 Aug 2019 22:06:37: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 24 Aug 2019 22:06:37: #1 finished! INFO @ Sat, 24 Aug 2019 22:06:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:06:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:06:37: #2 number of paired peaks: 99 WARNING @ Sat, 24 Aug 2019 22:06:37: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:06:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 22:06:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:06:54: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:06:54: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:07:00: 1000000 INFO @ Sat, 24 Aug 2019 22:07:07: #1 tag size is determined as 41 bps INFO @ Sat, 24 Aug 2019 22:07:07: #1 tag size = 41 INFO @ Sat, 24 Aug 2019 22:07:07: #1 total tags in treatment: 151358 INFO @ Sat, 24 Aug 2019 22:07:07: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:07:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:07:07: #1 tags after filtering in treatment: 139623 INFO @ Sat, 24 Aug 2019 22:07:07: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 24 Aug 2019 22:07:07: #1 finished! INFO @ Sat, 24 Aug 2019 22:07:07: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:07:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:07:07: #2 number of paired peaks: 99 WARNING @ Sat, 24 Aug 2019 22:07:07: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:07:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Sat, 24 Aug 2019 22:07:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 22:07:24: #1 read tag files... INFO @ Sat, 24 Aug 2019 22:07:24: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 22:07:30: 1000000 INFO @ Sat, 24 Aug 2019 22:07:37: #1 tag size is determined as 41 bps INFO @ Sat, 24 Aug 2019 22:07:37: #1 tag size = 41 INFO @ Sat, 24 Aug 2019 22:07:37: #1 total tags in treatment: 151358 INFO @ Sat, 24 Aug 2019 22:07:37: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 22:07:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 22:07:37: #1 tags after filtering in treatment: 139623 INFO @ Sat, 24 Aug 2019 22:07:37: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 24 Aug 2019 22:07:37: #1 finished! INFO @ Sat, 24 Aug 2019 22:07:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 22:07:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 22:07:37: #2 number of paired peaks: 99 WARNING @ Sat, 24 Aug 2019 22:07:37: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 22:07:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5820062/SRX5820062.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。