Job ID = 12531716 SRX = SRX5811097 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22315966 spots for SRR9033913/SRR9033913.sra Written 22315966 spots for SRR9033913/SRR9033913.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:12:25 22315966 reads; of these: 22315966 (100.00%) were paired; of these: 12354162 (55.36%) aligned concordantly 0 times 8184606 (36.68%) aligned concordantly exactly 1 time 1777198 (7.96%) aligned concordantly >1 times ---- 12354162 pairs aligned concordantly 0 times; of these: 907730 (7.35%) aligned discordantly 1 time ---- 11446432 pairs aligned 0 times concordantly or discordantly; of these: 22892864 mates make up the pairs; of these: 21994503 (96.08%) aligned 0 times 260607 (1.14%) aligned exactly 1 time 637754 (2.79%) aligned >1 times 50.72% overall alignment rate Time searching: 00:12:26 Overall time: 00:12:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2353201 / 10835223 = 0.2172 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:39:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:39:10: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:39:10: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:39:16: 1000000 INFO @ Sat, 17 Apr 2021 09:39:22: 2000000 INFO @ Sat, 17 Apr 2021 09:39:28: 3000000 INFO @ Sat, 17 Apr 2021 09:39:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:39:40: 5000000 INFO @ Sat, 17 Apr 2021 09:39:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:39:40: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:39:40: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:39:47: 6000000 INFO @ Sat, 17 Apr 2021 09:39:49: 1000000 INFO @ Sat, 17 Apr 2021 09:39:54: 7000000 INFO @ Sat, 17 Apr 2021 09:39:57: 2000000 INFO @ Sat, 17 Apr 2021 09:40:01: 8000000 INFO @ Sat, 17 Apr 2021 09:40:06: 3000000 BedGraph に変換中... INFO @ Sat, 17 Apr 2021 09:40:09: 9000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:40:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:40:10: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:40:10: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:40:14: 4000000 INFO @ Sat, 17 Apr 2021 09:40:16: 10000000 INFO @ Sat, 17 Apr 2021 09:40:19: 1000000 INFO @ Sat, 17 Apr 2021 09:40:23: 5000000 INFO @ Sat, 17 Apr 2021 09:40:23: 11000000 INFO @ Sat, 17 Apr 2021 09:40:27: 2000000 INFO @ Sat, 17 Apr 2021 09:40:30: 12000000 INFO @ Sat, 17 Apr 2021 09:40:31: 6000000 INFO @ Sat, 17 Apr 2021 09:40:36: 3000000 INFO @ Sat, 17 Apr 2021 09:40:37: 13000000 INFO @ Sat, 17 Apr 2021 09:40:40: 7000000 INFO @ Sat, 17 Apr 2021 09:40:44: 4000000 INFO @ Sat, 17 Apr 2021 09:40:45: 14000000 INFO @ Sat, 17 Apr 2021 09:40:49: 8000000 INFO @ Sat, 17 Apr 2021 09:40:52: 15000000 INFO @ Sat, 17 Apr 2021 09:40:53: 5000000 INFO @ Sat, 17 Apr 2021 09:40:57: 9000000 INFO @ Sat, 17 Apr 2021 09:40:59: 16000000 INFO @ Sat, 17 Apr 2021 09:41:01: 6000000 INFO @ Sat, 17 Apr 2021 09:41:06: 10000000 INFO @ Sat, 17 Apr 2021 09:41:06: 17000000 INFO @ Sat, 17 Apr 2021 09:41:10: 7000000 INFO @ Sat, 17 Apr 2021 09:41:13: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:41:13: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:41:13: #1 total tags in treatment: 7744514 INFO @ Sat, 17 Apr 2021 09:41:13: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:41:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:41:13: #1 tags after filtering in treatment: 4717002 INFO @ Sat, 17 Apr 2021 09:41:13: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 17 Apr 2021 09:41:13: #1 finished! INFO @ Sat, 17 Apr 2021 09:41:13: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:41:13: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:41:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:41:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:41:14: 11000000 INFO @ Sat, 17 Apr 2021 09:41:18: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:41:22: 12000000 INFO @ Sat, 17 Apr 2021 09:41:26: 9000000 INFO @ Sat, 17 Apr 2021 09:41:31: 13000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:41:35: 10000000 INFO @ Sat, 17 Apr 2021 09:41:39: 14000000 INFO @ Sat, 17 Apr 2021 09:41:43: 11000000 INFO @ Sat, 17 Apr 2021 09:41:47: 15000000 INFO @ Sat, 17 Apr 2021 09:41:51: 12000000 INFO @ Sat, 17 Apr 2021 09:41:56: 16000000 INFO @ Sat, 17 Apr 2021 09:41:59: 13000000 INFO @ Sat, 17 Apr 2021 09:42:04: 17000000 INFO @ Sat, 17 Apr 2021 09:42:08: 14000000 INFO @ Sat, 17 Apr 2021 09:42:11: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:42:11: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:42:11: #1 total tags in treatment: 7744514 INFO @ Sat, 17 Apr 2021 09:42:11: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:42:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:42:12: #1 tags after filtering in treatment: 4717002 INFO @ Sat, 17 Apr 2021 09:42:12: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 17 Apr 2021 09:42:12: #1 finished! INFO @ Sat, 17 Apr 2021 09:42:12: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:42:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:42:12: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:42:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:42:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:42:16: 15000000 INFO @ Sat, 17 Apr 2021 09:42:24: 16000000 INFO @ Sat, 17 Apr 2021 09:42:32: 17000000 INFO @ Sat, 17 Apr 2021 09:42:39: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:42:39: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:42:39: #1 total tags in treatment: 7744514 INFO @ Sat, 17 Apr 2021 09:42:39: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:42:39: #1 tags after filtering in treatment: 4717002 INFO @ Sat, 17 Apr 2021 09:42:39: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 17 Apr 2021 09:42:39: #1 finished! INFO @ Sat, 17 Apr 2021 09:42:39: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:42:39: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:42:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:42:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811097/SRX5811097.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling