Job ID = 12531717 SRX = SRX5811098 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24809065 spots for SRR9033914/SRR9033914.sra Written 24809065 spots for SRR9033914/SRR9033914.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:20 24809065 reads; of these: 24809065 (100.00%) were paired; of these: 13921296 (56.11%) aligned concordantly 0 times 8780553 (35.39%) aligned concordantly exactly 1 time 2107216 (8.49%) aligned concordantly >1 times ---- 13921296 pairs aligned concordantly 0 times; of these: 819541 (5.89%) aligned discordantly 1 time ---- 13101755 pairs aligned 0 times concordantly or discordantly; of these: 26203510 mates make up the pairs; of these: 25232766 (96.30%) aligned 0 times 267820 (1.02%) aligned exactly 1 time 702924 (2.68%) aligned >1 times 49.15% overall alignment rate Time searching: 00:16:20 Overall time: 00:16:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2693816 / 11669716 = 0.2308 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:44:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:44:47: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:44:47: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:44:55: 1000000 INFO @ Sat, 17 Apr 2021 09:45:03: 2000000 INFO @ Sat, 17 Apr 2021 09:45:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:45:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:45:17: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:45:17: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:45:19: 4000000 INFO @ Sat, 17 Apr 2021 09:45:27: 1000000 INFO @ Sat, 17 Apr 2021 09:45:28: 5000000 INFO @ Sat, 17 Apr 2021 09:45:37: 2000000 INFO @ Sat, 17 Apr 2021 09:45:38: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:45:47: 3000000 INFO @ Sat, 17 Apr 2021 09:45:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:45:47: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:45:47: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:45:47: 7000000 INFO @ Sat, 17 Apr 2021 09:45:57: 4000000 INFO @ Sat, 17 Apr 2021 09:45:57: 8000000 INFO @ Sat, 17 Apr 2021 09:45:57: 1000000 INFO @ Sat, 17 Apr 2021 09:46:07: 9000000 INFO @ Sat, 17 Apr 2021 09:46:07: 5000000 INFO @ Sat, 17 Apr 2021 09:46:08: 2000000 INFO @ Sat, 17 Apr 2021 09:46:17: 10000000 INFO @ Sat, 17 Apr 2021 09:46:18: 6000000 INFO @ Sat, 17 Apr 2021 09:46:18: 3000000 INFO @ Sat, 17 Apr 2021 09:46:27: 11000000 INFO @ Sat, 17 Apr 2021 09:46:28: 7000000 INFO @ Sat, 17 Apr 2021 09:46:29: 4000000 INFO @ Sat, 17 Apr 2021 09:46:37: 12000000 INFO @ Sat, 17 Apr 2021 09:46:39: 8000000 INFO @ Sat, 17 Apr 2021 09:46:39: 5000000 INFO @ Sat, 17 Apr 2021 09:46:47: 13000000 INFO @ Sat, 17 Apr 2021 09:46:49: 9000000 INFO @ Sat, 17 Apr 2021 09:46:49: 6000000 INFO @ Sat, 17 Apr 2021 09:46:56: 14000000 INFO @ Sat, 17 Apr 2021 09:46:59: 10000000 INFO @ Sat, 17 Apr 2021 09:47:00: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:47:06: 15000000 INFO @ Sat, 17 Apr 2021 09:47:10: 11000000 INFO @ Sat, 17 Apr 2021 09:47:10: 8000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:47:16: 16000000 INFO @ Sat, 17 Apr 2021 09:47:20: 12000000 INFO @ Sat, 17 Apr 2021 09:47:21: 9000000 INFO @ Sat, 17 Apr 2021 09:47:26: 17000000 INFO @ Sat, 17 Apr 2021 09:47:30: 13000000 INFO @ Sat, 17 Apr 2021 09:47:31: 10000000 INFO @ Sat, 17 Apr 2021 09:47:35: 18000000 INFO @ Sat, 17 Apr 2021 09:47:41: 14000000 INFO @ Sat, 17 Apr 2021 09:47:42: 11000000 INFO @ Sat, 17 Apr 2021 09:47:45: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:47:45: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:47:45: #1 total tags in treatment: 8324698 INFO @ Sat, 17 Apr 2021 09:47:45: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:47:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:47:45: #1 tags after filtering in treatment: 4933681 INFO @ Sat, 17 Apr 2021 09:47:45: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 17 Apr 2021 09:47:45: #1 finished! INFO @ Sat, 17 Apr 2021 09:47:45: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:47:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:47:46: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:47:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:47:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:47:51: 15000000 INFO @ Sat, 17 Apr 2021 09:47:52: 12000000 INFO @ Sat, 17 Apr 2021 09:48:01: 16000000 INFO @ Sat, 17 Apr 2021 09:48:02: 13000000 INFO @ Sat, 17 Apr 2021 09:48:11: 17000000 INFO @ Sat, 17 Apr 2021 09:48:12: 14000000 INFO @ Sat, 17 Apr 2021 09:48:21: 18000000 INFO @ Sat, 17 Apr 2021 09:48:22: 15000000 INFO @ Sat, 17 Apr 2021 09:48:30: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:48:30: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:48:30: #1 total tags in treatment: 8324698 INFO @ Sat, 17 Apr 2021 09:48:30: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:48:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:48:31: #1 tags after filtering in treatment: 4933681 INFO @ Sat, 17 Apr 2021 09:48:31: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 17 Apr 2021 09:48:31: #1 finished! INFO @ Sat, 17 Apr 2021 09:48:31: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:48:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:48:31: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:48:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:48:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:48:32: 16000000 INFO @ Sat, 17 Apr 2021 09:48:41: 17000000 INFO @ Sat, 17 Apr 2021 09:48:51: 18000000 INFO @ Sat, 17 Apr 2021 09:48:59: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:48:59: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:48:59: #1 total tags in treatment: 8324698 INFO @ Sat, 17 Apr 2021 09:48:59: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:48:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:48:59: #1 tags after filtering in treatment: 4933681 INFO @ Sat, 17 Apr 2021 09:48:59: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 17 Apr 2021 09:48:59: #1 finished! INFO @ Sat, 17 Apr 2021 09:48:59: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:48:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:49:00: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:49:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:49:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811098/SRX5811098.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling