Job ID = 12531715 SRX = SRX5811096 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30294934 spots for SRR9033912/SRR9033912.sra Written 30294934 spots for SRR9033912/SRR9033912.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:30 30294934 reads; of these: 30294934 (100.00%) were paired; of these: 16589306 (54.76%) aligned concordantly 0 times 11078584 (36.57%) aligned concordantly exactly 1 time 2627044 (8.67%) aligned concordantly >1 times ---- 16589306 pairs aligned concordantly 0 times; of these: 1248982 (7.53%) aligned discordantly 1 time ---- 15340324 pairs aligned 0 times concordantly or discordantly; of these: 30680648 mates make up the pairs; of these: 29336315 (95.62%) aligned 0 times 357133 (1.16%) aligned exactly 1 time 987200 (3.22%) aligned >1 times 51.58% overall alignment rate Time searching: 00:23:30 Overall time: 00:23:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3510474 / 14911525 = 0.2354 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:55:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:55:00: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:55:00: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:55:06: 1000000 INFO @ Sat, 17 Apr 2021 09:55:12: 2000000 INFO @ Sat, 17 Apr 2021 09:55:19: 3000000 INFO @ Sat, 17 Apr 2021 09:55:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:55:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:55:29: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:55:29: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:55:31: 5000000 INFO @ Sat, 17 Apr 2021 09:55:36: 1000000 INFO @ Sat, 17 Apr 2021 09:55:38: 6000000 INFO @ Sat, 17 Apr 2021 09:55:43: 2000000 INFO @ Sat, 17 Apr 2021 09:55:45: 7000000 INFO @ Sat, 17 Apr 2021 09:55:50: 3000000 INFO @ Sat, 17 Apr 2021 09:55:51: 8000000 INFO @ Sat, 17 Apr 2021 09:55:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:55:58: 9000000 INFO @ Sat, 17 Apr 2021 09:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:56:00: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:56:00: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:56:03: 5000000 INFO @ Sat, 17 Apr 2021 09:56:05: 10000000 INFO @ Sat, 17 Apr 2021 09:56:06: 1000000 INFO @ Sat, 17 Apr 2021 09:56:09: 6000000 INFO @ Sat, 17 Apr 2021 09:56:12: 11000000 INFO @ Sat, 17 Apr 2021 09:56:13: 2000000 INFO @ Sat, 17 Apr 2021 09:56:16: 7000000 INFO @ Sat, 17 Apr 2021 09:56:19: 12000000 INFO @ Sat, 17 Apr 2021 09:56:21: 3000000 INFO @ Sat, 17 Apr 2021 09:56:23: 8000000 INFO @ Sat, 17 Apr 2021 09:56:26: 13000000 INFO @ Sat, 17 Apr 2021 09:56:28: 4000000 INFO @ Sat, 17 Apr 2021 09:56:29: 9000000 INFO @ Sat, 17 Apr 2021 09:56:33: 14000000 INFO @ Sat, 17 Apr 2021 09:56:35: 5000000 INFO @ Sat, 17 Apr 2021 09:56:36: 10000000 INFO @ Sat, 17 Apr 2021 09:56:40: 15000000 INFO @ Sat, 17 Apr 2021 09:56:41: 6000000 INFO @ Sat, 17 Apr 2021 09:56:43: 11000000 INFO @ Sat, 17 Apr 2021 09:56:47: 16000000 INFO @ Sat, 17 Apr 2021 09:56:48: 7000000 INFO @ Sat, 17 Apr 2021 09:56:50: 12000000 INFO @ Sat, 17 Apr 2021 09:56:54: 17000000 INFO @ Sat, 17 Apr 2021 09:56:55: 8000000 INFO @ Sat, 17 Apr 2021 09:56:56: 13000000 INFO @ Sat, 17 Apr 2021 09:57:01: 18000000 INFO @ Sat, 17 Apr 2021 09:57:02: 9000000 INFO @ Sat, 17 Apr 2021 09:57:03: 14000000 INFO @ Sat, 17 Apr 2021 09:57:08: 19000000 INFO @ Sat, 17 Apr 2021 09:57:09: 10000000 INFO @ Sat, 17 Apr 2021 09:57:10: 15000000 INFO @ Sat, 17 Apr 2021 09:57:15: 20000000 INFO @ Sat, 17 Apr 2021 09:57:16: 16000000 INFO @ Sat, 17 Apr 2021 09:57:16: 11000000 INFO @ Sat, 17 Apr 2021 09:57:22: 21000000 INFO @ Sat, 17 Apr 2021 09:57:23: 17000000 INFO @ Sat, 17 Apr 2021 09:57:23: 12000000 INFO @ Sat, 17 Apr 2021 09:57:29: 22000000 INFO @ Sat, 17 Apr 2021 09:57:30: 18000000 INFO @ Sat, 17 Apr 2021 09:57:30: 13000000 INFO @ Sat, 17 Apr 2021 09:57:36: 23000000 INFO @ Sat, 17 Apr 2021 09:57:36: 19000000 INFO @ Sat, 17 Apr 2021 09:57:37: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:57:42: 24000000 INFO @ Sat, 17 Apr 2021 09:57:43: 20000000 INFO @ Sat, 17 Apr 2021 09:57:44: 15000000 INFO @ Sat, 17 Apr 2021 09:57:44: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:57:44: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:57:44: #1 total tags in treatment: 10393999 INFO @ Sat, 17 Apr 2021 09:57:44: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:57:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:57:44: #1 tags after filtering in treatment: 5732993 INFO @ Sat, 17 Apr 2021 09:57:44: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 17 Apr 2021 09:57:44: #1 finished! INFO @ Sat, 17 Apr 2021 09:57:44: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:57:45: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:57:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:57:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:57:49: 21000000 INFO @ Sat, 17 Apr 2021 09:57:50: 16000000 INFO @ Sat, 17 Apr 2021 09:57:56: 22000000 INFO @ Sat, 17 Apr 2021 09:57:57: 17000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:58:02: 23000000 INFO @ Sat, 17 Apr 2021 09:58:03: 18000000 INFO @ Sat, 17 Apr 2021 09:58:09: 24000000 INFO @ Sat, 17 Apr 2021 09:58:10: 19000000 INFO @ Sat, 17 Apr 2021 09:58:11: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:58:11: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:58:11: #1 total tags in treatment: 10393999 INFO @ Sat, 17 Apr 2021 09:58:11: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:58:11: #1 tags after filtering in treatment: 5732993 INFO @ Sat, 17 Apr 2021 09:58:11: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 17 Apr 2021 09:58:11: #1 finished! INFO @ Sat, 17 Apr 2021 09:58:11: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:58:11: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:58:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:58:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:58:17: 20000000 INFO @ Sat, 17 Apr 2021 09:58:23: 21000000 INFO @ Sat, 17 Apr 2021 09:58:29: 22000000 INFO @ Sat, 17 Apr 2021 09:58:35: 23000000 INFO @ Sat, 17 Apr 2021 09:58:42: 24000000 INFO @ Sat, 17 Apr 2021 09:58:43: #1 tag size is determined as 100 bps INFO @ Sat, 17 Apr 2021 09:58:43: #1 tag size = 100 INFO @ Sat, 17 Apr 2021 09:58:43: #1 total tags in treatment: 10393999 INFO @ Sat, 17 Apr 2021 09:58:43: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:58:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:58:44: #1 tags after filtering in treatment: 5732993 INFO @ Sat, 17 Apr 2021 09:58:44: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 17 Apr 2021 09:58:44: #1 finished! INFO @ Sat, 17 Apr 2021 09:58:44: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:58:44: #2 number of paired peaks: 0 WARNING @ Sat, 17 Apr 2021 09:58:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:58:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX5811096/SRX5811096.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling